Development of an enantioselective capillary electrophoretic method for the simultaneous determination of montelukast enantiomeric and diastereoisomeric forms and its main degradation product

2016 ◽  
Vol 37 (17-18) ◽  
pp. 2420-2428 ◽  
Author(s):  
Sabrina Flor ◽  
Martin Huala Juan ◽  
Valeria Tripodi ◽  
Silvia Lucangioli
Keyword(s):  
Simultaneous Determination ◽  
Degradation Product ◽  
Electrophoretic Method ◽  
Main Degradation Product ◽  
Capillary Electrophoretic

scholarly journals Development and Application of a Validated Stability-Indicating High-Performance Liquid Chromatographic Method Using Photodiode Array Detection for Simultaneous Determination of Granisetron, Methylparaben, Propylparaben, Sodium Benzoate, and Their Main Degradation Products in Oral Pharmaceutical Preparations

2011 ◽  
Vol 94 (5) ◽  
pp. 1447-1460 ◽  
Author(s):  
Ismail Hewala ◽  
Hamed El-Fatatry ◽  
Ehab Emam ◽  
Mokhtar Mabrouk
Keyword(s):  
Simultaneous Determination ◽  
Degradation Product ◽  
Sodium Benzoate ◽  
Degradation Products ◽  
Hydroxybenzoic Acid ◽  
Photodiode Array Detection ◽  
Main Degradation Product ◽  
Photodiode Array ◽  
Array Detection

Abstract A simple, rapid, and sensitive RP-HPLC method using photodiode array detection was developed and validated for the simultaneous determination of granisetron hydrochloride, 1-methyl-1H-indazole-3-carboxylic acid (the main degradation product of granisetron), sodium benzoate, methylparaben, propylparaben, and 4-hydroxybenzoic acid (the main degradation product of parabens) in granisetron oral drops and solutions. The separation of the compounds was achieved within 8 min on a SymmetryShield RP18 column (100 × 4.6 mm id, 3.5 μm particle size) using the mobile phase acetonitrile—0.05 M KH2PO4 buffered to pH 3 using H3PO4 (3+7, v/v). The photodiode array detector was used to test the purity of the peaks, and the chromatograms were extracted at 240 nm. The method was validated, and validation acceptance criteria were met in all cases. The robust method was successfully applied to the determination of granisetron and preservatives, as well as their degradation products in different batches of granisetron oral drops and solutions. The method proved to be sensitive for determination down to 0.04% (w/w) of granisetron degradation product relative to granisetron and 0.03% (w/w) 4-hydroxybenzoic acid relative to total parabens.

scholarly journals Synchronous fluorescence as a green and selective tool for simultaneous determination of bambuterol and its main degradation product, terbutaline

2018 ◽  
Vol 5 (10) ◽  
pp. 181359 ◽  
Author(s):  
Samah Abo El Abass ◽  
Heba Elmansi
Keyword(s):  
Degradation Product ◽  
Low Cost ◽  
Reference Method ◽  
Cost Effective ◽  
Zero Crossing ◽  
Cost Effective Method ◽  
Validation Parameters ◽  
Main Degradation Product ◽  
20 Nm

A green, sensitive and cost-effective method is introduced in this research for the determination of bambuterol and its main degradation product, terbutaline, simultaneously, relying on the synchronous spectrofluorimetric technique. First derivative synchronous spectrofluorimetric amplitude is measured at Δ λ = 20 nm, so bambuterol can be quantitated at 260 nm, and terbutaline can be measured at 290 nm, each at the zero crossing point of the other. The amplitude–concentration plots were linear over the concentration ranges of 0.2–6.0 µg ml −1 and 0.2–4.0 µg ml −1 for both bambuterol and terbutaline, respectively. Official guidelines were followed to calculate the validation parameters of the proposed method. The low values of limits of detection of 0.023, 0.056 µg ml −1 and limits of quantitation of 0.071, 0.169 µg ml −1 for bambuterol and terbutaline, respectively, point to the sensitivity of the method. Bambuterol is a prodrug for terbutaline, and the latter is considered its degradation product so the established method could be regarded as a stability-indicating one. Moreover, the proposed method was used for the analysis of bambuterol and terbutaline in their single ingredient preparations and the results revealed statistical agreement with the reference method. The suggested method, being a simple and low-cost procedure, is superior to the previously published methods which need more sophisticated techniques, longer analysis time and highly toxic solvents and reagents. It could be considered as an eco-friendly analytical procedure.

A sub-minute electrophoretic method for simultaneous determination of naphazoline and zinc

2016 ◽  
Vol 1472 ◽  
pp. 134-137 ◽  
Author(s):  
Michelle M.A.C. Ribeiro ◽  
Thiago C. Oliveira ◽  
Alex D. Batista ◽  
Rodrigo A.A. Muñoz ◽  
Eduardo M. Richter
Keyword(s):  
Simultaneous Determination ◽  
Electrophoretic Method
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