Determination of the binding constants of modafinil enantiomers with sulfated β-cyclodextrin chiral selector by capillary electrophoresis using three different linear plotting methods

2010 ◽  
Vol 31 (17) ◽  
pp. 2957-2963 ◽  
Author(s):  
Khaldun M. Al Azzam ◽  
Bahruddin Saad ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Capillary Electrophoresis ◽  
Binding Constants ◽  
Chiral Selector

scholarly journals Binding Constants Determination of Ofloxacin and Ornidazole Enantiomers with Sulfated β-Cyclodextrin as Single Ligand by Capillary Electrophoresis using Three Different Linear Plotting Methods

2021 ◽  
Vol 33 (7) ◽  
pp. 1663-1670
Author(s):  
Khaldun M. Al Azzam
Keyword(s):  
Capillary Electrophoresis ◽  
Background Electrolyte ◽  
Binding Constants ◽  
Chiral Selector ◽  
The Other ◽  
Binding Parameters ◽  
Guest Binding ◽  
Different Temperatures ◽  
Temperature Ranges

The aim of this research is to use capillary electrophoresis to establish/determine the binding constants for ofloxacin and ornidazole enantiomers with the negatively charged chiral selector sulfated-β-cyclodextrin (S-β-CD). Using electrophoretic mobility values of ofloxacin and ornidazole enantiomers at various concentrations of S-β-CD used in the context of background electrolyte (BGE), binding constants were calculated using three separate linearization plots, namely double-reciprocal, X-reciprocal and Y-reciprocal. The R-ofloxacin enantiomer-S-β-CD complex had the highest inclusion affinity of the ofloxacin and ornidazole enantiomers, which matched with previously reported estimation. Every enantiomer-S-β-CD complex’s binding constants, as well as thermodynamic binding parameters, were calculated at different temperatures. The host-guest binding constants using double reciprocal fit showed greater linearity (R2 > 0.99) at all temperature ranges measured (15-30 ºC) as compared to the other two fit approaches. The thermodynamic complexation parameters were found to be dependent on the temperature of the enantiomers, as seen by the linear van’t Hoff (15-30 ºC) plot.

Determination of enantiomeric excess for 2,3-dihydroxy-3-phenylpropionate compounds by capillary electrophoresis using hydroxypropyl-β-cyclodextrin as chiral selector

Chirality ◽  
2007 ◽  
Vol 19 (5) ◽  
pp. 380-385 ◽  
Author(s):  
Yan Zhao ◽  
Xing-Bin Yang ◽  
Qiao-Feng Wang ◽  
Peng-Juan Nan ◽  
Ying Jin ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Enantiomeric Excess ◽  
Chiral Selector

scholarly journals Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2879
Author(s):  
Pavel Řezanka ◽  
Denisa Macková ◽  
Radek Jurok ◽  
Michal Himl ◽  
Martin Kuchař
Keyword(s):  
Capillary Electrophoresis ◽  
Chiral Separation ◽  
Simplex Method ◽  
Background Electrolyte ◽  
Detection Limits ◽  
Chiral Selector ◽  
Designer Drug ◽  
Optimal Composition ◽  
Linear Calibration

Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug. The determination of mephedrone and its metabolites is important for understanding its possible use in medicine. In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed. Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates. Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L−1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved. For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L−1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel’s fitting test, the linear calibration ranges were determined.

Sign in / Sign up

Export Citation Format

Share Document