scholarly journals Template tailoring: Accurate determination of heterozygous alleles using peptide nucleic acid and dideoxyNTP

2010 ◽  
Vol 31 (8) ◽  
pp. 1322-1329
Author(s):  
Muhammad Akram Tariq ◽  
Nader Pourmand
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Accurate Determination

The use of spectroscopic techniques in the study of DNA stability

2000 ◽  
Author(s):  
John Jr SantaLucia
Keyword(s):  
Nucleic Acid ◽  
Diagnostic Tests ◽  
Accurate Determination ◽  
Spectroscopic Techniques ◽  
Dna And Rna ◽  
Repair Efficiency ◽  
Primary Literature ◽  
Folding Thermodynamics ◽  
Data Analysis Methods

Accurate determination of nucleic acid thermodynamics has become increasingly important in understanding biological function as well as applications in biotechnology and pharmaceuticals. Knowledge of the thermodynamics of DNA hybridization and secondary structure formation is necessary for understanding DNA replication fidelity (1), mismatch repair efficiency (2) and the mechanism of DNA triplet repeat diseases (3). In addition, RNA folding thermodynamics are an important aspect of understanding ribozyme catalysis, as well as understanding the regulation of protein expression, mRNA stability and the mechanism of protein synthesis by the ribosome (4). With the genome sequencing era upon us (5), it will increasingly become important to predict the folding and hybridization thermodynamics of DNA and RNA, so that accurate diagnostic tests for genetic and infectious diseases can be developed. Thus, there is a need to develop a database of accurate thermodynamic parameters for different nucleic acid folding motifs (4). This chapter describes practical aspects of the application of UV absorbance temperature profiles to determine the thermodynamics of nucleic acid structural transitions. Protocols and practical advice are presented for issues not normally addressed in the primary literature but that are crucial for the determination of reliable thermodynamics, such as sequence design, sample preparation, choice of buffer, protocols for determining strand concentrations and mixing strands, design of microvolume cuvettes and cell holder, instrumental requirements, data analysis methods, and sources of error. References to the primary literature and reviews are also provided where appropriate.

Electrochemical Determination of Hg2+Using Electrodes Modified with Peptide Nucleic Acid

2016 ◽  
Vol 163 (14) ◽  
pp. B667-B672 ◽  
Author(s):  
Agnieszka Bala ◽  
Krzysztof Zalewski ◽  
Łukasz Górski
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Electrochemical Determination

Preparation and determination of optical purity of γ-lysine modified peptide nucleic acid analogues

2012 ◽  
Vol 35 (3) ◽  
pp. 517-522 ◽  
Author(s):  
Hu Huang ◽  
Goon Ho Joe ◽  
Sung Rok Choi ◽  
Su Nam Kim ◽  
Yong Tae Kim ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Optical Purity ◽  
Nucleic Acid Analogues

scholarly journals Analysis of Inactivation of SARS-CoV-2 by Specimen Transport Media, Nucleic Acid Extraction Reagents, Detergents, and Fixatives

2020 ◽  
Vol 58 (11) ◽  
Author(s):  
Stephen R. Welch ◽  
Katherine A. Davies ◽  
Hubert Buczkowski ◽  
Nipunadi Hettiarachchi ◽  
Nicole Green ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Infectious Virus ◽  
Clinical Sample ◽  
Accurate Determination ◽  
Acid Extraction ◽  
The Novel ◽  
Nucleic Acid Extraction ◽  
Human Coronavirus ◽  
Critical Data

ABSTRACT The COVID-19 pandemic has necessitated a multifaceted rapid response by the scientific community, bringing researchers, health officials, and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with the etiological agent, the novel human coronavirus SARS-CoV-2. Work with infectious SARS-CoV-2 is currently restricted to high-containment laboratories, but material can be handled at a lower containment level after inactivation. Given the wide array of inactivation reagents that are being used in laboratories during this pandemic, it is vital that their effectiveness is thoroughly investigated. Here, we evaluated a total of 23 commercial reagents designed for clinical sample transportation, nucleic acid extraction, and virus inactivation for their ability to inactivate SARS-CoV-2, as well as seven other common chemicals, including detergents and fixatives. As part of this study, we have also tested five filtration matrices for their effectiveness at removing the cytotoxic elements of each reagent, permitting accurate determination of levels of infectious virus remaining following treatment. In addition to providing critical data informing inactivation methods and risk assessments for diagnostic and research laboratories working with SARS-CoV-2, these data provide a framework for other laboratories to validate their inactivation processes and to guide similar studies for other pathogens.

scholarly journals Inactivation analysis of SARS-CoV-2 by specimen transport media, nucleic acid extraction reagents, detergents and fixatives

2020 ◽  
Author(s):  
Stephen R. Welch ◽  
Katherine A. Davies ◽  
Hubert Buczkowski ◽  
Nipunadi Hettiarachchi ◽  
Nicole Green ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Infectious Virus ◽  
Clinical Sample ◽  
Accurate Determination ◽  
Acid Extraction ◽  
The Novel ◽  
Nucleic Acid Extraction ◽  
Human Coronavirus ◽  
Critical Data

AbstractThe COVID-19 pandemic has necessitated a rapid multi-faceted response by the scientific community, bringing researchers, health officials and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with the etiological agent, the novel human coronavirus SARS-CoV-2. Work with infectious SARS-CoV-2 is currently restricted to high-containment laboratories, but material can be handled at a lower containment level after inactivation. Given the wide array of inactivation reagents that are being used in laboratories during this pandemic, it is vital that their effectiveness is thoroughly investigated. Here, we evaluated a total of 23 commercial reagents designed for clinical sample transportation, nucleic acid extraction and virus inactivation for their ability to inactivate SARS-CoV-2, as well as seven other common chemicals including detergents and fixatives. As part of this study, we have also tested five filtration matrices for their effectiveness at removing the cytotoxic elements of each reagent, permitting accurate determination of levels of infectious virus remaining following treatment. In addition to providing critical data informing inactivation methods and risk assessments for diagnostic and research laboratories working with SARS-CoV-2, these data provide a framework for other laboratories to validate their inactivation processes and to guide similar studies for other pathogens.

Dual-recognition-based determination of ctDNA via the clamping function of peptide nucleic acid and terminal protection of small-molecule-linked DNA

The Analyst ◽  
2020 ◽  
Vol 145 (23) ◽  
pp. 7603-7608
Author(s):  
Chaohui Chen ◽  
Rongxiang He ◽  
Zhengtao Zhang ◽  
Yong Chen
Keyword(s):  
Nucleic Acid ◽  
Small Molecule ◽  
Peptide Nucleic Acid ◽  
Circulating Tumor Dna ◽  
Fluorescent Biosensor ◽  
Tumor Dna

A new dual-recognition fluorescent biosensor for circulating tumor DNA (ctDNA) detection has been developed, which combines the clamping function of peptide nucleic acid (PNA) and terminal protection of small-molecule-linked DNA (TPSMLD).

Toehold probe-based interrogation for haplotype phasing of long nucleic acid strands

2020 ◽  
Vol 12 (34) ◽  
pp. 4185-4190
Author(s):  
Xinyu Zhuang ◽  
Henson L. Lee Yu ◽  
I-Ming Hsing
Keyword(s):  
Nucleic Acid ◽  
Single Nucleotide Polymorphisms ◽  
Disease Risk ◽  
Accurate Determination ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Haplotype Phasing ◽  
Haplotype Phase

The arrangement of multiple single nucleotide polymorphisms (SNPs) in a gene, called a haplotype phase, is increasingly recognized as critical for accurate determination of disease risk and severity.

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