Electrochemical Determination of Hg2+Using Electrodes Modified with Peptide Nucleic Acid

2016 ◽  
Vol 163 (14) ◽  
pp. B667-B672 ◽  
Author(s):  
Agnieszka Bala ◽  
Krzysztof Zalewski ◽  
Łukasz Górski
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Electrochemical Determination

Preparation and determination of optical purity of γ-lysine modified peptide nucleic acid analogues

2012 ◽  
Vol 35 (3) ◽  
pp. 517-522 ◽  
Author(s):  
Hu Huang ◽  
Goon Ho Joe ◽  
Sung Rok Choi ◽  
Su Nam Kim ◽  
Yong Tae Kim ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Optical Purity ◽  
Nucleic Acid Analogues

Dual-recognition-based determination of ctDNA via the clamping function of peptide nucleic acid and terminal protection of small-molecule-linked DNA

The Analyst ◽  
2020 ◽  
Vol 145 (23) ◽  
pp. 7603-7608
Author(s):  
Chaohui Chen ◽  
Rongxiang He ◽  
Zhengtao Zhang ◽  
Yong Chen
Keyword(s):  
Nucleic Acid ◽  
Small Molecule ◽  
Peptide Nucleic Acid ◽  
Circulating Tumor Dna ◽  
Fluorescent Biosensor ◽  
Tumor Dna

A new dual-recognition fluorescent biosensor for circulating tumor DNA (ctDNA) detection has been developed, which combines the clamping function of peptide nucleic acid (PNA) and terminal protection of small-molecule-linked DNA (TPSMLD).

Synthesis and fluorescence properties of 3,6-diaminocarbazole-modified pyrrolidinyl peptide nucleic acid

RSC Advances ◽  
2016 ◽  
Vol 6 (78) ◽  
pp. 74314-74322 ◽  
Author(s):  
Aukkrapon Dangsopon ◽  
Nattawee Poomsuk ◽  
Khatcharin Siriwong ◽  
Tirayut Vilaivan ◽  
Chaturong Suparpprom
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Peptide Nucleic Acid ◽  
Fluorescence Properties

Diaminocarbazole-labeled acpcPNA as a self-reporting probe for determination of DNA sequences.

scholarly journals Peptide Nucleic Acid-Mediated PCR Clamping as a Useful Supplement in the Determination of Microbial Diversity

2000 ◽  
Vol 66 (2) ◽  
pp. 549-557 ◽  
Author(s):  
Friedrich von Wintzingerode ◽  
Olfert Landt ◽  
Angelika Ehrlich ◽  
Ulf B. Göbel
Keyword(s):  
Nucleic Acid ◽  
Microbial Diversity ◽  
Peptide Nucleic Acid ◽  
Pcr Amplification ◽  
Microbial Consortia ◽  
Phylogenetic Groups ◽  
Rdna Sequences ◽  
Gene Libraries ◽  
Ribosomal Dnas

ABSTRACT Peptide nucleic acid (PNA)-mediated PCR clamping (H. Ørum, P. E. Nielsen, M. Egholm, R. H. Berg, O. Buchardt, and C. Stanley, Nucleic Acids Res. 21:5332–5336, 1993) was introduced as a novel procedure to selectively amplify ribosomal DNAs (rDNAs) which are not frequently found in clone libraries generated by standard PCR from complex microbial consortia. Three different PNA molecules were used; two of these molecules (PNA-ALF and PNA-EUB353) overlapped with one of the amplification primers, whereas PNA-1114F hybridized to the middle of the amplified region. Thus, PCR clamping was achieved either by competitive binding between the PNA molecules and the forward or reverse primers (competitive clamping) or by hindering polymerase readthrough (elongation arrest). Gene libraries generated from mixed rDNA templates by using PCR clamping are enriched for clones that do not contain sequences homologous to the appropriate PNA oligomer. This effect of PCR clamping was exploited in the following two ways: (i) analysis of gene libraries generated by PCR clamping with PNA-ALF together with standard libraries reduced the number of clones which had to be analyzed to detect all of the different sequences present in an artificial rDNA mixture; and (ii) PCR clamping with PNA-EUB353 and PNA-1114F was used to selectively recover rDNA sequences which represented recently described phylogenetic groups (NKB19, TM6, cluster related to green nonsulfur bacteria) from an anaerobic, dechlorinating consortium described previously. We concluded that PCR clamping might be a useful supplement to standard PCR amplification in rDNA-based studies of microbial diversity and could be used to selectively recover members of undescribed phylogenetic clusters from complex microbial communities.

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