Tet system in the brain: Transgenic rats and lentiviral vectors approach

genesis ◽  
2009 ◽  
Vol 47 (4) ◽  
pp. 274-280 ◽  
Author(s):  
Witold Konopka ◽  
Kamila Duniec ◽  
Agata Klejman ◽  
Marcin Wawrzyniak ◽  
Dorota Owczarek ◽  
...  
Keyword(s):  
Lentiviral Vectors ◽  
Transgenic Rats ◽  
Tet System ◽  
The Brain

scholarly journals Identification of cell-type-specific promoters within the brain using lentiviral vectors

Gene Therapy ◽  
2007 ◽  
Vol 14 (7) ◽  
pp. 575-583 ◽  
Author(s):  
J P Chhatwal ◽  
S E Hammack ◽  
A M Jasnow ◽  
D G Rainnie ◽  
K J Ressler
Keyword(s):  
Lentiviral Vectors ◽  
Cell Type ◽  
Cell Type Specific ◽  
The Brain

Alterations in Blood Pressure and Heart Rate Variabililty in Transgenic Rats with Low Brain Angiotensinogen

Hypertension ◽  
2000 ◽  
Vol 36 (suppl_1) ◽  
pp. 727-727
Author(s):  
Ovidiu Baltatu ◽  
Ben J Janssen ◽  
Ralph Plehm ◽  
Detlev Ganten ◽  
Michael Bader
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Baroreflex Sensitivity ◽  
Circadian Variation ◽  
Ang Ii ◽  
Transgenic Rats ◽  
Short Term ◽  
Baroreflex Control ◽  
Sd Rats ◽  
The Brain

P191 The brain renin-angiotensin system (RAS) system may play a functional role in the long-term and short-term control of blood pressure (BPV) and heart rate variability (HRV). To study this we recorded in transgenic rats TGR(ASrAOGEN) with low brain angiotensinogen levels the 24-h variation of BP and HR during basal and hypertensive conditions, induced by a low-dose s.c. infusion of angiotensin II (Ang II, 100 ng/kg/min) for 7 days. Cardiovascular parameters were monitored by telemetry. Short-term BPV and HRV were evaluated by spectral analysis and as a measure of baroreflex sensitivity the transfer gain between the pressure and heart rate variations was calculated. During the Ang II infusion, in SD but not TGR(ASrAOGEN) rats, the 24-h rhythm of BP was inverted (5.8 ± 2 vs. -0.4 ± 1.8 mm Hg/group of day-night differences of BP, p< 0.05, respectively). In contrast, in both the SD and TGR(ASrAOGEN) rats, the 24-h HR rhythms remained unaltered and paralleled those of locomotor activity. The increase of systolic BP was significantly reduced in TGR(ASrAOGEN) in comparison to SD rats as previously described, while the HR was not altered in TGR(ASrAOGEN) nor in SD rats. The spectral index of baroreflex sensitivity (FFT gain between 0.3-0.6 Hz) was significantly higher in TGR(ASrAOGEN) than SD rats during control (0.71 ± 0.1 vs. 0.35 ± 0.06, p<0.05), but not during Ang II infusion (0.6 ± 0.07 vs. 0.4 ± 0.1, p>0.05). These results demonstrate that the brain RAS plays an important role in mediating the effects of Ang II on the circadian variation of BP. Furthermore these data are consistent with the view that the brain RAS modulates baroreflex control of HR in rats, with AII having an inhibitory role.

Aging and the brain renin-angiotensin system: insights from studies in transgenic rats.

2007 ◽  
Vol 74 (Suppl_1) ◽  
pp. S95-S95 ◽  
Author(s):  
D. I Diz ◽  
S. O Kasper ◽  
A. Sakima ◽  
C. M Ferrario
Keyword(s):  
Renin Angiotensin System ◽  
Transgenic Rats ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
The Brain

Differential Effects of Angiotensin II and Angiotensin-(1-7) at the Nucleus Tractus Solitarii of Transgenic Rats with Low Brain Angiotensinogen

Hypertension ◽  
2000 ◽  
Vol 36 (suppl_1) ◽  
pp. 700-700
Author(s):  
Aurea S Couto ◽  
Ovidiu Baltatu ◽  
Robson A S Santos ◽  
Detlev Ganten ◽  
Michael Bader ◽  
...  
Keyword(s):  
Heart Rate ◽  
Baroreflex Sensitivity ◽  
Renin Angiotensin System ◽  
Nucleus Tractus Solitarii ◽  
Ang Ii ◽  
Sprague Dawley ◽  
Transgenic Rats ◽  
Baroreflex Control ◽  
Sd Rats ◽  
The Brain

P42 The potential importance of permanent alteration of the brain renin-angiotensin system on angiotensin (Ang) II and Ang-(1-7) effects at the level of the nucleus tractus solitarii (NTS) was investigated in transgenic rats with a deficit in brain angiotensinogen production TGR(ASrAOGEN) (TGR). Ang II (10 pmol), Ang-(1-7) (10 pmol) or NaCl (0.9%/ 50 nl) were microinjected into the NTS of urethane-anesthetized TGR (n=28) and Sprague-Dawley (SD, n=22) rats. Mean arterial pressure (MAP) and heart rate (HR) were measured via a femoral artery catheter and the baroreflex control of heart rate was evaluated after increases in MAP induced by phenylephrine (baroreflex bradycardia). Ang II microinjections into the NTS of the TGR induced a higher decrease in MAP and HR (-37 ± 5 mmHg and -69 ± 12.5 beats/min, respectively) in comparison with SD rats (-18 ± 1 mmHg and -51 ± 11 beats/min, respectively). In contrast, changes after Ang-(1-7) microinjections into the NTS of TGR (-6 ± 1 mmHg and -13 ± 5 beats/min) were significantly smaller than that induced in SD (-11 ± 2 mmHg and -24 ± 8 beats/min.). The baroreflex sensitivity was accentuated in TGR in comparison to SD rats (0.69 ± 0.06 vs. 0.44 ± 0.03 ms/ mmHg). Ang II microinjection into the NTS produced similar attenuation in the baroreflex bradycardia in both SD (0.28 ± 0.07 vs. 0.5 ± 0.07 ms/ mmHg, before injection) and TGR (0.44 ± 0.1 vs. 0.82 ± 0.1ms/ mmHg, before injection). Ang-(1-7) microinjection elicited a facilitation of the baroreflex bradycardia in SD (0.62 ± 0.1 vs. 0.4 ± 0.03 ms/ mmHg, before injection). However in TGR, baroreflex bradycardia after Ang-(1-7) was not different from saline microinjection. These results indicate that a permanent inhibition of angiotensinogen synthesis in the brain can lead to a functional up-regulation of Ang II receptors. However, the putative Ang-(1-7) receptors seem to be desensitized in the NTS of these transgenic rats. The alterated baroreflex sensitivity, both before and after Ang microinjection, indicates the functionally relevant decrease in brain Ang in TGR and supports differential regulatory mechanisms for the effects of the two Ang peptides.

Expression of cytochrome P45011B1 mRNA in the brain of normal and hypertensive transgenic rats

1996 ◽  
Vol 733 (1) ◽  
pp. 73-82 ◽  
Author(s):  
Bettina Erdmann ◽  
Hellmut Gerst ◽  
Andrea Lippoldt ◽  
Hannes Bülow ◽  
Detlev Ganten ◽  
...  
Keyword(s):  
Transgenic Rats ◽  
The Brain

scholarly journals Neuronal‐derived extracellular vesicles are enriched in the brain and serum of HIV‐1 transgenic rats

2019 ◽  
Vol 9 (1) ◽  
pp. 1703249 ◽  
Author(s):  
Raghubendra Singh Dagur ◽  
Ke Liao ◽  
Susmita Sil ◽  
Fang Niu ◽  
Zhiqiang Sun ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Transgenic Rats ◽  
The Brain ◽  
Hiv 1

scholarly journals The brain renin-angiotensin system and cardiovascular responses to stress: insights from transgenic rats with low brain angiotensinogen

2012 ◽  
Vol 113 (12) ◽  
pp. 1929-1936 ◽  
Author(s):  
Amy C. Arnold ◽  
Atsushi Sakima ◽  
Sherry O. Kasper ◽  
Sherry Vinsant ◽  
Maria Antonia Garcia-Espinosa ◽  
...  
Keyword(s):  
Stress Responses ◽  
Renin Angiotensin System ◽  
Cardiovascular Responses ◽  
Ang Ii ◽  
Transgenic Rats ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Brain Peptides ◽  
The Impact ◽  
The Brain

The renin-angiotensin system (RAS) has been identified as an attractive target for the treatment of stress-induced cardiovascular disorders. The effects of angiotensin (ANG) peptides during stress responses likely result from an integration of actions by circulating peptides and brain peptides derived from neuronal and glial sources. The present review focuses on the contribution of endogenous brain ANG peptides to pathways involved in cardiovascular responses to stressors. During a variety of forms of stress, neuronal pathways in forebrain areas containing ANG II or ANG-(1–7) are activated to stimulate descending angiotensinergic pathways that increase sympathetic outflow to increase blood pressure. We provide evidence that glia-derived ANG peptides influence brain AT1 receptors. This appears to result in modulation of the responsiveness of the neuronal pathways activated during stressors that elevate circulating ANG peptides to activate brain pathways involving descending hypothalamic projections. It is well established that increased cardiovascular reactivity to stress is a significant predictor of hypertension and other cardiovascular diseases. This review highlights the importance of understanding the impact of RAS components from the circulation, neurons, and glia on the integration of cardiovascular responses to stressors.

Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors

2011 ◽  
pp. 117-135
Author(s):  
Séverine Ménoret ◽  
Séverine Remy ◽  
Laurent Tesson ◽  
Claire Usal ◽  
Anne-Laure Iscache ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Lentiviral Vectors ◽  
Transgenic Rats
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