Generation of Transgenic Rats Using Lentiviral Vectors

2014 ◽  
pp. 25-37 ◽  
Author(s):  
Holger M. Reichardt ◽  
Henrike J. Fischer
Keyword(s):  
Lentiviral Vectors ◽  
Transgenic Rats

Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors

2011 ◽  
pp. 117-135
Author(s):  
Séverine Ménoret ◽  
Séverine Remy ◽  
Laurent Tesson ◽  
Claire Usal ◽  
Anne-Laure Iscache ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Lentiviral Vectors ◽  
Transgenic Rats

The Use of Lentiviral Vectors to Obtain Transgenic Rats

2009 ◽  
pp. 109-125 ◽  
Author(s):  
Séverine Remy ◽  
Tuan Huy Nguyen ◽  
Séverine Ménoret ◽  
Laurent Tesson ◽  
Claire Usal ◽  
...  
Keyword(s):  
Lentiviral Vectors ◽  
Transgenic Rats

300 DEVELOPMENT OF TETRACYCLINE-REGULATED GREEN FLUORESCENT PROTEIN EXPRESSING TRANSGENIC RATS BY LENTIVIRAL VECTOR

2009 ◽  
Vol 21 (1) ◽  
pp. 247
Author(s):  
C. Agca ◽  
A. Chan ◽  
Y. Agca
Keyword(s):  
Green Fluorescent Protein ◽  
Blot Analysis ◽  
Fluorescent Protein ◽  
Lentiviral Vector ◽  
Lentiviral Vectors ◽  
Sprague Dawley ◽  
Transgenic Rats ◽  
Gfp Gene ◽  
Green Fluorescent ◽  
Kidney Liver

Although various methodologies have been available for genetic modification in mouse, such genomic tools are very limited in rats. The aim of the present study was to take advantage of lentiviral gene delivery to develop effective inducible germline transgenic rats. A tetracycline inducible system was designed to control the expression of the green fluorescent protein (GFP) gene. The GFP gene was constructed downstream of tetracycline response element under the control of a mini cytomegalovirus promoter. Additionally, rtTA2s-Ms was constructed at the 3 prime terminus of GFP under the control of an ubiquitin promoter. The resulted construct was named pLV-Tet-GFP. The pLV-Tet-GFP lentiviral vectors were injected into perivitelline space of 115 Sprague-Dawley rat zygotes. Of those 115 zygotes injected, 93 zygotes were transferred into 4 surrogate mothers and 32 live pups were obtained. The presence of transgene in Fo generation was first detected by PCR and subsequently Southern blot analysis in 25 out of 32 pups (78%). A varying level of GFP expression was observed in the 14 Fo pups after feeding them with 2 g L–1 of tetracycline via drinking water for 4 days. Northern blot analysis was performed in the subsequent generations to determine spatial (brain, kidney, liver, and lung tissues) and temporal (day 0, day 4, day 11, and day 30) expression. The results showed expression intensity as being highest in kidney, brain, lung, and liver. There was little expression detected on day 11 and 30. This study suggests that lentiviral vectors can be used to deliver inducible system quite efficiently in rats. The authors thank Dr. Carlos Lois for providing the lentiviral backbone, Dr. W. Hillen for providing the rtTA2s-Ms fragment, and GuoFu Fang for developing the inducible lentiviral vector.

Tet system in the brain: Transgenic rats and lentiviral vectors approach

genesis ◽  
2009 ◽  
Vol 47 (4) ◽  
pp. 274-280 ◽  
Author(s):  
Witold Konopka ◽  
Kamila Duniec ◽  
Agata Klejman ◽  
Marcin Wawrzyniak ◽  
Dorota Owczarek ◽  
...  
Keyword(s):  
Lentiviral Vectors ◽  
Transgenic Rats ◽  
Tet System ◽  
The Brain

Potential of Motor Neurons in the Nucleus Ambiguus ∼ Utilizing Nestin-EGFP Transgenic Rats

2007 ◽  
Vol 58 (2) ◽  
pp. 193-193
Author(s):  
T. Takaoka ◽  
A. Shiotani ◽  
K. Saito ◽  
M. Tomifuji ◽  
Y. Mori ◽  
...  
Keyword(s):  
Motor Neurons ◽  
Nucleus Ambiguus ◽  
Transgenic Rats

Impaired Estrogen Sensitivity in Bone by Inhibiting Both Estrogen Receptor a and b Pathways*

2020 ◽  
Author(s):  
Lungwani Muungo
Keyword(s):  
Bone Mineral Density ◽  
Estrogen Receptor ◽  
Bone Loss ◽  
Bone Turnover ◽  
Bone Mineral ◽  
Female Rats ◽  
Wild Type ◽  
Mineral Density ◽  
Transgenic Rats ◽  
Estrogen Sensitivity

Although it is well established that estrogen deficiencycauses osteoporosis among the postmenopausalwomen, the involvement of estrogen receptor (ER) in itspathogenesis still remains uncertain. In the presentstudy, we have generated rats harboring a dominantnegative ERa, which inhibits the actions of not only ERabut also recently identified ERb. Contrary to our expectation,the bone mineral density (BMD) of the resultingtransgenic female rats was maintained at the same levelwith that of the wild-type littermates when sham-operated.In addition, ovariectomy-induced bone loss wasobserved almost equally in both groups. Strikingly, however,the BMD of the transgenic female rats, after ovariectomized,remained decreased even if 17b-estradiol(E2) was administrated, whereas, in contrast, the decreaseof littermate BMD was completely prevented byE2. Moreover, bone histomorphometrical analysis ofovariectomized transgenic rats revealed that the higherrates of bone turnover still remained after treatmentwith E2. These results demonstrate that the preventionfrom the ovariectomy-induced bone loss by estrogen ismediated by ER pathways and that the maintenanceof BMD before ovariectomy might be compensatedby other mechanisms distinct from ERa and ERbpathways.

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