Lentiviral Vectors Mediate Nonimmunosuppressive Rapamycin Analog-Induced Production of Secreted Therapeutic Factors in the Brain: Regulation at the Level of Transcription and Exocytosis

2008 ◽  
Vol 19 (2) ◽  
pp. 167-178 ◽  
Author(s):  
Roland Vogel ◽  
Hamid Mammeri ◽  
Jacques Mallet
Keyword(s):  
Lentiviral Vectors ◽  
Therapeutic Factors ◽  
The Brain

scholarly journals Identification of cell-type-specific promoters within the brain using lentiviral vectors

Gene Therapy ◽  
2007 ◽  
Vol 14 (7) ◽  
pp. 575-583 ◽  
Author(s):  
J P Chhatwal ◽  
S E Hammack ◽  
A M Jasnow ◽  
D G Rainnie ◽  
K J Ressler
Keyword(s):  
Lentiviral Vectors ◽  
Cell Type ◽  
Cell Type Specific ◽  
The Brain

Effective Transduction of Brain Neurons with Lentiviral Vectors Purified by Ion-Exchange Chromatography

2020 ◽  
Vol 36 (5) ◽  
pp. 89-97
Author(s):  
D.A. Lanshakov
Keyword(s):  
Gene Therapy ◽  
Ion Exchange ◽  
High Titer ◽  
Lentiviral Vectors ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
In Vivo Studies ◽  
Modern Biology ◽  
The Brain

The development of methods for purification viral vectors for gene therapy is one of the most important and urgent problems of modern biology and medicine. Recently, drugs that carry cerebral neurotrophic factors, such as BDNF, have become increasingly popular. However, viral drugs for gene therapy should meet certain requirements, including high titer and applicability for in vivo studies. At the same time, the creation of such vectors requires cost-effective, inexpensive and affordable methods for standard laboratories. This study compares various methods for purification of lentiviral vectors encoding the brain neurotrophic factor, BDNF. The highest titer (1.12 ∙ 109/mL) was obtained via PEG 6 000 precipitation followed by anion-exchange chromatography on two columns of sorbents containing quaternary ammonium groups. Abnormal aggregates of transduced neurons were detected after lentiviruses purified only by PEG precipitation were injected into the brain of a newborn rat. This fact confirms the necessity of the proposed additional chromatographic purification stage. lentivirus, BDNF, ion exchange chromatography, gene therapy, PEG This work was financially supported by budgetary funding project no. 0259-2019-0003-C-01.

Evidence for disease-regulated transgene expression in the brain with use of lentiviral vectors

2006 ◽  
Vol 84 (1) ◽  
pp. 58-67 ◽  
Author(s):  
Johan Jakobsson ◽  
Nina Rosenqvist ◽  
Karl Mårild ◽  
Denes v. Agoston ◽  
Cecilia Lundberg
Keyword(s):  
Transgene Expression ◽  
Lentiviral Vectors ◽  
The Brain

Tet system in the brain: Transgenic rats and lentiviral vectors approach

genesis ◽  
2009 ◽  
Vol 47 (4) ◽  
pp. 274-280 ◽  
Author(s):  
Witold Konopka ◽  
Kamila Duniec ◽  
Agata Klejman ◽  
Marcin Wawrzyniak ◽  
Dorota Owczarek ◽  
...  
Keyword(s):  
Lentiviral Vectors ◽  
Transgenic Rats ◽  
Tet System ◽  
The Brain
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