scholarly journals Expression of hedgehog signalling components in adult mouse testis

2006 ◽  
Vol 235 (11) ◽  
pp. 3063-3070 ◽  
Author(s):  
Anette Szczepny ◽  
Gary R. Hime ◽  
Kate L. Loveland
Keyword(s):  
Adult Mouse ◽  
Mouse Testis ◽  
Hedgehog Signalling

247. Expression of components of the hedgehog signalling pathway during murine spermatogenesis

2005 ◽  
Vol 17 (9) ◽  
pp. 98 ◽  
Author(s):  
A. Szczepny ◽  
D. A. Jans ◽  
G. Hime ◽  
K. L. Loveland
Keyword(s):  
Adult Mouse ◽  
Transmembrane Protein ◽  
Signalling Pathway ◽  
Negative Regulator ◽  
Mouse Testis ◽  
Hedgehog Signalling ◽  
Hedgehog Signalling Pathway ◽  
Sites Of Action ◽  
Lower Expression

Hedgehog (Hh) signalling is best known for its involvement in regulating patterning, driving cell proliferation, promoting cell survival and directing differentiation during embryonic development.1 The role that Hedgehog signalling plays in the testis is not yet clearly defined, though deletion of one Hedgehog ligand, Dhh, leads to male infertility. The Gli family of zinc finger TFs, consisting of Gli1, Gli2 and Gli3, are mediators of the Hh signalling cascade in vertebrates. We have previously shown that the mRNA transcripts encoding all three Glis in the adult mouse testis are expressed highly in spermatogonia, spermatocytes and to a lower extent in the round spermatids. To understand the potential sites of action of Hh proteins in spermatogenesis, we have extended our analysis to other genes involved in the Hh signalling pathway in the adult mouse testis. Using in situ hybridization, Patched2, a transmembrane receptor for Hh, was detected in spermatogonia and spermatocytes, with an apparently lower expression in the round spermatids. The mRNA of Smoothened, another transmembrane protein which forms a membrane receptor complex with Patched, is highly expressed in spermatogonia and spermatocytes, again showing lower expression in round spermatids and interstitial cells. Fused, a positive regulator of Hh signalling, is highly expressed in spermatogonia and spermatocytes with slightly lower expression in round spermatids. SuFu is a negative regulator of Hh signalling, known to repress Gli1 function in part by tethering it in the cytoplasm. The mRNA encoding SuFu is absent from spermatogonia, detected in spermatocytes and persists in round spermatids where its expression appears highest, suggesting that the SuFu protein may be acting to switch off Hh signalling at that stage of spermatogenesis. Overall, the regulated expression pattern of these genes in the adult mouse testis suggests a role for Hh signalling in the regulation of spermatogenesis. (1)Ruiz i Altaba A. (1999) Trends Genet. 15(10), 418–425.

scholarly journals Expression of Wsb2 in the developing and adult mouse testis

Reproduction ◽  
2007 ◽  
Vol 133 (4) ◽  
pp. 753-761 ◽  
Author(s):  
M A Sarraj ◽  
P J McClive ◽  
A Szczepny ◽  
H Daggag ◽  
K L Loveland ◽  
...  
Keyword(s):  
Real Time ◽  
Germ Cells ◽  
Adult Mouse ◽  
Gonad Development ◽  
Gonadal Development ◽  
Mouse Testis ◽  
Hedgehog Signalling ◽  
Real Time Analysis ◽  
Female Specific ◽  
Male Specific

We present a detailed study of the expression pattern of WD repeat and SOCS box-containing 2 (Wsb2) in mouse embryonic and adult gonads. Wsb2 was previously identified in a differential screen aimed at identifying the genes involved in male- and female-specific gonadal development. Wsb2 expression was analysed during mouse gonadogenesis by real-time PCR, whole-mount and section in situ hybridisation and immunofluorescence. Wsb2 mRNA expression was initially detected in gonads of both sexes from 11.5 days post coitum (dpc) until 12.0 dpc. By 12.5 dpc and thereafter, Wsb2 expression rapidly decreased in the female, while persisting in the male gonads. In foetal, newborn and juvenile testes, Wsb2 mRNA and protein were readily detected in the seminiferous cords within both Sertoli and germ cells. Wsb2 mRNA was present in spermatogonia, spermatocytes and in Sertoli cells of the adult mouse testis. The differential expression of Wsb2 in male versus female embryonic gonads suggests some male-specific role in gonad development, and its expression in the first wave of spermatogenesis indicates a role in germ cells. Real-time analysis of adult mouse testis tubules cultured in the presence of the Hedgehog signalling inhibitor, cyclopamine, showed a downregulation of Wsb2 mRNA after treatment which suggests that Wsb2 may be a target of Hedgehog signalling.

scholarly journals Fuzzy modeling reveals a dynamic self-sustaining network of the GLI transcription factors controlling important metabolic regulators in adult mouse hepatocytes

2015 ◽  
Vol 11 (8) ◽  
pp. 2190-2197 ◽  
Author(s):  
Wolfgang Schmidt-Heck ◽  
Madlen Matz-Soja ◽  
Susanne Aleithe ◽  
Eugenia Marbach ◽  
Reinhard Guthke ◽  
...  
Keyword(s):  
Fuzzy Logic ◽  
Transcription Factors ◽  
Regulatory Network ◽  
Adult Mouse ◽  
Dynamic Features ◽  
Hedgehog Signalling ◽  
Gli Transcription Factors ◽  
Mouse Hepatocytes ◽  
Metabolic Regulators ◽  
Modelling Approach

The Hedgehog signalling-driven Gli transcription factors in hepatocytes form a regulatory network identified by a fuzzy-logic modelling approach. The network explains dynamic features important for hepatocyte function and fate.

Differential RA responsiveness among subsets of mouse late progenitor spermatogonia

Reproduction ◽  
2021 ◽  
Author(s):  
Shinnosuke Suzuki ◽  
John R. McCarrey ◽  
Brian P Hermann
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Single Cell ◽  
Adult Mouse ◽  
Retinoic Acid Receptor ◽  
Mouse Testis ◽  
Rna Seq ◽  
Reporter Expression ◽  
Acid Receptor ◽  
Whole Mount

Initiation of spermatogonial differentiation in the mouse testis begins with the response to retinoic acid (RA) characterized by activation of KIT and STRA8 expression. In the adult, spermatogonial differentiation is spatiotemporally coordinated by a pulse of RA every 8.6 days that is localized to stages VII-VIII of the seminiferous epithelial cycle. Dogmatically, progenitor spermatogonia that express retinoic acid receptor gamma (RARG) at these stages will differentiate in response to RA, but this has yet to be tested functionally. Previous single-cell RNA-seq data identified phenotypically and functionally distinct subsets of spermatogonial stem cells (SSCs) and progenitor spermatogonia, where late progenitor spermatogonia were defined by expression of RARG and Dppa3. Here, we found late progenitor spermatogonia (RARGhigh KIT-) were further divisible into two subpopulations based on Dppa3 reporter expression (Dppa3-ECFP or Dppa3-EGFP) and were observed across all stages of the seminiferous epithelial cycle. However, nearly all Dppa3+ spermatogonia were differentiating (KIT+) late in the seminiferous epithelial cycle (stages X-XII), while Dppa3- late progenitors remained abundant, suggesting that Dppa3+ and Dppa3- late progenitors differentially responded to RA. Following acute RA treatment (2-4hr), significantly more Dppa3+ late progenitors induced KIT, including at the midpoint of the cycle (stages VI-IX), than Dppa3- late progenitors. Subsequently, single-cell analyses indicated a subset of Dppa3+ late progenitors expressed higher levels of Rxra, which we confirmed by RXRA whole-mount immunostaining. Together, these results indicate RARG alone is insufficient to initiate a spermatogonial response to RA in the adult mouse testis and suggest differential RXRA expression may discriminate responding cells.

Correlation between expression of CatSper1,2 and sperm parameters in the gamma irradiated adult mouse testis

2019 ◽  
Vol 95 (6) ◽  
pp. 691-696 ◽  
Author(s):  
Shabnam Mohammadi ◽  
Mojtaba Kianmehr ◽  
Maryam Mohammadi ◽  
Zahra Fahimian ◽  
Elham Karimimanesh ◽  
...  
Keyword(s):  
Adult Mouse ◽  
Mouse Testis ◽  
Sperm Parameters ◽  
Gamma Irradiated

scholarly journals IDENTIFICATION OF A NOVEL SOMATIC STEM CELL POPULATION IN ADULT MOUSE TESTIS INVOLVED IN TISSUE HOMEOSTASIS AND REGENERATION

2020 ◽  
Vol 114 (3) ◽  
pp. e397
Author(s):  
Adrienne N. Shami ◽  
Yu-chi Shen ◽  
Hailey Larose ◽  
Lindsay Moritz ◽  
Gabriel Manske ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Population ◽  
Adult Mouse ◽  
Tissue Homeostasis ◽  
Mouse Testis ◽  
Stem Cell Population ◽  
Somatic Stem Cell

Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis

2011 ◽  
Vol 112 (4) ◽  
pp. 1009-1021 ◽  
Author(s):  
Shanshan Zhang ◽  
Junwei Sun ◽  
Shaohui Pan ◽  
Haijing Zhu ◽  
Long Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vitamin A ◽  
Adult Mouse ◽  
Mouse Testis ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Male Germline ◽  
Male Germline Stem Cells

scholarly journals DNA analysis and sorting of viable mouse testis cells.

1981 ◽  
Vol 29 (6) ◽  
pp. 738-746 ◽  
Author(s):  
W M Grogan ◽  
W F Farnham ◽  
J M Sabau
Keyword(s):  
Dna Content ◽  
Adult Mouse ◽  
Dna Analysis ◽  
Cell Types ◽  
Mouse Testis ◽  
Spermatogenic Cells ◽  
Hoechst 33342 ◽  
Cell Sorter ◽  
Pachytene Spermatocytes

The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells were enriched to 90%, a 10-fold purification. The capability for viable sorting of most testis cell types to homogeneity in numbers suitable for many biochemical applications is demonstrated.

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