Ultrastructural changes induced by HCG in the Leydig cell of the adult mouse testis

1970 ◽  
Vol 112 (3) ◽  
pp. 363-370 ◽  
Author(s):  
J. Russo ◽  
F. L. Sacerdote
Keyword(s):  
Leydig Cell ◽  
Adult Mouse ◽  
Mouse Testis ◽  
Ultrastructural Changes

scholarly journals Slit/Robo signaling regulates Leydig cell steroidogenesis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Emmanuelle Martinot ◽  
Derek Boerboom
Keyword(s):  
Cancer Progression ◽  
Leydig Cell ◽  
Leydig Cells ◽  
Adult Mouse ◽  
Primary Cultures ◽  
Mouse Testis ◽  
Mrna Levels ◽  
Pathologic Processes ◽  
In Vitro Treatment

Abstract Background First identified as a regulator of neuronal axon guidance, Slit/Robo signaling has since been implicated in additional physiologic and pathologic processes, such as angiogenesis, organogenesis and cancer progression. However, its roles in the regulation of testis function have been little explored. Methods Immunohistochemistry and RT-qPCR analyses were performed to detect the expression of Slit/Robo signaling effectors in the adult mouse testis. To identify the roles and mechanisms of Slit/Robo signaling in the regulation of steroidogenesis, RT-qPCR, immunoblotting and hormone measurements were carried out using Leydig cells (primary cultures and the MA10 cell line) treated with exogenous SLIT ligands, and testes from Robo1-null mice. Results Slit1, -2 and -3 and Robo1 and -2 expression was detected in the adult mouse testis, particularly in Leydig cells. In vitro treatment of Leydig cells with exogenous SLIT ligands led to a decrease in the expression of the steroidogenic genes Star, Cyp11a1, and Cyp17a1. SLIT2 treatment decreased the phosphorylation of the key steroidogenic gene regulator CREB, possibly in part by suppressing AKT activity. Furthermore, SLIT2 treatment reduced the responsiveness of MA10 cells to luteinizing hormone by decreasing the expression of Lhcgr. Consistent with these in vitro results, an increase in testicular Star mRNA levels and intra-testicular testosterone concentrations were found in Robo1-null mice. Finally, we showed that the expression of the Slit and Robo genes in Leydig cells is enhanced by testosterone treatment in vitro, by an AR-independent mechanism. Conclusion Taken together, these results suggest that Slit/Robo signaling represents a novel mechanism that regulates Leydig cell steroidogenesis. It may act in an autocrine/paracrine manner to mediate negative feedback by testosterone on its own synthesis.

scholarly journals Sertoli Cells Maintain Leydig Cell Number and Peritubular Myoid Cell Activity in the Adult Mouse Testis

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105687 ◽  
Author(s):  
Diane Rebourcet ◽  
Peter J. O’Shaughnessy ◽  
Ana Monteiro ◽  
Laura Milne ◽  
Lyndsey Cruickshanks ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Sertoli Cells ◽  
Adult Mouse ◽  
Cell Activity ◽  
Cell Number ◽  
Mouse Testis ◽  
Myoid Cell ◽  
Peritubular Myoid Cell

scholarly journals Leydig cell genes change their expression and association with polysomes in a stage-specific manner in the adult mouse testis†,‡

2018 ◽  
Vol 98 (5) ◽  
pp. 722-738 ◽  
Author(s):  
Estela J Jauregui ◽  
Debra Mitchell ◽  
Savanna M Garza ◽  
Traci Topping ◽  
Cathryn A Hogarth ◽  
...  
Keyword(s):  
Germ Cell ◽  
Leydig Cell ◽  
Leydig Cells ◽  
Adult Mouse ◽  
Cell Function ◽  
Cell Development ◽  
Seminiferous Epithelium ◽  
Mouse Testis ◽  
Germ Cell Development ◽  
Specific Manner

Abstract Spermatogenesis in mammals occurs in a very highly organized manner within the seminiferous epithelium regulated by different cell types in the testis. Testosterone produced by Leydig cells regulates blood–testis barrier formation, meiosis, spermiogenesis, and spermiation. However, it is unknown whether Leydig cell function changes with the different stages of the seminiferous epithelium. This study utilized the WIN 18,446 and retinoic acid (RA) treatment regime combined with the RiboTag mouse methodology to synchronize male germ cell development and allow for the in vivo mapping of the Leydig cell translatome across the different stages of one cycle of the seminiferous epithelium. Using microarrays analysis, we identified 11 Leydig cell-enriched genes that were expressed in stage-specific manner such as the glucocorticoid synthesis and transport genes, Cyp21a1 and Serpina6. In addition, there were nine Leydig cell transcripts that change their association with polysomes in correlation with the different stages of the spermatogenic cycle including Egr1. Interestingly, the signal intensity of EGR1 and CYP21 varied among Leydig cells in the adult asynchronous testis. However, testosterone levels across the different stages of germ cell development did not cycle. These data show, for the first time, that Leydig cell gene expression changes in a stage-specific manner during the cycle of the seminiferous epithelium and indicate that a heterogeneous Leydig cell population exists in the adult mouse testis.

scholarly journals Sertoli Cell Number Defines and Predicts Germ and Leydig Cell Population Sizes in the Adult Mouse Testis

Endocrinology ◽  
2017 ◽  
Vol 158 (9) ◽  
pp. 2955-2969 ◽  
Author(s):  
Diane Rebourcet ◽  
Annalucia Darbey ◽  
Ana Monteiro ◽  
Ugo Soffientini ◽  
Yi Ting Tsai ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Cell Population ◽  
Leydig Cell ◽  
Adult Mouse ◽  
Cell Number ◽  
Mouse Testis ◽  
Population Sizes

Differential RA responsiveness among subsets of mouse late progenitor spermatogonia

Reproduction ◽  
2021 ◽  
Author(s):  
Shinnosuke Suzuki ◽  
John R. McCarrey ◽  
Brian P Hermann
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Single Cell ◽  
Adult Mouse ◽  
Retinoic Acid Receptor ◽  
Mouse Testis ◽  
Rna Seq ◽  
Reporter Expression ◽  
Acid Receptor ◽  
Whole Mount

Initiation of spermatogonial differentiation in the mouse testis begins with the response to retinoic acid (RA) characterized by activation of KIT and STRA8 expression. In the adult, spermatogonial differentiation is spatiotemporally coordinated by a pulse of RA every 8.6 days that is localized to stages VII-VIII of the seminiferous epithelial cycle. Dogmatically, progenitor spermatogonia that express retinoic acid receptor gamma (RARG) at these stages will differentiate in response to RA, but this has yet to be tested functionally. Previous single-cell RNA-seq data identified phenotypically and functionally distinct subsets of spermatogonial stem cells (SSCs) and progenitor spermatogonia, where late progenitor spermatogonia were defined by expression of RARG and Dppa3. Here, we found late progenitor spermatogonia (RARGhigh KIT-) were further divisible into two subpopulations based on Dppa3 reporter expression (Dppa3-ECFP or Dppa3-EGFP) and were observed across all stages of the seminiferous epithelial cycle. However, nearly all Dppa3+ spermatogonia were differentiating (KIT+) late in the seminiferous epithelial cycle (stages X-XII), while Dppa3- late progenitors remained abundant, suggesting that Dppa3+ and Dppa3- late progenitors differentially responded to RA. Following acute RA treatment (2-4hr), significantly more Dppa3+ late progenitors induced KIT, including at the midpoint of the cycle (stages VI-IX), than Dppa3- late progenitors. Subsequently, single-cell analyses indicated a subset of Dppa3+ late progenitors expressed higher levels of Rxra, which we confirmed by RXRA whole-mount immunostaining. Together, these results indicate RARG alone is insufficient to initiate a spermatogonial response to RA in the adult mouse testis and suggest differential RXRA expression may discriminate responding cells.

Correlation between expression of CatSper1,2 and sperm parameters in the gamma irradiated adult mouse testis

2019 ◽  
Vol 95 (6) ◽  
pp. 691-696 ◽  
Author(s):  
Shabnam Mohammadi ◽  
Mojtaba Kianmehr ◽  
Maryam Mohammadi ◽  
Zahra Fahimian ◽  
Elham Karimimanesh ◽  
...  
Keyword(s):  
Adult Mouse ◽  
Mouse Testis ◽  
Sperm Parameters ◽  
Gamma Irradiated

scholarly journals IDENTIFICATION OF A NOVEL SOMATIC STEM CELL POPULATION IN ADULT MOUSE TESTIS INVOLVED IN TISSUE HOMEOSTASIS AND REGENERATION

2020 ◽  
Vol 114 (3) ◽  
pp. e397
Author(s):  
Adrienne N. Shami ◽  
Yu-chi Shen ◽  
Hailey Larose ◽  
Lindsay Moritz ◽  
Gabriel Manske ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Population ◽  
Adult Mouse ◽  
Tissue Homeostasis ◽  
Mouse Testis ◽  
Stem Cell Population ◽  
Somatic Stem Cell

Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis

2011 ◽  
Vol 112 (4) ◽  
pp. 1009-1021 ◽  
Author(s):  
Shanshan Zhang ◽  
Junwei Sun ◽  
Shaohui Pan ◽  
Haijing Zhu ◽  
Long Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vitamin A ◽  
Adult Mouse ◽  
Mouse Testis ◽  
Germline Stem Cells ◽  
Self Renewal ◽  
Male Germline ◽  
Male Germline Stem Cells

scholarly journals DNA analysis and sorting of viable mouse testis cells.

1981 ◽  
Vol 29 (6) ◽  
pp. 738-746 ◽  
Author(s):  
W M Grogan ◽  
W F Farnham ◽  
J M Sabau
Keyword(s):  
Dna Content ◽  
Adult Mouse ◽  
Dna Analysis ◽  
Cell Types ◽  
Mouse Testis ◽  
Spermatogenic Cells ◽  
Hoechst 33342 ◽  
Cell Sorter ◽  
Pachytene Spermatocytes

The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells were enriched to 90%, a 10-fold purification. The capability for viable sorting of most testis cell types to homogeneity in numbers suitable for many biochemical applications is demonstrated.

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