Detection of high-risk human papillomavirus (HPV) DNA by Hybrid Capture II in women referred due to atypical glandular cells in the primary screening

2004 ◽  
Vol 31 (1) ◽  
pp. 19-22 ◽  
Author(s):  
Eliane R.Z.M. Oliveira ◽  
Sophie F.M. Derchain ◽  
Silvia H. Rabelo-Santos ◽  
Maria Cristina A. Westin ◽  
Luiz Carlos Zeferino ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Primary Screening ◽  
Glandular Cells ◽  
Atypical Glandular Cells

scholarly journals Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers.

2006 ◽  
Vol 53 (3) ◽  
pp. 603-607 ◽  
Author(s):  
Slawa Szostek ◽  
Malgorzata Klimek ◽  
Barbara Zawilinska ◽  
Janusz Rys ◽  
Jolanta Kope ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Consensus Primer ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Cervical Smears ◽  
Capture Assay ◽  
Cervical Cell ◽  
Target Dna ◽  
Primer Sets

The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R primers. Target DNA from the HPV L1 region was amplified by SPF10 primer set and home-PCR with MY09/MY11 primers. The presence of HPV DNA in cervical smears was detected by SPF10 (in 72% of cases), MY09/MY11 (58%), hybrid capture (55%) and pU-1M/pU-2R (39%). Results obtained with the SPF10 and MY09/MY11 consensus primer sets as well as hybrid capture and pU-1M/pU-2R specific for high-risk types differed significantly (chi2, P

High-risk human papillomavirus DNA testing: A marker for atypical glandular cells

2006 ◽  
Vol 34 (3) ◽  
pp. 235-239 ◽  
Author(s):  
Anjali Saqi ◽  
Prabodh K. Gupta ◽  
Maria Erroll ◽  
Anamaria Babiac ◽  
Dorothy Blackmun ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Dna Testing ◽  
Glandular Cells ◽  
Atypical Glandular Cells ◽  
Papillomavirus Dna

scholarly journals Evaluation of a liquid bead array system for high-risk human papillomavirus detection and genotyping in comparison with Hybrid Capture II, DNA chip and sequencing methods

2011 ◽  
Vol 60 (2) ◽  
pp. 162-171 ◽  
Author(s):  
Eun Jung Cho ◽  
Jin Hwan Do ◽  
Yoon Sun Kim ◽  
Sumi Bae ◽  
Woong Shick Ahn
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Intraepithelial Neoplasia ◽  
Dna Chip ◽  
Detection Methods ◽  
Specific Information ◽  
Perfect Agreement ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Genotype Information

Since persistent infection with high-risk human papillomavirus (HPV) is a known cause of high-grade cervical intraepithelial neoplasia and cervical cancer, several HPV DNA detection methods have been developed during the last decade. The Hybrid Capture II (HCII) assay, which allows detection of 13 high-risk HPVs, has been well validated; however, it does not provide any genotype-specific information. The oncogenic activity of HPV is dependent on its genotype. The prophylactic effects of HPV vaccines are based on L1 virus-like particles and are limited mainly to infections corresponding to the HPV type used to develop the immunogen. Therefore, accurate detection and genotyping are important for treatment as well as screening. A newly developed HPV genotyping system using a liquid bead array was evaluated with 286 cervical samples and the results were compared to two commercially available methods, i.e. the HCII and HPV DNA chip assays, and sequencing. The sensitivity for detection of high-risk HPV was 85.3 % (HCII), 94.7 % (DNA chip) and 99.0 % (liquid bead array). The liquid bead array showed almost perfect agreement (κ=0.95) with genotype information confirmed by sequencing, while substantial agreement (κ=0.8) was observed between DNA chip and sequencing. Furthermore, the liquid bead array had superior detection of 26 HPVs (16 high-risk and 10 low-risk types) and has proven to be as accurate as sequencing in identifying individual HPV types, even in cases with multiple HPV infections.

Significance of High-Risk Human Papillomavirus Testing for Atypical Glandular Cells on Cervical Cytology

2018 ◽  
Vol 62 (5-6) ◽  
pp. 405-410 ◽  
Author(s):  
Hiroaki Komatsu ◽  
Tetsuro Oishi ◽  
Daiken Osaku ◽  
Mayumi Sawada ◽  
Akiko Kudoh ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Intraepithelial Neoplasia ◽  
Adenocarcinoma In Situ ◽  
Diagnostic Significance ◽  
Predictive Values ◽  
Squamous Cells ◽  
Glandular Cells ◽  
Atypical Glandular Cells

Objectives: The aim of this study was to evaluate the diagnostic significance of high-risk human papillomavirus (hrHPV) testing for managing women with atypical glandular cells (AGC) and to explore the distribution of hrHPV genotypes. Methods: We analyzed cytologic and histopathologic diagnoses in patients referred to our institution due to AGC or atypical squamous cells of undetermined significance (ASC-US). All patients underwent hrHPV testing and genotyping, and positive (PPV) and negative predictive values (NPV) for cervical intraepithelial neoplasia (CIN) 2 or worse [CIN2+/adenocarcinoma in situ (AIS)+] were calculated. Results: Among 41 cases previously diagnosed with AGC, 22 (53%) were classified as CIN2+ (2 squamous cell carcinomas), whereas only 2 were AIS or adenocarcinoma. Twenty-seven (65.8%) cases in the AGC group were hrHPV positive. The most frequent genotypes in both the ASC-US and AGC groups were HPV16 and HPV52. The PPV of hrHPV testing for CIN2+/AIS+ was significantly higher in the AGC than in the ASC-US group (74.1 vs. 35.0%; p = 0.0005). The NPV for CIN2+/AIS+ was significantly lower in the AGC than in the ASC-US group (74.4 vs. 100%; p = 0.0441). Conclusion: In patients with AGC, both glandular and squamous lesions must be monitored. hrHPV testing is useful for detecting CIN2+/AIS+ in AGC.

Atypical glandular cells (AGC): ThinPrep imaging system (TIS), manual screening (MS), and correlation with Hybrid Capture 2 (HC2) HPV DNA testing

2009 ◽  
pp. n/a-n/a
Author(s):  
Anthony N. Sireci ◽  
John P. Crapanzano ◽  
Mahesh Mansukhani ◽  
Thomas Wright ◽  
Anamaria Babiac ◽  
...  
Keyword(s):  
Imaging System ◽  
Dna Testing ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Hybrid Capture 2 ◽  
Glandular Cells ◽  
Atypical Glandular Cells
Sign in / Sign up

Export Citation Format

Share Document