Flow cytometric immunophenotyping including Bcl-2 detection on fine needle aspirates in the diagnosis of reactive lymphadenopathy and non-Hodgkin's lymphoma

2005 ◽  
Vol 64B (1) ◽  
pp. 34-42 ◽  
Author(s):  
Edward Laane ◽  
Edneia Tani ◽  
Elisabet Björklund ◽  
Göran Elmberger ◽  
Hele Everaus ◽  
...  
Keyword(s):  
Hodgkin’S Lymphoma ◽  
Hodgkin's Lymphoma ◽  
Fine Needle ◽  
Flow Cytometric Immunophenotyping ◽  
Fine Needle Aspirates ◽  
Flow Cytometric ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

Estimation of Growth Fraction in Fine Needle Aspirates from Non-Hodgkin’s Lymphoma Using Anti–Proliferating Cell Nuclear Antigen

1996 ◽  
Vol 40 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Fernando Carlos Schmitt ◽  
Silvia Helena Rabenhorst ◽  
Sueli Aparecida Maeda ◽  
Vergilio Colturato ◽  
Ligia Niero Melo
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Hodgkin’S Lymphoma ◽  
Hodgkin's Lymphoma ◽  
Nuclear Antigen ◽  
Growth Fraction ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma ◽  
Proliferating Cell

Clinical Significance of Monoclonality by Flow Cytometry in Histologically Negative Bone Marrow in Patients with Diffuse B Cell Non Hodgkin’s Lymphoma.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4686-4686
Author(s):  
Meir Lahav ◽  
Michael Lucianski ◽  
Judith Radnay ◽  
Hava Shapiro ◽  
Naomi Gronich ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
B Cell ◽  
Clinical Significance ◽  
Hodgkin’S Lymphoma ◽  
Clinical Course ◽  
Hodgkin's Lymphoma ◽  
Flow Cytometric ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

Abstract Bone marrow (BM) involvement in diffuse large B cell non Hodgkin’s lymphoma (DLBCL) results in stage IV staging and has negative impact on survival. Up to 40% of patients with DLBCL have BM involvement by routine histology of marrow biopsy. The identification of lymphoid monoclonality is widely used in diagnosis of lymphoma, by flow cytometric and molecular methods. The clinical significance of monoclonality by these methods has not been systematically addressed in clinical setting. An International Workshop of response criteria in non Hodgkin’s lymphoma recommends that histologically normal marrow with small clonal population detected by flow cytometry should be considered normal until there are clinical studies that demonstrate a different outcome for this group. Recently it was reported that clonality detected by PCR amplification carries worse prognosis in patients with DLBCL. In our institution bone marrow biopsies of all patients with lymphoma are routinely evaluated by flow cytometric analysis since 1993. We evaluated the clinical course of all our patients with DLBCL diagnosed or followed up between 1993 and 2004 and compared the clinical course of patients with histologically positive marrow, histologically and cytometry negative marrow and histologically negative marrow and monoclonal lymphoid population detected by flow cytometry. Monoclonality was defined as ration of light chain expression kappa: lambda>3:1 or lambda: kappa > 2:1 in at least 2% of the gated population. Selected cells were analyzed by two or three color combinations: CD5 versus CD19, CD20 versus CD10 and kappa chain versus lambda chain occasionally with the addition of CD19 or CD20. Among 110 patients, 80 had negative marrow histologically and on cytometry (BM-FC-), 16 were positive on both parameters (BM+FC+) and 14 were histologically negative and cytometry positive (BM-FC+). All three groups were similar in parameters as age, performance status, hemoglobin and LDH levels. BM-FC- and BM-FC+ were similar in IPI scores. BM-FC- patients had significantly longer survival time that BM+FC+ and BM-FC+ patients. There was no significant difference in survival of BM+FC+ and BM-FC+ patients. We conclude that the clinical outcome of patients with DLBCL with histologically normal bone marrow and monoclonality on flow cytometry is similar to those with histologically positive marrow. These results may imply that lymphoid monoclonality on flow cytometry should be regarded as bone marrow involvement and treated as such.

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