scholarly journals Contribution of 3H-thymidine labelling index and flow cytometric S-phase in predicting survival of patients with non-Hodgkin's lymphoma

1992 ◽  
Vol 66 (4) ◽  
pp. 680-684 ◽  
Author(s):  
A Costa ◽  
R Silvestrini ◽  
R Giardini ◽  
G Messina-Gabrielli ◽  
P Boracchi ◽  
...  
Keyword(s):  
Hodgkin’S Lymphoma ◽  
S Phase ◽  
Labelling Index ◽  
Hodgkin's Lymphoma ◽  
Flow Cytometric ◽  
Thymidine Labelling Index ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma ◽  
Thymidine Labelling

scholarly journals Prognostic value of lymphocyte homing receptor and S phase fraction in non-Hodgkin's lymphoma

Blood ◽  
1990 ◽  
Vol 75 (7) ◽  
pp. 1549-1556
Author(s):  
S Jalkanen ◽  
H Joensuu ◽  
P Klemi
Keyword(s):  
Dna Content ◽  
Hodgkin’S Lymphoma ◽  
Nuclear Dna Content ◽  
S Phase ◽  
Hodgkin's Lymphoma ◽  
Phase Fraction ◽  
Lymphocyte Homing ◽  
High Endothelial Venules ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

Lymphocyte homing receptors (HRs) mediate lymphocyte binding to high endothelial venules, and control their circulation between the blood and the lymphoid organs. The role of HRs and nuclear DNA content in the spread and prognosis of non-Hodgkin's lymphoma was studied from paraffin-embedded tumor sections of 104 patients followed-up for the minimum of 5 years after the diagnosis. HR expression was analyzed by staining with a monoclonal antibody, Hermes-3, and DNA content by flow cytometry. Ten (10%) lymphomas were HR negative (HR-), 14 (13%) weakly (HR+/-), and 80 (77%) strongly positive (HR+). HR- lymphomas disseminated less often than HR+/- or HR+ lymphomas (P = .03), and their prognosis was more favorable (P = .03), although they often had a large S phase fraction (SPF), indicating a rapid proliferation rate. A large SPF (greater than 12%) was strongly associated with an unfavorable histologic type in Working Formulation (P = .0001) and poor survival (P = .006), whereas DNA aneuploidy was not. The 5-year survival rate corrected for intercurrent deaths was 61% in lymphomas with SPF less than 12% or with HR-, but only 15% if SPF was greater than 12% and HR+ (P less than .0001). In multivariate analysis stage (P less than .001), SPF (P = .002) and HR (P = .003) were the only independent prognostic factors.

scholarly journals Prognostic value of lymphocyte homing receptor and S phase fraction in non-Hodgkin's lymphoma

Blood ◽  
1990 ◽  
Vol 75 (7) ◽  
pp. 1549-1556 ◽  
Author(s):  
S Jalkanen ◽  
H Joensuu ◽  
P Klemi
Keyword(s):  
Dna Content ◽  
Hodgkin’S Lymphoma ◽  
Nuclear Dna Content ◽  
S Phase ◽  
Hodgkin's Lymphoma ◽  
Phase Fraction ◽  
Lymphocyte Homing ◽  
High Endothelial Venules ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

Abstract Lymphocyte homing receptors (HRs) mediate lymphocyte binding to high endothelial venules, and control their circulation between the blood and the lymphoid organs. The role of HRs and nuclear DNA content in the spread and prognosis of non-Hodgkin's lymphoma was studied from paraffin-embedded tumor sections of 104 patients followed-up for the minimum of 5 years after the diagnosis. HR expression was analyzed by staining with a monoclonal antibody, Hermes-3, and DNA content by flow cytometry. Ten (10%) lymphomas were HR negative (HR-), 14 (13%) weakly (HR+/-), and 80 (77%) strongly positive (HR+). HR- lymphomas disseminated less often than HR+/- or HR+ lymphomas (P = .03), and their prognosis was more favorable (P = .03), although they often had a large S phase fraction (SPF), indicating a rapid proliferation rate. A large SPF (greater than 12%) was strongly associated with an unfavorable histologic type in Working Formulation (P = .0001) and poor survival (P = .006), whereas DNA aneuploidy was not. The 5-year survival rate corrected for intercurrent deaths was 61% in lymphomas with SPF less than 12% or with HR-, but only 15% if SPF was greater than 12% and HR+ (P less than .0001). In multivariate analysis stage (P less than .001), SPF (P = .002) and HR (P = .003) were the only independent prognostic factors.

Clinical Significance of Monoclonality by Flow Cytometry in Histologically Negative Bone Marrow in Patients with Diffuse B Cell Non Hodgkin’s Lymphoma.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4686-4686
Author(s):  
Meir Lahav ◽  
Michael Lucianski ◽  
Judith Radnay ◽  
Hava Shapiro ◽  
Naomi Gronich ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
B Cell ◽  
Clinical Significance ◽  
Hodgkin’S Lymphoma ◽  
Clinical Course ◽  
Hodgkin's Lymphoma ◽  
Flow Cytometric ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

Abstract Bone marrow (BM) involvement in diffuse large B cell non Hodgkin’s lymphoma (DLBCL) results in stage IV staging and has negative impact on survival. Up to 40% of patients with DLBCL have BM involvement by routine histology of marrow biopsy. The identification of lymphoid monoclonality is widely used in diagnosis of lymphoma, by flow cytometric and molecular methods. The clinical significance of monoclonality by these methods has not been systematically addressed in clinical setting. An International Workshop of response criteria in non Hodgkin’s lymphoma recommends that histologically normal marrow with small clonal population detected by flow cytometry should be considered normal until there are clinical studies that demonstrate a different outcome for this group. Recently it was reported that clonality detected by PCR amplification carries worse prognosis in patients with DLBCL. In our institution bone marrow biopsies of all patients with lymphoma are routinely evaluated by flow cytometric analysis since 1993. We evaluated the clinical course of all our patients with DLBCL diagnosed or followed up between 1993 and 2004 and compared the clinical course of patients with histologically positive marrow, histologically and cytometry negative marrow and histologically negative marrow and monoclonal lymphoid population detected by flow cytometry. Monoclonality was defined as ration of light chain expression kappa: lambda>3:1 or lambda: kappa > 2:1 in at least 2% of the gated population. Selected cells were analyzed by two or three color combinations: CD5 versus CD19, CD20 versus CD10 and kappa chain versus lambda chain occasionally with the addition of CD19 or CD20. Among 110 patients, 80 had negative marrow histologically and on cytometry (BM-FC-), 16 were positive on both parameters (BM+FC+) and 14 were histologically negative and cytometry positive (BM-FC+). All three groups were similar in parameters as age, performance status, hemoglobin and LDH levels. BM-FC- and BM-FC+ were similar in IPI scores. BM-FC- patients had significantly longer survival time that BM+FC+ and BM-FC+ patients. There was no significant difference in survival of BM+FC+ and BM-FC+ patients. We conclude that the clinical outcome of patients with DLBCL with histologically normal bone marrow and monoclonality on flow cytometry is similar to those with histologically positive marrow. These results may imply that lymphoid monoclonality on flow cytometry should be regarded as bone marrow involvement and treated as such.

S-phase fraction by the labeling index as a predictive factor for progression and survival in low grade non-Hodgkin's lymphoma

Cancer ◽  
1995 ◽  
Vol 76 (6) ◽  
pp. 1059-1064 ◽  
Author(s):  
Thomas E. Witzig ◽  
Thomas M. Habermann ◽  
Paul J. Kurtin ◽  
Georgene Schroeder ◽  
Mary J. Stenson ◽  
...  
Keyword(s):  
Predictive Factor ◽  
Hodgkin’S Lymphoma ◽  
S Phase ◽  
Labeling Index ◽  
Hodgkin's Lymphoma ◽  
Phase Fraction ◽  
Low Grade ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

scholarly journals Heterogeneity of non-Hodgkin's lymphoma probed by nucleic acid cytometry

Blood ◽  
1985 ◽  
Vol 65 (5) ◽  
pp. 1090-1096 ◽  
Author(s):  
J Srigley ◽  
B Barlogie ◽  
JJ Butler ◽  
B Osborne ◽  
M Blick ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Proliferative Activity ◽  
Hodgkin’S Lymphoma ◽  
Hodgkin's Lymphoma ◽  
Double Stranded Rna ◽  
Flow Cytometric ◽  
Rna Content ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma ◽  
Cellular Dna

Flow cytometric analyses of cellular DNA, RNA, and double-stranded RNA content were performed on lymph nodes and extranodal tissue from 177 patients with non-Hodgkin's lymphoma. With increasing histologic grade, a higher incidence of aneuploidy, higher proliferative activity, and higher total and double-stranded RNA content were found. Despite considerable cytometric heterogeneity within histologic grades and morphologic subdivisions, conformity between cytometric and morphologic classifications was observed in 85% of cases. Among intermediate-grade and high-grade lymphomas, increased proliferative activity and diploidy were associated with more frequent responses to treatment. Thus, nucleic acid-derived parameters relate to morphologic subtypes and permit an objective approach to lymphoma classification based on ploidy, proliferation, and RNA characteristics that also had prognostic implications.

Effect of treatment on the prognostic value of S-phase fraction in non-Hodgkin's lymphoma.

1994 ◽  
Vol 12 (10) ◽  
pp. 2167-2175 ◽  
Author(s):  
H Joensuu ◽  
R Ristamäki ◽  
K O Söderström ◽  
S Jalkanen
Keyword(s):  
Cell Proliferation ◽  
Survival Rate ◽  
Prognostic Value ◽  
Hodgkin’S Lymphoma ◽  
S Phase ◽  
Hodgkin's Lymphoma ◽  
High Grade ◽  
Histologic Grading ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

PURPOSE To investigate the prognostic value of cell proliferation rate in non-Hodgkin's lymphoma, study its association with histologic classification, and investigate whether its predictive value is influenced by the type of treatment given. PATIENTS AND METHODS The S-phase fraction (SPF) size was determined by DNA flow cytometry from paraffin-embedded tissue obtained at diagnosis from 490 patients with non-Hodgkin's lymphoma, diagnosed in a defined geographic area from 1970 to 1991. Clinical data were collected from hospital records and the files of the Finnish Cancer Registry. RESULTS SPF size correlated well with histologic grading performed either according to the Working Formulation or Kiel classification (P < .0001 for both). The mean SPFs of low-, intermediate-, and high-grade malignant lymphomas were 4.9% (95% confidence interval [CI], 4.2% to 5.5%), 10.3% (95% CI, 9.3% to 11.4%), and 15.5% (95% CI, 14.0% to 16.9%), respectively. Lymphomas with an SPF lower than the median (7.9%) had a 58% 5-year and 44% 15-year survival rate, whereas those with an SPF larger than the median had a 44% 5-year and 40% 15-year survival rate (P < .0001). SPF size was not significantly associated with prognosis in some subgroups, such as among patients treated primarily with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) (n = 114) or cyclophosphamide, vincristine, and prednisone (COP) (n = 124) with or without radiotherapy (P > .05), whereas a stronger association was found among patients with stage I or II lymphoma treated with radiotherapy only (n = 100; P = .003) and among patients with stage III or IV lymphoma who did not receive chemotherapy (n = 44; P < .0001). In multivariate analyses that included the factors used to construct the International Prognostic Index, SPF had independent prognostic value both in low-grade and intermediate- or high-grade lymphomas, but not in the subset of patients treated with combination chemotherapy with or without radiotherapy. CONCLUSION Cell proliferation rate measured as SPF is closely associated with histologic grading in non-Hodgkin's lymphoma, and it has independent prognostic value. The treatment given influences considerably the prognostic value of SPF.

scholarly journals Heterogeneity of non-Hodgkin's lymphoma probed by nucleic acid cytometry

Blood ◽  
1985 ◽  
Vol 65 (5) ◽  
pp. 1090-1096 ◽  
Author(s):  
J Srigley ◽  
B Barlogie ◽  
JJ Butler ◽  
B Osborne ◽  
M Blick ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Proliferative Activity ◽  
Hodgkin’S Lymphoma ◽  
Hodgkin's Lymphoma ◽  
Double Stranded Rna ◽  
Flow Cytometric ◽  
Rna Content ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma ◽  
Cellular Dna

Abstract Flow cytometric analyses of cellular DNA, RNA, and double-stranded RNA content were performed on lymph nodes and extranodal tissue from 177 patients with non-Hodgkin's lymphoma. With increasing histologic grade, a higher incidence of aneuploidy, higher proliferative activity, and higher total and double-stranded RNA content were found. Despite considerable cytometric heterogeneity within histologic grades and morphologic subdivisions, conformity between cytometric and morphologic classifications was observed in 85% of cases. Among intermediate-grade and high-grade lymphomas, increased proliferative activity and diploidy were associated with more frequent responses to treatment. Thus, nucleic acid-derived parameters relate to morphologic subtypes and permit an objective approach to lymphoma classification based on ploidy, proliferation, and RNA characteristics that also had prognostic implications.

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