scholarly journals Validation of a Novel, Flash‐Freezing Method: Aluminum Platform

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Ahmet Imrali ◽  
Christine S. Hughes ◽  
Abigail S. Coetzee ◽  
Francesca R. Delvecchio ◽  
Amina Saad ◽  
...  
Keyword(s):  
Freezing Method

Methods for making stereoslides and -prints, with freeze-etched olfactory epithelium as example

1984 ◽  
Vol 42 ◽  
pp. 704-705
Author(s):  
Bert Ph. M. Menco ◽  
Ido F. Menco ◽  
Frans L.T. Verdonk
Keyword(s):  
Electron Microscope ◽  
Olfactory Epithelium ◽  
Three Dimensional ◽  
Supporting Cell ◽  
Structural Features ◽  
Extensive Study ◽  
Sprague Dawley ◽  
Freezing Method ◽  
Respiratory Epithelia ◽  
Three Dimensional Presentation

Previously we presented an extensive study of the distributions of intramembranous particles of structures in apical surfaces of nasal olfactory and respiratory epithelia of the Sprague-Dawley rat. For the same structures these distributions were compared in samples which were i) chemically fixed and cryo-protected with glycerol before cryo-fixation, after excision, and ii)ultra-rapidly frozen by means of the slam-freezing method. Since a three-dimensional presentation markedly improves visualization of structural features micrographs were presented as stereopairs. Two exposures were made by tiling the sample stage of the electron microscope 6° in either direction with an eucentric goniometer. The negatives (Agfa Pan 25 Professional) were reversed with Kodak Technical Pan Film 2415 developed in D76 1:1. The prints were made from these reversed negatives. As an example tight-junctional features of an olfactory supporting cell in a region where this cell conjoined with two other cells are presented (Fig. 1).

scholarly journals An easy and reliable whole blood freezing method for flow cytometry immuno‐phenotyping and functional analyses

2021 ◽  
Author(s):  
Cecile Braudeau ◽  
Nina Salabert‐Le Guen ◽  
Justine Chevreuil ◽  
Marie Rimbert ◽  
Jerome C. Martin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Freezing Method ◽  
Functional Analyses

Treatment of biofuel production wastewater by a combined freezing method for resources recovery and waste reduction

2021 ◽  
Vol 774 ◽  
pp. 145173
Author(s):  
Wei Yuan ◽  
Lehua Zhang ◽  
Yulong Chang ◽  
Hongpeng Ma ◽  
Pengbo Fu ◽  
...  
Keyword(s):  
Waste Reduction ◽  
Biofuel Production ◽  
Freezing Method

Analysis of Water Transport in the Vicinity of Micro-Porous Layer in PEFC With Freezing Method

2013 ◽  
Author(s):  
Yusuke Aoyama ◽  
Kengo Suzuki ◽  
Yutaka Tabe ◽  
Takemi Chikahisa
Keyword(s):  
Catalyst Layer ◽  
Cathode Side ◽  
Cross Sectional ◽  
Porous Layers ◽  
Freezing Method ◽  
Water Condensation ◽  
Flow Channels ◽  
Condensed Water ◽  
Electronic Microscope

This paper examines the role of micro porous layers (MPLs) in Polymer Electrode Fuel Cells (PEFCs) by observing the cross-sectional distribution of condensed water inside a cathode side MPL In addition, the forms of water condensation in the vicinity of a MPL are also compared between two places, under flow channels and under lands, by observing both inside the MPL and an interface between the MPL and a catalyst layer (CL). The freezing method and a cryo-scanning electronic microscope (cryo-SEM) are used for the observation. The result under the non-flooded condition shows that condensed water does not accumulate inside the MPL. This result indicates that the water produced by PEFC power generation passes through the MPL as vapor state under non-flooded conditions.

Ultrastructure of Bovine Embryos Frozen and Thawed by a Two-Step Freezing Method

1986 ◽  
Vol 125 (1) ◽  
pp. 27-31 ◽  
Author(s):  
P. Hyttel ◽  
H. Lehn-Jensen ◽  
T. Greve
Keyword(s):  
Bovine Embryos ◽  
Freezing Method

High-performance ionic liquid-based nanocomposite polymer electrolytes with anisotropic ionic conductivity prepared by coupling liquid crystal self-templating with unidirectional freezing

2015 ◽  
Vol 3 (5) ◽  
pp. 2128-2134 ◽  
Author(s):  
Hongzan Song ◽  
Ningning Zhao ◽  
Weichao Qin ◽  
Bing Duan ◽  
Xiaoya Ding ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Liquid Crystal ◽  
Ionic Conductivity ◽  
Polymer Electrolytes ◽  
High Performance ◽  
Vacuum Degassing ◽  
Freezing Method ◽  
Nanocomposite Polymer ◽  
Unidirectional Freezing ◽  
Nanocomposite Polymer Electrolytes

High-performance NCPE has been fabricated by using unidirectional freezing method, liquid crystal self-templating approach and vacuum degassing method.

Directional freezing of spermatozoa and embryos

2014 ◽  
Vol 26 (1) ◽  
pp. 83 ◽  
Author(s):  
Amir Arav ◽  
Joseph Saragusty
Keyword(s):  
Cooling Rate ◽  
Drop Size ◽  
Mechanical Damage ◽  
Rapid Freezing ◽  
Wild Animals ◽  
Microscope Slide ◽  
Freezing Method ◽  
Wide Range ◽  
Sexed Semen ◽  
Directional Freezing

Directional freezing is based on a simple thermodynamic principle whereby the sample is moved through a predetermined temperature gradient at a velocity that determines the cooling rate. Directional freezing permits a precise and uniform cooling rate in small- and large-volume samples. It avoids supercooling and reduces mechanical damage caused by crystallisation. Directional solidification was used to date for slow and rapid freezing, as well as for vitrification of oocytes and embryos by means of the minimum drop size technique: small drops are placed on a microscope slide that is moved at high velocity from the hot base to the cold base. Sperm samples from a wide range of domestic and wild animals were successfully cryopreserved using the directional freezing method. The bovine sexed semen industry may benefit from the increased survival of spermatozoa after directional freezing.

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