Trajectory of spinocerebellar fibers passing through the inferior and superior cerebellar peduncles in the rat spinal cord: A study using horseradish peroxidase with pedunculotomy

1991 ◽  
Vol 304 (1) ◽  
pp. 147-160 ◽  
Author(s):  
Jinzo Yamada ◽  
Kuniaki Shirao ◽  
Taiko Kitamura ◽  
Hitoshi Sato
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Rat Spinal Cord ◽  
Cerebellar Peduncles ◽  
Spinocerebellar Fibers

scholarly journals Direct immunocytochemistry with a horseradish peroxidase-conjugated monoclonal antibody against substance P.

1982 ◽  
Vol 30 (12) ◽  
pp. 1211-1216 ◽  
Author(s):  
D M Boorsma ◽  
A C Cuello ◽  
F W van Leeuwen
Keyword(s):  
Spinal Cord ◽  
Monoclonal Antibody ◽  
Substance P ◽  
Horseradish Peroxidase ◽  
Nerve Fibers ◽  
Model System ◽  
Gel Chromatography ◽  
Rat Spinal Cord ◽  
Fixed Tissue ◽  
Triton X

The procedure for the isolation and conjugation of the anti-substance P monoclonal antibody NC1/34 with the enzyme horseradish peroxidase (HRP) is described. This resulted in a molecular complex of monoclonal antibody/HRP of 1:1. This conjugate was of approximately 400,000 daltons, as estimated by gel chromatography. Practically all the isolated antibody was coupled to HRP. The conjugate was tested both in a model system where CNBr-activated Sepharose beads were coupled to substance P and on fixed tissue preparations from the rat spinal cord and medulla oblongata. The conjugate revealed staining in nerve fibers in areas known to contain substance P. The best immunohistochemical results were obtained by prolonged incubations at 12 degrees C in the presence of 0.1% Triton X-100. The preabsorption of the conjugate with substance P obliterated the reaction.

Electrophysiological and horseradish peroxidase-tracing studies of nerve regeneration through alginate-filled gap in adult rat spinal cord

2002 ◽  
Vol 318 (3) ◽  
pp. 121-124 ◽  
Author(s):  
Yoshihisa Suzuki ◽  
Miyako Kitaura ◽  
Sufan Wu ◽  
Kazuya Kataoka ◽  
Kyoko Suzuki ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Nerve Regeneration ◽  
Rat Spinal Cord ◽  
Adult Rat ◽  
Adult Rat Spinal Cord

Distribution of intraventricularly injected horseradish peroxidase in cerebrospinal fluid compartments of the rat spinal cord

1992 ◽  
Vol 270 (3) ◽  
pp. 485-494 ◽  
Author(s):  
M. Cifuentes ◽  
P. Fern�ndez-LLebrez ◽  
J. P�rez ◽  
J. M. P�rez-F�gares ◽  
E. M. Rodr�guez
Keyword(s):  
Spinal Cord ◽  
Cerebrospinal Fluid ◽  
Horseradish Peroxidase ◽  
Rat Spinal Cord ◽  
Fluid Compartments

scholarly journals Ultrastructural visualization of anterogradely transported horseradish peroxidase in developing corticospinal tract of rat.

1987 ◽  
Vol 35 (5) ◽  
pp. 623-626 ◽  
Author(s):  
E A Joosten ◽  
A A Gribnau ◽  
P J Dederen
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Phosphate Buffer ◽  
Corticospinal Tract ◽  
Neural Tissue ◽  
Ultrastructural Level ◽  
Rat Spinal Cord ◽  
Electron Microscopic ◽  
Stabilizing Agent ◽  
Satisfactory Method

Until now a satisfactory method for electron microscopic (EM) detection of anterogradely transported horseradish peroxidase (HRP) in developing neural tissue, using sensitive chromogen tetramethylbenzidine (TMB), has not been described. Use of the stabilizing agent ammoniumheptamolybdate (AHM), in combination with a modified prolonged osmication [4 hr at pH 5.0 in 0.1 M phosphate buffer (PB)] made possible visualization of HRP-TMB-(AHM) reaction product at the ultrastructural level in outgrowing corticospinal tract (CST) fibers of young postnatal rat. This reaction product appeared to be very distinctive and clearly detectable, making ultrastructural identification of HRP-labeled outgrowing CST fibers in rat spinal cord rather easy. In addition, the procedure described in this report preserves the ultrastructural details of the developing neural tissue.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

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