scholarly journals Ultrastructural visualization of anterogradely transported horseradish peroxidase in developing corticospinal tract of rat.

1987 ◽  
Vol 35 (5) ◽  
pp. 623-626 ◽  
Author(s):  
E A Joosten ◽  
A A Gribnau ◽  
P J Dederen
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Phosphate Buffer ◽  
Corticospinal Tract ◽  
Neural Tissue ◽  
Ultrastructural Level ◽  
Rat Spinal Cord ◽  
Electron Microscopic ◽  
Stabilizing Agent ◽  
Satisfactory Method

Until now a satisfactory method for electron microscopic (EM) detection of anterogradely transported horseradish peroxidase (HRP) in developing neural tissue, using sensitive chromogen tetramethylbenzidine (TMB), has not been described. Use of the stabilizing agent ammoniumheptamolybdate (AHM), in combination with a modified prolonged osmication [4 hr at pH 5.0 in 0.1 M phosphate buffer (PB)] made possible visualization of HRP-TMB-(AHM) reaction product at the ultrastructural level in outgrowing corticospinal tract (CST) fibers of young postnatal rat. This reaction product appeared to be very distinctive and clearly detectable, making ultrastructural identification of HRP-labeled outgrowing CST fibers in rat spinal cord rather easy. In addition, the procedure described in this report preserves the ultrastructural details of the developing neural tissue.

Reformation of specific neuromuscular connections during axolotl limb regeneration: evidence that the first contacts are correct

Development ◽  
1988 ◽  
Vol 103 (2) ◽  
pp. 365-377
Author(s):  
S. Wilson ◽  
M. Jesani ◽  
N. Holder
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Limb Regeneration ◽  
Microscopic Analysis ◽  
Motor Neurone ◽  
Extensor Digitorum ◽  
Limb Muscle ◽  
Proximal Muscle ◽  
Electron Microscopic ◽  
Neuronal Tracing

Retrograde neuronal tracing with horseradish peroxidase was used to determine the position in the spinal cord of the motor neurone pools of a proximal (biceps) and a distal (extensor digitorum) limb muscle at various times during axolotl limb regeneration. It was found that from the earliest stages of muscle redifferentiation (as judged by light and electron microscopic analysis) the vast majority of axons innervating the regenerating muscles came from cells within the bounds of the normal motor neurone pool for each muscle. A few incorrect projections were noted in that the regenerating proximal muscle was sometimes innervated by some cells caudal to its normal motor neurone pool. The results are discussed in terms of mechanisms that may be operating in the regenerating limb to ensure that specific neuromuscular connections are made.

Endomorphin-2 immunoreactivity in the cervical dorsal horn of the rat spinal cord at the electron microscopic level

Neuroscience ◽  
2002 ◽  
Vol 113 (3) ◽  
pp. 593-605 ◽  
Author(s):  
Q.P Wang ◽  
J.E Zadina ◽  
J.-L Guan ◽  
A.J Kastin ◽  
H Funahashi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Rat Spinal Cord ◽  
Electron Microscopic

scholarly journals Direct immunocytochemistry with a horseradish peroxidase-conjugated monoclonal antibody against substance P.

1982 ◽  
Vol 30 (12) ◽  
pp. 1211-1216 ◽  
Author(s):  
D M Boorsma ◽  
A C Cuello ◽  
F W van Leeuwen
Keyword(s):  
Spinal Cord ◽  
Monoclonal Antibody ◽  
Substance P ◽  
Horseradish Peroxidase ◽  
Nerve Fibers ◽  
Model System ◽  
Gel Chromatography ◽  
Rat Spinal Cord ◽  
Fixed Tissue ◽  
Triton X

The procedure for the isolation and conjugation of the anti-substance P monoclonal antibody NC1/34 with the enzyme horseradish peroxidase (HRP) is described. This resulted in a molecular complex of monoclonal antibody/HRP of 1:1. This conjugate was of approximately 400,000 daltons, as estimated by gel chromatography. Practically all the isolated antibody was coupled to HRP. The conjugate was tested both in a model system where CNBr-activated Sepharose beads were coupled to substance P and on fixed tissue preparations from the rat spinal cord and medulla oblongata. The conjugate revealed staining in nerve fibers in areas known to contain substance P. The best immunohistochemical results were obtained by prolonged incubations at 12 degrees C in the presence of 0.1% Triton X-100. The preabsorption of the conjugate with substance P obliterated the reaction.

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