Distribution of intraventricularly injected horseradish peroxidase in cerebrospinal fluid compartments of the rat spinal cord

1992 ◽  
Vol 270 (3) ◽  
pp. 485-494 ◽  
Author(s):  
M. Cifuentes ◽  
P. Fern�ndez-LLebrez ◽  
J. P�rez ◽  
J. M. P�rez-F�gares ◽  
E. M. Rodr�guez
Keyword(s):  
Spinal Cord ◽  
Cerebrospinal Fluid ◽  
Horseradish Peroxidase ◽  
Rat Spinal Cord ◽  
Fluid Compartments

scholarly journals Direct immunocytochemistry with a horseradish peroxidase-conjugated monoclonal antibody against substance P.

1982 ◽  
Vol 30 (12) ◽  
pp. 1211-1216 ◽  
Author(s):  
D M Boorsma ◽  
A C Cuello ◽  
F W van Leeuwen
Keyword(s):  
Spinal Cord ◽  
Monoclonal Antibody ◽  
Substance P ◽  
Horseradish Peroxidase ◽  
Nerve Fibers ◽  
Model System ◽  
Gel Chromatography ◽  
Rat Spinal Cord ◽  
Fixed Tissue ◽  
Triton X

The procedure for the isolation and conjugation of the anti-substance P monoclonal antibody NC1/34 with the enzyme horseradish peroxidase (HRP) is described. This resulted in a molecular complex of monoclonal antibody/HRP of 1:1. This conjugate was of approximately 400,000 daltons, as estimated by gel chromatography. Practically all the isolated antibody was coupled to HRP. The conjugate was tested both in a model system where CNBr-activated Sepharose beads were coupled to substance P and on fixed tissue preparations from the rat spinal cord and medulla oblongata. The conjugate revealed staining in nerve fibers in areas known to contain substance P. The best immunohistochemical results were obtained by prolonged incubations at 12 degrees C in the presence of 0.1% Triton X-100. The preabsorption of the conjugate with substance P obliterated the reaction.

Electrophysiological and horseradish peroxidase-tracing studies of nerve regeneration through alginate-filled gap in adult rat spinal cord

2002 ◽  
Vol 318 (3) ◽  
pp. 121-124 ◽  
Author(s):  
Yoshihisa Suzuki ◽  
Miyako Kitaura ◽  
Sufan Wu ◽  
Kazuya Kataoka ◽  
Kyoko Suzuki ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Nerve Regeneration ◽  
Rat Spinal Cord ◽  
Adult Rat ◽  
Adult Rat Spinal Cord

scholarly journals Nanoparticle Estrogen in Rat Spinal Cord Injury Elicits Rapid Anti-Inflammatory Effects in Plasma, Cerebrospinal Fluid, and Tissue

2015 ◽  
Vol 32 (18) ◽  
pp. 1413-1421 ◽  
Author(s):  
April Cox ◽  
Abhay Varma ◽  
John Barry ◽  
Alexey Vertegel ◽  
Naren Banik
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cerebrospinal Fluid ◽  
Rat Spinal Cord ◽  
Cord Injury ◽  
Anti Inflammatory
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