Distribution and ultrastructure of neurons in opossum piriform cortex displaying immunoreactivity to GABA and GAD and high-affinity tritiated GABA uptake

1987 ◽  
Vol 266 (2) ◽  
pp. 269-290 ◽  
Author(s):  
Lewis B. Haberly ◽  
David J. Hansen ◽  
Sherry L. Feig ◽  
Susan Presto
Keyword(s):  
Piriform Cortex ◽  
Gaba Uptake ◽  
High Affinity

Modulation of high affinity GABA uptake into hippocampal synaptosomes by glucocorticoids

1978 ◽  
Vol 3 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Ann L. Miller ◽  
Claude Chaptal ◽  
Bruce S. McEwen ◽  
Ernest J. Peck
Keyword(s):  
Gaba Uptake ◽  
High Affinity

ZAPA, a substrate for the neuronal high affinity GABA uptake system in rat brain slices

1991 ◽  
Vol 18 (1) ◽  
pp. 63-67 ◽  
Author(s):  
R ALLAN ◽  
H DICKENSON ◽  
R DUKE ◽  
G JOHNSTON
Keyword(s):  
Rat Brain ◽  
Brain Slices ◽  
Gaba Uptake ◽  
Uptake System ◽  
High Affinity

scholarly journals Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C

1991 ◽  
Vol 275 (2) ◽  
pp. 435-439 ◽  
Author(s):  
J Gomeza ◽  
M Casado ◽  
C Gimenez ◽  
C Aragon
Keyword(s):  
Phospholipase C ◽  
Glial Cells ◽  
Kinase Inhibitor ◽  
Primary Cultures ◽  
Aminobutyric Acid ◽  
Gaba Uptake ◽  
Acid Uptake ◽  
Gamma Aminobutyric Acid ◽  
Gaba Transport ◽  
High Affinity

The effects of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), on high-affinity Na(+)-dependent gamma-aminobutyric acid (GABA) uptake were investigated in primary cultures of neurons and glial cells from rat brain cortex. Incubation of glial cells with PMA led to concentration- and time-dependent decreases in the GABA transport in glial cells. This effect could be completely suppressed by addition of the PKC inhibitor H7. The PMA effects could be mimicked by oleoylacetylglycerol, the diacylglycerol kinase inhibitor R59022 and exogenous phospholipase C. Treatment with PMA did not affect GABA transport in neuronal cells.

scholarly journals High-affinity GABA uptake in a subpopulation of somatostatin cells in rat pancreas.

1989 ◽  
Vol 37 (7) ◽  
pp. 1133-1139 ◽  
Author(s):  
P Gilon ◽  
C Remacle
Keyword(s):  
High Resolution ◽  
Gaba Uptake ◽  
Low Resolution ◽  
Paraffin Sections ◽  
Rat Pancreas ◽  
High Affinity ◽  
Langerhans Islets ◽  
Somatostatin Cells ◽  
High Resolution Autoradiography ◽  
Silver Grains

The aim of this study was to localize the high-affinity uptake of [3H]-GABA in Langerhans islets of rats aged 2.5, 7.5, and 75 days. On high-resolution autoradiography, cells presenting characteristic somatostatin granules were labeled, whereas others containing similar granules appeared nearly devoid of silver grains. Immunogold detection with antisomatostatin antibodies and high-resolution autoradiography suggested that uptake of GABA is indeed performed by somatostatin cells. To test the heterogeneity of uptake frequency in somatostatin cells, a second approach, coupling immunohistochemistry with anti-somatostatin, anti-PP, anti-glucagon, anti-glicentin, and anti-CCK antibodies, and low-resolution autoradiography, was applied on paraffin sections. It demonstrated that the uptake ability is not characteristic of all the somatostatin cells but of only a subpopulation of them. A few cells not immunoreactive to the anti-somatostatin antiserum also appeared to be able to take up GABA. Moreover, except for a rare few, the PP-glucagon-, glicentin-, and CCK-39-immunoreactive cells were not labeled by autoradiography.

Localization of high-affinity GABA uptake and GABA content in the rat duodenum during development

1987 ◽  
Vol 249 (3) ◽  
pp. 593-600 ◽  
Author(s):  
P. Gilon ◽  
B. Reusens-Billen ◽  
C. Remacle ◽  
Ph. Janssens de Varebeke ◽  
G. Pauwels ◽  
...  
Keyword(s):  
Gaba Uptake ◽  
High Affinity ◽  
Rat Duodenum ◽  
Gaba Content

Nipecotic acid and 2,4-diaminobutyric acid enhance the actions of muscimol on cerebral cortical neurons

1978 ◽  
Vol 56 (3) ◽  
pp. 443-446 ◽  
Author(s):  
G. G. Yarbrough
Keyword(s):  
New York ◽  
Nervous System ◽  
Amino Acid ◽  
Cortical Neurons ◽  
Gaba Uptake ◽  
Nipecotic Acid ◽  
Diaminobutyric Acid ◽  
Selective Blockade ◽  
High Affinity ◽  
Cerebral Cortical Neurons

Iontophoretically applied muscimol exerted a potent inhibitory action on the firing of spontaneously active cerebral cortical neurons of rats. On the basis of ejection currents employed, muscimol was considerably more potent than γ-aminobutyric acid (GABA), and the inhibitions produced by muscimol were frequently of a longer duration than those observed with GABA. Nipecotic acid and diaminobutyric acid (DABA) are potent inhibitors of high-affinity GABA uptake systems and muscimol is not thought to be a substrate for high-affinity GABA uptake (JOHNSTON, G. A. R. 1976. Physiolic pharmacology of GABA and its antagonists in the vertebrate nervous system. In GABA in nervous system function. Edited by E. Roberts, T. Chase, and D. B. Tower. Raven Press, New York). However, nipecotic acid and DABA consistently enhanced the inhibitory effects of both GABA and muscimol on cortical neurons while not affecting monoamine or adenosine 5′-monophosphate induced inhibitions. These findings suggest that iontophoretically applied nipecotic acid and DABA have some specificity for amino acid mechanisms in the cortex, but their actions appear to be more complex than can be explained by a selective blockade of GABA uptake processes. Thus, the uptake blockers may be exerting a weak and not readily detectable agonist action at amino acid receptors. Alternatively, the actions of iontophoretically applied muscimol may be partially terminated by uptake through GABA uptake systems.

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