scholarly journals CAPER-α alternative splicing regulates the expression of vascular endothelial growth factor165 in Ewing sarcoma cells

Cancer ◽  
2011 ◽  
Vol 118 (8) ◽  
pp. 2106-2116 ◽  
Author(s):  
Gangxiong Huang ◽  
Zhichao Zhou ◽  
Hua Wang ◽  
Eugenie S. Kleinerman
Keyword(s):  
Alternative Splicing ◽  
Ewing Sarcoma ◽  
Vascular Endothelial ◽  
Sarcoma Cells

scholarly journals Poison-Exon Inclusion in DHX9 Reduces Its Expression and Sensitizes Ewing Sarcoma Cells to Chemotherapeutic Treatment

Cells ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 328 ◽  
Author(s):  
Ramona Palombo ◽  
Veronica Verdile ◽  
Maria Paola Paronetto
Keyword(s):  
Alternative Splicing ◽  
Ewing Sarcoma ◽  
Splicing Factors ◽  
Mrna Variants ◽  
Aggressive Tumor ◽  
Chemotherapeutic Treatment ◽  
Sarcoma Cells ◽  
Combinatorial Mechanism ◽  
Worse Prognosis

Alternative splicing is a combinatorial mechanism by which exons are joined to produce multiple mRNA variants, thus expanding the coding potential and plasticity of eukaryotic genomes. Defects in alternative splicing regulation are associated with several human diseases, including cancer. Ewing sarcoma is an aggressive tumor of bone and soft tissue, mainly affecting adolescents and young adults. DHX9 is a key player in Ewing sarcoma malignancy, and its expression correlates with worse prognosis in patients. In this study, by screening a library of siRNAs, we have identified splicing factors that regulate the alternative inclusion of a poison exon in DHX9 mRNA, leading to its downregulation. In particular, we found that hnRNPM and SRSF3 bind in vivo to this poison exon and suppress its inclusion. Notably, DHX9 expression correlates with that of SRSF3 and hnRNPM in Ewing sarcoma patients. Furthermore, downregulation of SRSF3 or hnRNPM inhibited DHX9 expression and Ewing sarcoma cell proliferation, while sensitizing cells to chemotherapeutic treatment. Hence, our study suggests that inhibition of hnRNPM and SRSF3 expression or activity could be exploited as a therapeutic tool to enhance the efficacy of chemotherapy in Ewing sarcoma.

scholarly journals hnRNPM guides an alternative splicing program in response to inhibition of the PI3K/AKT/mTOR pathway in Ewing sarcoma cells

2017 ◽  
Vol 45 (21) ◽  
pp. 12270-12284 ◽  
Author(s):  
Ilaria Passacantilli ◽  
Paola Frisone ◽  
Elisa De Paola ◽  
Marco Fidaleo ◽  
Maria Paola Paronetto
Keyword(s):  
Alternative Splicing ◽  
Ewing Sarcoma ◽  
Mtor Pathway ◽  
Sarcoma Cells

Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells

2006 ◽  
Vol 155 (3) ◽  
pp. 493-504 ◽  
Author(s):  
Renata Taslerová ◽  
Stanislav Kozubek ◽  
Eva Bártová ◽  
Pavla Gajdušková ◽  
Roman Kodet ◽  
...  
Keyword(s):  
Ewing Sarcoma ◽  
Derivative Chromosome ◽  
Chromosome 11 ◽  
Genetic Elements ◽  
Sarcoma Cells

Single-cell RNA profiling identifies diverse cellular responses to EWSR1/FLI1 downregulation in Ewing sarcoma cells

2022 ◽  
Author(s):  
Roxane Khoogar ◽  
Fuyang Li ◽  
Yidong Chen ◽  
Myron Ignatius ◽  
Elizabeth R. Lawlor ◽  
...  
Keyword(s):  
Single Cell ◽  
Ewing Sarcoma ◽  
Cellular Responses ◽  
Rna Profiling ◽  
Sarcoma Cells

634 Regulation of alternative splicing by the Ewing Sarcoma Protein (EWS) and DNA damage

2010 ◽  
Vol 8 (5) ◽  
pp. 161
Author(s):  
J. Valcárcel ◽  
M.P. Paronetto ◽  
B. Miñana ◽  
E. Becchara ◽  
C. Ben-Dov
Keyword(s):  
Dna Damage ◽  
Alternative Splicing ◽  
Ewing Sarcoma

scholarly journals Immunostimulation by OX40 Ligand Transgenic Ewing Sarcoma Cells

2015 ◽  
Vol 5 ◽  
Author(s):  
Dajana Reuter ◽  
Martin S. Staege ◽  
Caspar D. Kühnöl ◽  
Jürgen Föll
Keyword(s):  
Ewing Sarcoma ◽  
Ox40 Ligand ◽  
Sarcoma Cells

scholarly journals Englerin A Inhibits EWS-FLI1 DNA Binding in Ewing Sarcoma Cells

2016 ◽  
Vol 291 (19) ◽  
pp. 10058-10066 ◽  
Author(s):  
Vittorio Caropreso ◽  
Emad Darvishi ◽  
Thomas J. Turbyville ◽  
Ranjala Ratnayake ◽  
Patrick J. Grohar ◽  
...  
Keyword(s):  
Dna Binding ◽  
Ewing Sarcoma ◽  
Englerin A ◽  
Sarcoma Cells

scholarly journals Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells

2018 ◽  
Author(s):  
Leonardo Horbach ◽  
Marialva Sinigaglia ◽  
Camila da Silva ◽  
Danielly Olguins ◽  
Lauro Gregianin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ewing Sarcoma ◽  
Chemotherapy Resistance ◽  
Sarcoma Cells
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