scholarly journals Gene expression changes associated with chemotherapy resistance in Ewing sarcoma cells

2018 ◽  
Author(s):  
Leonardo Horbach ◽  
Marialva Sinigaglia ◽  
Camila da Silva ◽  
Danielly Olguins ◽  
Lauro Gregianin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ewing Sarcoma ◽  
Chemotherapy Resistance ◽  
Sarcoma Cells

scholarly journals Gene expression and immunohistochemical analyses identify SOX2 as major risk factor for overall survival and relapse in Ewing sarcoma patients

EBioMedicine ◽  
2019 ◽  
Vol 47 ◽  
pp. 156-162 ◽  
Author(s):  
Giuseppina Sannino ◽  
Aruna Marchetto ◽  
Andreas Ranft ◽  
Susanne Jabar ◽  
Constanze Zacherl ◽  
...  
Keyword(s):  
Gene Expression ◽  
Overall Survival ◽  
Risk Factor ◽  
Ewing Sarcoma ◽  
Major Risk Factor ◽  
Immunohistochemical Analyses

Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells

2006 ◽  
Vol 155 (3) ◽  
pp. 493-504 ◽  
Author(s):  
Renata Taslerová ◽  
Stanislav Kozubek ◽  
Eva Bártová ◽  
Pavla Gajdušková ◽  
Roman Kodet ◽  
...  
Keyword(s):  
Ewing Sarcoma ◽  
Derivative Chromosome ◽  
Chromosome 11 ◽  
Genetic Elements ◽  
Sarcoma Cells

Single-cell RNA profiling identifies diverse cellular responses to EWSR1/FLI1 downregulation in Ewing sarcoma cells

2022 ◽  
Author(s):  
Roxane Khoogar ◽  
Fuyang Li ◽  
Yidong Chen ◽  
Myron Ignatius ◽  
Elizabeth R. Lawlor ◽  
...  
Keyword(s):  
Single Cell ◽  
Ewing Sarcoma ◽  
Cellular Responses ◽  
Rna Profiling ◽  
Sarcoma Cells

scholarly journals Biological Insights into Chemotherapy Resistance in Ovarian Cancer

2019 ◽  
Vol 20 (9) ◽  
pp. 2131 ◽  
Author(s):  
Michelle A. Glasgow ◽  
Peter Argenta ◽  
Juan E. Abrahante ◽  
Mihir Shetty ◽  
Shobhana Talukdar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Expression Profiles ◽  
Rna Extraction ◽  
Chemotherapy Resistance ◽  
Gene Expression Profiles ◽  
Gene Set Enrichment Analysis ◽  
Before And After ◽  
Tumor Gene Expression ◽  
Tumor Gene

The majority of patients with high-grade serous ovarian cancer (HGSOC) initially respond to chemotherapy; however, most will develop chemotherapy resistance. Gene signatures may change with the development of chemotherapy resistance in this population, which is important as it may lead to tailored therapies. The objective of this study was to compare tumor gene expression profiles in patients before and after treatment with neoadjuvant chemotherapy (NACT). Tumor samples were collected from six patients diagnosed with HGSOC before and after administration of NACT. RNA extraction and whole transcriptome sequencing was performed. Differential gene expression, hierarchical clustering, gene set enrichment analysis, and pathway analysis were examined in all of the samples. Tumor samples clustered based on exposure to chemotherapy as opposed to patient source. Pre-NACT samples were enriched for multiple pathways involving cell cycle growth. Post-NACT samples were enriched for drug transport and peroxisome pathways. Molecular subtypes based on the pre-NACT sample (differentiated, mesenchymal, proliferative and immunoreactive) changed in four patients after administration of NACT. Multiple changes in tumor gene expression profiles after exposure to NACT were identified from this pilot study and warrant further attention as they may indicate early changes in the development of chemotherapy resistance.

scholarly journals Anatomic Origin of Osteochondrogenic Progenitors Impacts Sensitivity to EWS-FLI1-Induced Transformation

Cancers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 313 ◽  
Author(s):  
Elise Pfaltzgraff ◽  
April Apfelbaum ◽  
Andrew Kassa ◽  
Jane Song ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Progenitor Cells ◽  
Malignant Transformation ◽  
Ewing Sarcoma ◽  
Hox Genes ◽  
High Expression ◽  
Wild Type ◽  
Superficial Zone ◽  
The Impact

Ewing sarcomas predominantly arise in pelvic and stylopod bones (i.e., femur and humerus), likely as a consequence of EWS-FLI1 oncogene-induced transformation of mesenchymal stem/progenitor cells (MSCs). MSCs located in the embryonic superficial zone cells (eSZ) of limbs express anatomically distinct posterior Hox genes. Significantly, high expression of posterior HOXD genes, especially HOXD13, is a hallmark of Ewing sarcoma. These data drove our hypothesis that Hox genes in posterior skeleton MSCs contribute to Ewing sarcoma tumorigenesis. We isolated eSZ cells from stylopod and zeugopod (i.e., tibia/fibula, radius/ulna) bones, from wild-type and Hoxd13 mutant embryos, and tested the impact of EWS-FLI1 transduction on cell proliferation, gene expression, and tumorigenicity. Our data demonstrate that both stylopod and zeugopod eSZ cells tolerate EWS-FLI1 but that stylopod eSZ cells are relatively more susceptible, demonstrating changes in proliferation and gene expression consistent with initiation of malignant transformation. Significantly, loss of Hoxd13 had no impact, showing that it is dispensable for the initiation of EWS-FLI1-induced transformation in mouse MSCs. These findings show that MSCs from anatomically distinct sites are differentially susceptible to EWS-FLI1-induced transformation, supporting the premise that the dominant presentation of Ewing sarcoma in pelvic and stylopod bones is attributable to anatomically-defined differences in MSCs.

scholarly journals Immunostimulation by OX40 Ligand Transgenic Ewing Sarcoma Cells

2015 ◽  
Vol 5 ◽  
Author(s):  
Dajana Reuter ◽  
Martin S. Staege ◽  
Caspar D. Kühnöl ◽  
Jürgen Föll
Keyword(s):  
Ewing Sarcoma ◽  
Ox40 Ligand ◽  
Sarcoma Cells
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