Enantioseparation of ofloxacin and its four related substances with ligand exchange-micellar electrokinetic chromatography using copper(II)-L-isoleucine complex as chiral selector

Chirality ◽  
2017 ◽  
Vol 29 (8) ◽  
pp. 422-429 ◽  
Author(s):  
Yongjing Liu ◽  
Xiaoying Wang
Keyword(s):  
Ligand Exchange ◽  
Micellar Electrokinetic Chromatography ◽  
Chiral Selector ◽  
Electrokinetic Chromatography ◽  
Related Substances

PolysodiumN-Undecanoyl-l-leucylvalinate:  A Versatile Chiral Selector for Micellar Electrokinetic Chromatography

2003 ◽  
Vol 75 (3) ◽  
pp. 379-387 ◽  
Author(s):  
Shahab A. Shamsi ◽  
Bertha C. Valle ◽  
Fereshteh Billiot ◽  
Isiah M. Warner
Keyword(s):  
Micellar Electrokinetic Chromatography ◽  
Chiral Selector ◽  
Electrokinetic Chromatography

Separation and determination of nimesulide related substances for quality control purposes by micellar electrokinetic chromatography

2009 ◽  
Vol 49 (2) ◽  
pp. 201-206 ◽  
Author(s):  
Constantinos K. Zacharis ◽  
Paraskevas D. Tzanavaras ◽  
Maria Notou ◽  
Anastasia Zotou ◽  
Demetrius G. Themelis
Keyword(s):  
Quality Control ◽  
Micellar Electrokinetic Chromatography ◽  
Electrokinetic Chromatography ◽  
Related Substances

scholarly journals Separation of D,L-Ampicillin by ligand exchange-micellar electrokinetic chromatography

2019 ◽  
Vol 9 (6) ◽  
pp. 4522-4533 ◽  
Keyword(s):  
Ligand Exchange ◽  
Micellar Electrokinetic Chromatography ◽  
High Sensitivity ◽  
Solvent Ratio ◽  
Electrokinetic Chromatography ◽  
Uv Detector ◽  
Chromatography Method ◽  
Pseudostationary Phase ◽  
Short Period ◽  
Applied Electrical Field

In this study, D,L-ampicillin separation was carried out by ligand exchange-micellar electrokinetic chromatography method using L-Lysine monohydrochloride as a ligand and copper (II) sulfate pentahydrate is a central ion supplier. Isomeric separations were performed using capillary electrophoresis (CE) instrument, in which SDS-L-Lys-Cu+2 micelle complex was used as a pseudostationary phase. The effect of pH, SDS amount, applied electrical field, pressure, organic solvent ratio and ampicillin D,L-ratios were investigated. Fast, inexpensive and sensitive approach for the simultaneous separation of D,L-ampicillin in both aqueous and real antibiotic sample was performed using CE coupled with UV detector. The separation was achieved in a short period of 7 minutes with high-sensitivity and low-detection limit of 1.25 μM by the developed SDS-L-Lys-Cu+2 micelle-chiral selector complexes without using any extra process such as imprinting or spacer arms for the immobilization of the ligands.

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