Simultaneous separation of sixteen positional and optical isomers of the tryptophan family by ligand-exchange micellar electrokinetic chromatography

1999 ◽  
Vol 49 (7-8) ◽  
pp. 436-443 ◽  
Author(s):  
Z. Chen ◽  
J. -M. Lin ◽  
K. Uchiyama ◽  
T. Hobo
Keyword(s):  
Ligand Exchange ◽  
Micellar Electrokinetic Chromatography ◽  
Optical Isomers ◽  
Electrokinetic Chromatography ◽  
Simultaneous Separation

scholarly journals Separation of D,L-Ampicillin by ligand exchange-micellar electrokinetic chromatography

2019 ◽  
Vol 9 (6) ◽  
pp. 4522-4533 ◽  
Keyword(s):  
Ligand Exchange ◽  
Micellar Electrokinetic Chromatography ◽  
High Sensitivity ◽  
Solvent Ratio ◽  
Electrokinetic Chromatography ◽  
Uv Detector ◽  
Chromatography Method ◽  
Pseudostationary Phase ◽  
Short Period ◽  
Applied Electrical Field

In this study, D,L-ampicillin separation was carried out by ligand exchange-micellar electrokinetic chromatography method using L-Lysine monohydrochloride as a ligand and copper (II) sulfate pentahydrate is a central ion supplier. Isomeric separations were performed using capillary electrophoresis (CE) instrument, in which SDS-L-Lys-Cu+2 micelle complex was used as a pseudostationary phase. The effect of pH, SDS amount, applied electrical field, pressure, organic solvent ratio and ampicillin D,L-ratios were investigated. Fast, inexpensive and sensitive approach for the simultaneous separation of D,L-ampicillin in both aqueous and real antibiotic sample was performed using CE coupled with UV detector. The separation was achieved in a short period of 7 minutes with high-sensitivity and low-detection limit of 1.25 μM by the developed SDS-L-Lys-Cu+2 micelle-chiral selector complexes without using any extra process such as imprinting or spacer arms for the immobilization of the ligands.

Sign in / Sign up

Export Citation Format

Share Document