ChemInform Abstract: Homoisoflavonoid Derivatives from the Roots of Ophiopogon japonicus and Their in vitro Antiinflammation Activity

ChemInform ◽  
2010 ◽  
Vol 41 (40) ◽  
pp. no-no
Author(s):  
Tran Manh Hung ◽  
Van Thu Cao ◽  
Tien Dat Nguyen ◽  
Sung-Woo Ryoo ◽  
Jeong Hyung Lee ◽  
...  
Keyword(s):  
Ophiopogon Japonicus

scholarly journals Nutritional Composition,α-Glucosidase Inhibitory and Antioxidant Activities ofOphiopogon japonicusTubers

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Yancui Wang ◽  
Feng Liu ◽  
Zongsuo Liang
Keyword(s):  
Amino Acids ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Ophiopogon Japonicus ◽  
Nutritional Compounds ◽  
Chloroform Methanol ◽  
Phenolic And Flavonoid

Ophiopogon japonicustubers have been widely used as food and traditional Chinese medicine in China. However, their nutritional composition has not been fully reported yet. This study aimed to analyze the nutritional composition ofO. japonicustubers. Theα-glucosidase inhibitory and antioxidant activities of the extracts obtained fromO. japonicustubers were also evaluated byin vitroassays. The results indicated thatO. japonicustubers are rich in carbohydrates, proteins, minerals, and amino acids. Among four extracts, the n-butanol fraction (nBF) and chloroform/methanol extract (CME) ofO. japonicustubers had high amounts of total phenolic and flavonoid contents and exhibited goodα-glucosidase inhibitory and antioxidant activities. Theα-glucosidase inhibition of nBF was higher than acarbose. Overall,O. japonicustubers are full of nutritional compounds and have goodα-glucosidase inhibitory and antioxidant activities.

Homoisoflavonoid derivatives from the roots of Ophiopogon japonicus and their in vitro anti-inflammation activity

2010 ◽  
Vol 20 (8) ◽  
pp. 2412-2416 ◽  
Author(s):  
Tran Manh Hung ◽  
Cao Van Thu ◽  
Nguyen Tien Dat ◽  
Sung-Woo Ryoo ◽  
Jeong Hyung Lee ◽  
...  
Keyword(s):  
Ophiopogon Japonicus ◽  
Anti Inflammation

Ophiopogon Saponin B Affects the Proliferation, Apoptosis, and Oxidative Stress of Osteosarcoma Cells by Regulating POU Class 3 Homeobox 3 Expression

2020 ◽  
Vol 12 (4) ◽  
pp. 518-524
Author(s):  
Zhengmao Guan ◽  
Yi Wang ◽  
Weifan Xu ◽  
Jianfeng Wu
Keyword(s):  
Oxidative Stress ◽  
Survival Rates ◽  
New Drugs ◽  
High Recurrence Rate ◽  
Steroidal Saponin ◽  
Osteosarcoma Cells ◽  
Ophiopogon Japonicus ◽  
U2os Cells ◽  
And Oxidative Stress

Osteosarcoma is characterized by early lung metastasis and a high recurrence rate. Research on anti-osteosarcoma drugs is progressing slowly, and patient survival rates have not been improved. Ophiopogon saponin B (OP-B), a steroidal saponin monomer extracted from the ophiopogon japonicus, is effective in the treatment of many cancers. We explored the mechanism underlying the in vitro effects of OP-B on proliferation, apoptosis, and oxidative stress in cultured osteosarcoma U2OS cells. We observed significant reductions in optical density, clonal number, and expression of superoxide dismutase, glutathione peroxidase, and POU Class 3 Homeobox 3 (POU3F3) in the presence of OP-B. OP-B also increased the apoptotic rate and malondialdehyde expression. Downregulation of POU3F3 inhibited U2OS proliferation and oxidative stress in osteosarcoma cells and promoted apoptosis. OP-B reduced POU3F3 expression in U2OS cells, and overexpression of POU3F3 reversed the effects of OP-B on proliferation, apoptosis, and oxidative stress. We thus conclude that OP-B promotes apoptosis and inhibits proliferation and the oxidative stress response of osteosarcoma cells by downregulating POU3F3 expression. These results suggest an avenue for the research and development of new drugs for osteosarcoma.

scholarly journals Differences in the Hemolytic Behavior of Two Isomers in Ophiopogon japonicus In Vitro and In Vivo and Their Risk Warnings

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Huan-Hua Xu ◽  
Zhen-Hong Jiang ◽  
Yu-Ting Sun ◽  
Li-Zhen Qiu ◽  
Long-Long Xu ◽  
...  
Keyword(s):  
Side Effect ◽  
Redox Balance ◽  
Underlying Mechanism ◽  
Therapeutic Effects ◽  
Proteomics Data ◽  
Mitochondrial Energy Metabolism ◽  
Ophiopogon Japonicus ◽  
Bioactive Ingredients

Ophiopogonin D (OPD) and Ophiopogonin D ′ (OPD ′ ) are two bioactive ingredients in Ophiopogon japonicus. Previously published studies have often focused on the therapeutic effects related to OPD’s antioxidant capacity but underestimated the cytotoxicity-related side effects of OPD ′ , which may result in unpredictable risks. In this study, we reported another side effect of OPD ′ , hemolysis, and what was unexpected was that this side effect also appeared with OPD. Although hemolysis effects for saponins are familiar to researchers, the hemolytic behavior of OPD or OPD ′ and the interactions between these two isomers are unique. Therefore, we investigated the effects of OPD and OPD ′ alone or in combination on the hemolytic behavior in vitro and in vivo and adopted chemical compatibility and proteomics methods to explain the potential mechanism. Meanwhile, to explain the drug-drug interactions (DDIs), molecular modeling was applied to explore the possible common targets. In this study, we reported that OPD ′ caused hemolysis both in vitro and in vivo, while OPD only caused hemolysis in vivo. We clarified the differences and DDIs in the hemolytic behavior of the two isomers. An analysis of the underlying mechanism governing this phenomenon showed that hemolysis caused by OPD or OPD ′ was related to the destruction of the redox balance of erythrocytes. In vivo, in addition to the redox imbalance, the proteomics data demonstrated that lipid metabolic disorders and mitochondrial energy metabolism are extensively involved by hemolysis. We provided a comprehensive description of the hemolysis of two isomers in Ophiopogon japonicus, and risk warnings related to hemolysis were presented. Our research also provided a positive reference for the development and further research of such bioactive components.

scholarly journals Ophiopogonin A Alleviates Hemorrhagic Shock-Induced Renal Injury via Induction of Nrf2 Expression

2021 ◽  
Vol 11 ◽  
Author(s):  
Xiaoming Sheng ◽  
Yang Yang ◽  
JiaJia Liu ◽  
Junbo Yu ◽  
Qingsong Guo ◽  
...  
Keyword(s):  
Hemorrhagic Shock ◽  
Renal Injury ◽  
Cell Model ◽  
Tunel Staining ◽  
Related Factor ◽  
Tnf Α ◽  
Wide Range ◽  
Ophiopogon Japonicus

Ophiopogonin, including Ophiopogonin A, B, C, D, is an effective active component of traditional Chinese medicine Ophiopogon japonicus which has a wide range of pharmacological effects such as protecting myocardial ischemia, resisting myocardial infarction, immune regulation, lowering blood glucose, and anti-tumor. However, the functions of ophiopogonin A on hemorrhagic shock (HS)-induced renal injury remain unclear. First, this study constructed an HS rat model and hypoxia HK-2 cell model to assess the effects of ophiopogonin A in vivo and in vitro. In vivo, HE and TUNEL staining show that ophiopogonin A dose-dependently inhibits HS-induced tissue damage and apoptosis. Moreover, ophiopogonin A dose-dependently downregulates the levels of blood urea nitrogen (BUN), creatinine (Cr), KIM-1, NGAL, iNOS, TNF-α, IL-1β, and IL-6 in HS rats kidney tissues, and decreases the number of MPO-positive cells. In vitro, we get similar results that ophiopogonin A dose-dependently improves hypoxia-induced HK-2 cell apoptosis and damage. In addition, ophiopogonin A dose-dependently increases the expression of NF E2-related factor 2 (Nrf2), while knockdown of Nrf2 reverses the functions of ophiopogonin A in vivo and in vitro. Furthermore, ophiopogonin A dose-dependently promotes the phosphorylation of ERK in HS kidney tissues and hypoxia-treated HK-2 cells, suggesting that ophiopogonin A functions via the p-ERK/ERK signaling pathway.

Role of Component Herbs in Antioxidant Activity of Shengmai San — A Traditional Chinese Medicine Formula Preventing Cerebral Oxidative Damage in Rat

2003 ◽  
Vol 31 (04) ◽  
pp. 509-521 ◽  
Author(s):  
Haruyo Ichikawa ◽  
Xuejiang Wang ◽  
Tetsuya Konishi
Keyword(s):  
Oxidative Damage ◽  
Schisandra Chinensis ◽  
Ophiopogon Japonicus ◽  
Activity Loss ◽  
Gpx Activity ◽  
Shengmai San ◽  
Tbars Formation

Traditional Chinese medicine (TCM) is an attractive model for studying antioxidant-based composite therapy. We previously reported that Shengmai San (SMS), a TCM formulation for treating cardiac disorders, inhibited cerebral oxidative damage in rats when evaluated by both glutathione peroxidase (GPX) activity loss and thiobarbituric acid reactive substance (TBARS) formation after forebrain ischemia-reperfusion. In the present study, we further examined the preventive effect of SMS and related decoctions composed of three component herbs (Panax ginseng, Ophiopogon japonicus and Schisandra chinensis) against oxidative brain injury to rationalize the complex formulation of SMS. Schisandra chinensis itself and decoctions containing it all inhibited TBARS formation in vivo. In contrast, Ophiopogon japonicus itself and formulations containing it had little effect on TBARS formation. GPX activity loss in vivo, on the other hand, was completely prevented only by SMS and Ophiopogon japonicus itself. A comparison of the in vitro antioxidant potential of SMS and related decoctions and in vivo effectiveness in preventing cerebral oxidative damage revealed that all the in vitro antioxidant indices examined here essentially correlated well with inhibition of TBARS formation in vivo. DPPH quenching and crocin bleaching activities showed particularly good correlation, and then, superoxide scavenging activity followed. However, none of them correlated with the inhibition of GPX activity loss in vivo. The role of each component herb is also discussed for the SMS effect.

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

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