Ophiopogon Saponin B Affects the Proliferation, Apoptosis, and Oxidative Stress of Osteosarcoma Cells by Regulating POU Class 3 Homeobox 3 Expression

2020 ◽  
Vol 12 (4) ◽  
pp. 518-524
Author(s):  
Zhengmao Guan ◽  
Yi Wang ◽  
Weifan Xu ◽  
Jianfeng Wu
Keyword(s):  
Oxidative Stress ◽  
Survival Rates ◽  
New Drugs ◽  
High Recurrence Rate ◽  
Steroidal Saponin ◽  
Osteosarcoma Cells ◽  
Ophiopogon Japonicus ◽  
U2os Cells ◽  
And Oxidative Stress

Osteosarcoma is characterized by early lung metastasis and a high recurrence rate. Research on anti-osteosarcoma drugs is progressing slowly, and patient survival rates have not been improved. Ophiopogon saponin B (OP-B), a steroidal saponin monomer extracted from the ophiopogon japonicus, is effective in the treatment of many cancers. We explored the mechanism underlying the in vitro effects of OP-B on proliferation, apoptosis, and oxidative stress in cultured osteosarcoma U2OS cells. We observed significant reductions in optical density, clonal number, and expression of superoxide dismutase, glutathione peroxidase, and POU Class 3 Homeobox 3 (POU3F3) in the presence of OP-B. OP-B also increased the apoptotic rate and malondialdehyde expression. Downregulation of POU3F3 inhibited U2OS proliferation and oxidative stress in osteosarcoma cells and promoted apoptosis. OP-B reduced POU3F3 expression in U2OS cells, and overexpression of POU3F3 reversed the effects of OP-B on proliferation, apoptosis, and oxidative stress. We thus conclude that OP-B promotes apoptosis and inhibits proliferation and the oxidative stress response of osteosarcoma cells by downregulating POU3F3 expression. These results suggest an avenue for the research and development of new drugs for osteosarcoma.

scholarly journals Compound Dragon’s blood capsule alleviates the degree of myocardial ischemia by improving inflammation and oxidative stress

2022 ◽  
Author(s):  
Xinkai Lyu ◽  
Xinyue Chang ◽  
Xiao Mi ◽  
Meigeng Hu ◽  
Yue Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Myocardial Ischemia ◽  
Colorimetric Method ◽  
New Drugs ◽  
H9c2 Cell ◽  
Dragon’S Blood ◽  
Tnf Α ◽  
And Oxidative Stress

Abstract Background: Compound Dragon's blood capsule (CDC) is a patent medicine mainly composed of dragon’s blood (Dracaena cochinchinensis (Lour.) S. C. Chen), notoginseng (Parmx notoginseng (Burk.) F. H. Chen) and borneol (C10H18O) for the treatment of stabilize coronary heart disease (CHD) and myocardial ischemia (MI). This paper is to investigate the anti-myocardial ischemia properties of CDC both in vivo and vitro.Methods: The fingerprint of CDC was established by UPLC-Q/TOF-MS. The hypoxia/reoxygenation (H/R) model was established by using H9c2 cells. The levels of LDH, SOD and MDA were detected by colorimetric method. Moreover, the MI model of rats was established by isoprenaline hydrochloride (ISO), the mortality rate was recorded, the changes in J point of electrocardiogram were determined, the expressions of the myocardial markers, oxidative stress markers (CK, CK-MB, LDH and SOD) and inflammatory mediators (TNF-α, IL-6, IL-10, IL-1β and NO) in serum were detected. Results: The fingerprint of CDC was established and 10 mainly active components were identified: 7,4'-dihydroxyflavone, resveratrol, loureirin A, loureirin B, pterostilbene were identified from Dragon's blood, notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, oleanolic acid, ginsenoside Rd were identified from notoginseng. In vitro study, CDC significantly improved H9c2 cell viability and SOD level (P < 0.05), decreased LDH and MDA level (P < 0.05). In vivo study, CDC increased survival rate and SOD level of serum, decreased J-point of ECG, CK-MB, LDH, TNF-α, IL-6, IL-10 level (P < 0.05).Conclusions: CDC had a significant anti-myocardial ischemia effect by alleviating inflammation and oxidative stress, suggesting that CDC is a suitable adjuvent to treat CHD, dragon’s blood has the prospect of developing other new drugs.

scholarly journals Impact of Polyphenolic-Food on Longevity: An Elixir of Life. An Overview

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 507
Author(s):  
Rosaria Meccariello ◽  
Stefania D’Angelo
Keyword(s):  
Oxidative Stress ◽  
Food Sources ◽  
Preventive Action ◽  
Nutritional Interventions ◽  
Beneficial Effects ◽  
Age Related ◽  
Macromolecular Damage ◽  
And Oxidative Stress

Aging and, particularly, the onset of age-related diseases are associated with tissue dysfunction and macromolecular damage, some of which can be attributed to accumulation of oxidative damage. Recently, growing interest has emerged on the beneficial effects of plant-based diets for the prevention of chronic diseases including obesity, diabetes, and cardiovascular disease. Several studies collectively suggests that the intake of polyphenols and their major food sources may exert beneficial effects on improving insulin resistance and related diabetes risk factors, such as inflammation and oxidative stress. They are the most abundant antioxidants in the diet, and their intake has been associated with a reduced aging in humans. Polyphenolic intake has been shown to be effective at ameliorating several age-related phenotypes, including oxidative stress, inflammation, impaired proteostasis, and cellular senescence, both in vitro and in vivo. In this paper, effects of these phytochemicals (either pure forms or polyphenolic-food) are reviewed and summarized according to affected cellular signaling pathways. Finally, the effectiveness of the anti-aging preventive action of nutritional interventions based on diets rich in polyphenolic food, such as the diets of the Blue zones, are discussed.

Pirfenidone mediates cigarette smoke extract induced inflammation and oxidative stress in vitro and in vivo

2021 ◽  
Vol 96 ◽  
pp. 107593
Author(s):  
Yiming Ma ◽  
Lijuan Luo ◽  
Xiangming Liu ◽  
Herui Li ◽  
Zihang Zeng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cigarette Smoke ◽  
Cigarette Smoke Extract ◽  
And Oxidative Stress

In vitro study on the effects of iron sucrose, ferric gluconate and iron dextran on redox-active iron and oxidative stress

2011 ◽  
Vol 60 (07) ◽  
pp. 459-465
Author(s):  
Brigitte Sturm ◽  
Hannes Steinkellner ◽  
Nina Ternes ◽  
Hans Goldenberg ◽  
Barbara Scheiber-Mojdehkar
Keyword(s):  
Oxidative Stress ◽  
In Vitro Study ◽  
Iron Sucrose ◽  
Vitro Study ◽  
Iron Dextran ◽  
Active Iron ◽  
Redox Active ◽  
And Oxidative Stress

The protective effects of carvacrol and thymol against paracetamol–induced toxicity on human hepatocellular carcinoma cell lines (HepG2)

2016 ◽  
Vol 35 (12) ◽  
pp. 1252-1263 ◽  
Author(s):  
SS Palabiyik ◽  
E Karakus ◽  
Z Halici ◽  
E Cadirci ◽  
Y Bayir ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Cytokines ◽  
Hepg2 Cells ◽  
Folk Medicine ◽  
Hepatoprotective Activity ◽  
High Dose ◽  
Protective Effects ◽  
And Oxidative Stress ◽  
Pro Inflammatory Cytokines

Acetaminophen (APAP) overdose could induce liver damage and lead to acute liver failure. The treatment of APAP overdoses could be improved by new therapeutic strategies. Thymus spp., which has many beneficial effects and has been used in folk medicine, is one such potential strategy. In the present study, the hepatoprotective activity of the main constituents of Thymus spp., carvacrol and thymol, were evaluated in light of APAP-induced hepatotoxicity. We hoped to understand the hepatoprotective mechanism of these agents on the antioxidant system and pro-inflammatory cytokines in vitro. Dose-dependent effects of thymol and carvacrol (25, 50, and 100 µM) were tested on cultured HepG2 cells. N-Acetylcysteine (NAC) was tested as positive control. We showed that APAP inhibited HepG2 cell growth by inducing inflammation and oxidative stress. Incubating APAP-exposed HepG2 cells with carvacrol and thymol for 24 h ameliorated this inflammation and oxidative stress. We also evaluated alanine transaminase and lactate dehydrogenase levels of HepG2 cells. We found that thymol and carvacrol protected against APAP-induced toxicity in HepG2 cells by increasing antioxidant activity and reducing pro-inflammatory cytokines, such as tumor necrosis factor α and interleukin 1β. Taking together high-dose thymol and carvacrol treatment has an effect close to NAC treatment in APAP toxicity, but thymol has better treatment effect than carvacrol.

Diosgenin, a steroidal saponin, prevents hypertension, cardiac remodeling and oxidative stress in adenine induced chronic renal failure rats

RSC Advances ◽  
2015 ◽  
Vol 5 (25) ◽  
pp. 19337-19344 ◽  
Author(s):  
Jeganathan Manivannan ◽  
Janakiraman Shanthakumar ◽  
Thangarasu Silambarasan ◽  
Elumalai Balamurugan ◽  
Boobalan Raja
Keyword(s):  
Oxidative Stress ◽  
Renal Failure ◽  
Chronic Renal Failure ◽  
Cardiac Remodeling ◽  
Steroidal Saponin ◽  
Prevention Of Hypertension ◽  
And Oxidative Stress

Prevention of hypertension, cardiac remodeling and oxidative stress in chronic renal failure (CRF) rats by diosgenin.

scholarly journals Coenzyme Q10, Ageing and the Nervous System: An Overview

Antioxidants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 2
Author(s):  
David Mantle ◽  
Robert A. Heaton ◽  
Iain P. Hargreaves
Keyword(s):  
Oxidative Stress ◽  
Coenzyme Q10 ◽  
Neurological Disorders ◽  
Neurological Disease ◽  
Age Related ◽  
Increased Risk ◽  
Potential Therapeutic Role ◽  
Ageing Brain ◽  
And Oxidative Stress

The ageing brain is characterised by changes at the physical, histological, biochemical and physiological levels. This ageing process is associated with an increased risk of developing a number of neurological disorders, notably Alzheimer’s disease and Parkinson’s disease. There is evidence that mitochondrial dysfunction and oxidative stress play a key role in the pathogenesis of such disorders. In this article, we review the potential therapeutic role in these age-related neurological disorders of supplementary coenzyme Q10, a vitamin-like substance of vital importance for normal mitochondrial function and as an antioxidant. This review is concerned primarily with studies in humans rather than in vitro studies or studies in animal models of neurological disease. In particular, the reasons why the outcomes of clinical trials supplementing coenzyme Q10 in these neurological disorders is discussed.

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