Red-Shifted Fluorescent Aminated Derivatives of a Conformationally Locked GFP Chromophore

2014 ◽  
Vol 20 (41) ◽  
pp. 13234-13241 ◽  
Author(s):  
Mikhail S. Baranov ◽  
Kyril M. Solntsev ◽  
Nadezhda S. Baleeva ◽  
Alexander S. Mishin ◽  
Sergey A. Lukyanov ◽  
...  
2019 ◽  
Vol 60 (34) ◽  
pp. 150963 ◽  
Author(s):  
Nadezhda S. Baleeva ◽  
Anastasia V. Khavroshechkina ◽  
Elvira R. Zaitseva ◽  
Ivan N. Myasnyanko ◽  
Marina B. Zagudaylova ◽  
...  

2018 ◽  
Vol 19 (12) ◽  
pp. 3778 ◽  
Author(s):  
Nina Bozhanova ◽  
Mikhail Baranov ◽  
Nadezhda Baleeva ◽  
Alexey Gavrikov ◽  
Alexander Mishin

Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs.


2003 ◽  
Vol 85 (3) ◽  
pp. 1839-1850 ◽  
Author(s):  
Anny Follenius-Wund ◽  
Maryline Bourotte ◽  
Martine Schmitt ◽  
Fatih Iyice ◽  
Hans Lami ◽  
...  

2005 ◽  
Vol 70 (1) ◽  
pp. 103-123 ◽  
Author(s):  
Hubert Hřebabecký ◽  
Milena Masojídková ◽  
Antonín Holý

(1R*,3R*,6R*,7S*,9S*)- and (1R*,3R*,6R*,7S*,9R*)-9-Amino-5-oxatricyclo[4.2.1.03,7]nonane-3-methanols (16aand17a) were prepared from 2-(hydroxymethyl)bicyclo[2.2.1]hept-5-ene-2-methanol (10) in five easy steps. The amines16aand17awere used to construct 6-chloro-9H-purine20and21, 2-amino-6-chloro-9H-purine30and31, and 6-chloro-8-methyl-9H-purine analogues34and35. Ammonolysis of these compounds led to 6-amino-9H-purine22aand23a, 2,6-diamino-9H-purine32and33, and 6-amino-8-methyl-9H-purine derivatives of 5-oxatricyclo[4.2.1.03,7]nonane-3-methanol36and37. (1R*,3R*,6R*,7S*,9S*)- and (1R*,3R*,6R*,7S*,9R*)-9-[6-(Dimethylamino)-9H-purin-9-yl]-5-oxatricyclo[4.2.1.03,7]nonane-3-methanols (22band23b), and (1R*,3R*,6R*,7S*,9S*)- and (1R*,3R*,6R*,7S*,9R*)-9-[6-(cyclopropylamino)-9H-purin-9-yl]-5-oxatricyclo[4.2.1.03,7]nonane-3-methanols (22cand23c) were prepared by aminolysis of20and21.


2016 ◽  
Vol 18 (38) ◽  
pp. 26703-26711 ◽  
Author(s):  
Seth Olsen ◽  
Mikhail S. Baranov ◽  
Nadezhda S. Baleeva ◽  
Maria M. Antonova ◽  
Kenneth A. Johnson ◽  
...  

We report the synthesis and characterization of a pH-sensitive fluorescence switch based on a conformationally-locked green fluorescent protein (GFP) chromophore.


RSC Advances ◽  
2012 ◽  
Vol 2 (22) ◽  
pp. 8243 ◽  
Author(s):  
Mikkel Andreas Christensen ◽  
Karsten Jennum ◽  
Peter Bæch Abrahamsen ◽  
Eduardo Antonio Della Pia ◽  
Kasper Lincke ◽  
...  

2017 ◽  
Vol 2017 (35) ◽  
pp. 5219-5224 ◽  
Author(s):  
Nadezhda S. Baleeva ◽  
Snezhana O. Zaitseva ◽  
Dmitriy A. Gorbachev ◽  
Alexander Yu. Smirnov ◽  
Marina B. Zagudaylova ◽  
...  

2020 ◽  
Author(s):  
Vladislav R. Aslopovsky ◽  
Aleksander M. Shakhov ◽  
Artyom A. Astafiev ◽  
Vladimir E. Bochenkov

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