pH-Sensitive fluorophores from locked GFP chromophores by a non-alternant analogue of the photochemical meta effect

2016 ◽  
Vol 18 (38) ◽  
pp. 26703-26711 ◽  
Author(s):  
Seth Olsen ◽  
Mikhail S. Baranov ◽  
Nadezhda S. Baleeva ◽  
Maria M. Antonova ◽  
Kenneth A. Johnson ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Ph Sensitive ◽  
Synthesis And Characterization ◽  
Fluorescence Switch ◽  
Gfp Chromophore ◽  
Green Fluorescent ◽  
Conformationally Locked

We report the synthesis and characterization of a pH-sensitive fluorescence switch based on a conformationally-locked green fluorescent protein (GFP) chromophore.

scholarly journals The yeast peroxisomal adenine nucleotide transporter: characterization of two transport modes and involvement in ΔpH formation across peroxisomal membranes

2004 ◽  
Vol 381 (3) ◽  
pp. 581-585 ◽  
Author(s):  
Francesco M. LASORSA ◽  
Pasquale SCARCIA ◽  
Ralf ERDMANN ◽  
Ferdinando PALMIERI ◽  
Hanspeter ROTTENSTEINER ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Imaging ◽  
Fluorescent Protein ◽  
Adenine Nucleotide ◽  
Ph Sensitive ◽  
Peroxisomal Membrane ◽  
Transport Modes ◽  
Proton Movement ◽  
Green Fluorescent

The yeast peroxisomal adenine nucleotide carrier, Ant1p, was shown to catalyse unidirectional transport in addition to exchange of substrates. In both transport modes, proton movement occurs. Nucleotide hetero-exchange is H+-compensated and electroneutral. Furthermore, microscopic fluorescence imaging of a pH-sensitive green fluorescent protein targeted to peroxisomes shows that Ant1p is involved in the formation of a ΔpH across the peroxisomal membrane, acidic inside.

Synthesis and Characterization of Model Compounds for the Neutral Green Fluorescent Protein Chromophore

Synthesis ◽  
2007 ◽  
Vol 2007 (23) ◽  
pp. 3635-3638 ◽  
Author(s):  
Mogens Nielsen ◽  
Michael Petersen ◽  
Peter Riber ◽  
Lars Andersen
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Synthesis And Characterization ◽  
Model Compounds ◽  
Green Fluorescent

Fluorescent and “breathable” CO2 responsive vesicles inspired from green fluorescent protein

2017 ◽  
Vol 8 (40) ◽  
pp. 6283-6288 ◽  
Author(s):  
Lei Xu ◽  
Ning Ren ◽  
Ji Pang ◽  
Hongping Deng ◽  
Xinyuan Zhu ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Block Copolymer ◽  
Fluorescent Protein ◽  
Gfp Chromophore ◽  
Green Fluorescent

CO2 responsive fluorescent vesicles from a GFP chromophore labeled block-copolymer could change their size and fluorescence to mimic jellyfish breathing.

scholarly journals Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins

2018 ◽  
Vol 19 (12) ◽  
pp. 3778 ◽  
Author(s):  
Nina Bozhanova ◽  
Mikhail Baranov ◽  
Nadezhda Baleeva ◽  
Alexey Gavrikov ◽  
Alexander Mishin
Keyword(s):  
Green Fluorescent Protein ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Protein Labeling ◽  
Cell Protein ◽  
Gfp Chromophore ◽  
Green Fluorescent ◽  
Derivatives Of

Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs.

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