Signal-Off and Signal-On Design for a Label-Free Aptasensor Based on Target-Induced Self-Assembly and Abasic-Site-Binding Ligands

2009 ◽  
Vol 15 (40) ◽  
pp. 10375-10378 ◽  
Author(s):  
Zhiai Xu ◽  
Yusuke Sato ◽  
Seiichi Nishizawa ◽  
Norio Teramae
Keyword(s):  
Self Assembly ◽  
Label Free ◽  
Abasic Site ◽  
Site Binding

Simple label-free fluorescence detection of apurinic/apyrimidinic endonuclease 1 activity and its inhibitor using the abasic site-binding fluorophore

2019 ◽  
Vol 11 (6) ◽  
pp. 739-743 ◽  
Author(s):  
Xuejun Li ◽  
Mei Xiong ◽  
Yufeng Huang ◽  
Liangliang Zhang ◽  
Shulin Zhao
Keyword(s):  
Fluorescence Detection ◽  
Detection Method ◽  
Label Free ◽  
Abasic Site ◽  
Site Binding

A label-free fluorescence detection method for apurinic/apyrimidinic endonuclease 1 activity was reported based on the abasic site-binding fluorophore.

scholarly journals Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
Han Yan ◽  
Zhen Zhang ◽  
Ting Weng ◽  
Libo Zhu ◽  
Pang Zhang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Detection Method ◽  
Logic Gate ◽  
Detection Methods ◽  
Label Free ◽  
Recognition Method ◽  
Operation Pattern ◽  
Simple Detection ◽  
Dna Tetrahedron ◽  
Marker Molecule

Nanopores have a unique advantage for detecting biomolecules in a label-free fashion, such as DNA that can be synthesized into specific structures to perform computations. This method has been considered for the detection of diseased molecules. Here, we propose a novel marker molecule detection method based on DNA logic gate by deciphering a variable DNA tetrahedron structure using a nanopore. We designed two types of probes containing a tetrahedron and a single-strand DNA tail which paired with different parts of the target molecule. In the presence of the target, the two probes formed a double tetrahedron structure. As translocation of the single and the double tetrahedron structures under bias voltage produced different blockage signals, the events could be assigned into four different operations, i.e., (0, 0), (0, 1), (1, 0), (1, 1), according to the predefined structure by logic gate. The pattern signal produced by the AND operation is obviously different from the signal of the other three operations. This pattern recognition method has been differentiated from simple detection methods based on DNA self-assembly and nanopore technologies.

Layer-by-layer self-assembly of 2D graphene nanosheets, 3D copper oxide nanoflowers and 0D gold nanoparticles for ultrasensitive electrochemical detection of alpha fetoprotein

RSC Advances ◽  
2015 ◽  
Vol 5 (70) ◽  
pp. 56583-56589 ◽  
Author(s):  
Yulan Wang ◽  
Dan Wu ◽  
Yong Zhang ◽  
Xiang Ren ◽  
Yaoguang Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Copper Oxide ◽  
Electrochemical Detection ◽  
Self Assembly ◽  
Graphene Nanosheets ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Quantitative Detection ◽  
Layer By Layer ◽  
Label Free

In this work, a novel and ultrasensitive label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP).

scholarly journals Comparison of DNAzyme activity for the development of an immobilized heme sensor

MRS Advances ◽  
2018 ◽  
Vol 3 (26) ◽  
pp. 1491-1496
Author(s):  
Natalie Hughes ◽  
Nancy Nguyen ◽  
Deanna-Kaye Daley ◽  
Justin Grennell ◽  
Amira Gee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Label Free ◽  
Detection Range ◽  
Chemical Complexity ◽  
Detection Strategy ◽  
Detection Approach ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Care Systems

ABSTRACTPoint-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing

2013 ◽  
Vol 19 (38) ◽  
pp. 12846-12852 ◽  
Author(s):  
Yan Wang ◽  
Jian Chen ◽  
Huping Jiao ◽  
Yang Chen ◽  
Wenying Li ◽  
...  
Keyword(s):  
Self Assembly ◽  
Label Free ◽  
Turn On ◽  
New Strategy ◽  
Fluorescence Turn On

A novel label-free fluorescence assay for dipeptidyl peptidase 4 activity detection based on supramolecular self-assembly

2020 ◽  
Vol 56 (11) ◽  
pp. 1629-1632
Author(s):  
Yu Zhao ◽  
Jiawen Zou ◽  
Yanyan Song ◽  
Jun Peng ◽  
Yi Wang ◽  
...  
Keyword(s):  
Biological Samples ◽  
Self Assembly ◽  
Dipeptidyl Peptidase ◽  
Fluorescence Assay ◽  
Supramolecular System ◽  
Label Free ◽  
Activity Detection ◽  
Inhibitor Screening ◽  
Dipeptidyl Peptidase 4

A novel supramolecular system CB[7]-GPFG for dipeptidyl peptidase 4 (DPP4) activity detection in biological samples and inhibitor screening.

Label-free and Real-Time Sequence Specific DNA Detection Based on Supramolecular Self-assembly

Langmuir ◽  
2010 ◽  
Vol 26 (9) ◽  
pp. 6832-6837 ◽  
Author(s):  
Yanli Tang ◽  
Komandoor E. Achyuthan ◽  
David G. Whitten
Keyword(s):  
Real Time ◽  
Self Assembly ◽  
Dna Detection ◽  
Time Sequence ◽  
Label Free
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