Fabrication and Modeling of an Ultrasensitive Label Free Impedimetric Immunosensor for Aflatoxin B1 based on Protein A Self-assembly Modified Gold 3D Nanotube Electrode ensembles

2017 ◽  
Vol 247 ◽  
pp. 1052-1059 ◽  
Author(s):  
Xiaolong Li ◽  
Lixin Cao ◽  
Yuejuan Zhang ◽  
Peisheng Yan ◽  
Donald W. Kirk
Keyword(s):  
Aflatoxin B1 ◽  
Self Assembly ◽  
Protein A ◽  
Label Free ◽  
Impedimetric Immunosensor

Study on the Novel Label Free Impedimetric Immunosensor for Aflatoxin B1 Based on Poly(o-phenylenediamine) Film Modified Gold Electrode

2011 ◽  
Vol 322 ◽  
pp. 385-389 ◽  
Author(s):  
Ning Ning Wu ◽  
Li Xin Cao ◽  
Pei Sheng Yan ◽  
Ming Hao Wang
Keyword(s):  
Aflatoxin B1 ◽  
Gold Electrode ◽  
Biomolecular Recognition ◽  
The Novel ◽  
Label Free ◽  
Buffer Solution ◽  
Solution Ph ◽  
Linear Detection ◽  
Impedimetric Immunosensor

A label free impedimetric immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing anti-AFB1 onto Poly o-phenylenediamine (PoPD) electropolymerized film modified gold electrode by glutaraldehyde (GA) cross-linking. An electrochemical interfacial modeling of biomolecular recognition was recommended and reasonably interpreted. EIS technology was employed for the quantitative determination. The linear detection concentration ranges of AFB1 were 0.03~0.1 μg/mL and 0.1~0.7 μg/mL. The detection limit was 2.8×10-8μg/mL. The immunosensor could be reused more than 10 times when renewed by HCl-Glycine buffer solution (pH 2.6).

scholarly journals Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
Han Yan ◽  
Zhen Zhang ◽  
Ting Weng ◽  
Libo Zhu ◽  
Pang Zhang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Detection Method ◽  
Logic Gate ◽  
Detection Methods ◽  
Label Free ◽  
Recognition Method ◽  
Operation Pattern ◽  
Simple Detection ◽  
Dna Tetrahedron ◽  
Marker Molecule

Nanopores have a unique advantage for detecting biomolecules in a label-free fashion, such as DNA that can be synthesized into specific structures to perform computations. This method has been considered for the detection of diseased molecules. Here, we propose a novel marker molecule detection method based on DNA logic gate by deciphering a variable DNA tetrahedron structure using a nanopore. We designed two types of probes containing a tetrahedron and a single-strand DNA tail which paired with different parts of the target molecule. In the presence of the target, the two probes formed a double tetrahedron structure. As translocation of the single and the double tetrahedron structures under bias voltage produced different blockage signals, the events could be assigned into four different operations, i.e., (0, 0), (0, 1), (1, 0), (1, 1), according to the predefined structure by logic gate. The pattern signal produced by the AND operation is obviously different from the signal of the other three operations. This pattern recognition method has been differentiated from simple detection methods based on DNA self-assembly and nanopore technologies.

A label-free aptasensor based on a dual-emission fluorescent strategy for aflatoxin B1 detection

2021 ◽  
pp. 130561
Author(s):  
Yao-Yao Fan ◽  
Jun Li ◽  
Li Fan ◽  
Jie Wen ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Label Free ◽  
Dual Emission

Layer-by-layer self-assembly of 2D graphene nanosheets, 3D copper oxide nanoflowers and 0D gold nanoparticles for ultrasensitive electrochemical detection of alpha fetoprotein

RSC Advances ◽  
2015 ◽  
Vol 5 (70) ◽  
pp. 56583-56589 ◽  
Author(s):  
Yulan Wang ◽  
Dan Wu ◽  
Yong Zhang ◽  
Xiang Ren ◽  
Yaoguang Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Copper Oxide ◽  
Electrochemical Detection ◽  
Self Assembly ◽  
Graphene Nanosheets ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Quantitative Detection ◽  
Layer By Layer ◽  
Label Free

In this work, a novel and ultrasensitive label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP).

Construction of an impedimetric immunosensor for label-free detecting carbofuran residual in agricultural and environmental samples

Food Control ◽  
2015 ◽  
Vol 53 ◽  
pp. 72-80 ◽  
Author(s):  
Lin Liu ◽  
Dan Xu ◽  
Yinyue Hu ◽  
Shuzhao Liu ◽  
Honglin Wei ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Label Free ◽  
Impedimetric Immunosensor

scholarly journals Comparison of DNAzyme activity for the development of an immobilized heme sensor

MRS Advances ◽  
2018 ◽  
Vol 3 (26) ◽  
pp. 1491-1496
Author(s):  
Natalie Hughes ◽  
Nancy Nguyen ◽  
Deanna-Kaye Daley ◽  
Justin Grennell ◽  
Amira Gee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Label Free ◽  
Detection Range ◽  
Chemical Complexity ◽  
Detection Strategy ◽  
Detection Approach ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Care Systems

ABSTRACTPoint-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

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