A novel label-free fluorescence assay for dipeptidyl peptidase 4 activity detection based on supramolecular self-assembly

2020 ◽  
Vol 56 (11) ◽  
pp. 1629-1632
Author(s):  
Yu Zhao ◽  
Jiawen Zou ◽  
Yanyan Song ◽  
Jun Peng ◽  
Yi Wang ◽  
...  
Keyword(s):  
Biological Samples ◽  
Self Assembly ◽  
Dipeptidyl Peptidase ◽  
Fluorescence Assay ◽  
Supramolecular System ◽  
Label Free ◽  
Activity Detection ◽  
Inhibitor Screening ◽  
Dipeptidyl Peptidase 4

A novel supramolecular system CB[7]-GPFG for dipeptidyl peptidase 4 (DPP4) activity detection in biological samples and inhibitor screening.

scholarly journals A label-free and sensitive fluorescence assay for hyaluronidase activity through electrostatic-controlled quantum dots self-assembly

2021 ◽  
pp. 174751982110189
Author(s):  
Yan Wang ◽  
Wenwen Gao
Keyword(s):  
Quantum Dots ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Complex Mixture ◽  
High Sensitivity ◽  
Fluorescence Assay ◽  
Label Free ◽  
Activity Detection ◽  
Sensitivity And Selectivity ◽  
Mixture Sample

A label-free fluorescence assay for hyaluronidase (HAase) activity based on self-assembly of quantum dots is developed. A cationic polymer (polycation) can induce aggregation of the negatively charged quantum dots through electrostatic interactions and the fluorescence of the quantum dots is quenched. When the polycation is mixed with hyaluronic acid (HA), intense binding of HA to the polycation makes the quantum dots free and recovery of the fluorescence of the quantum dots is observed. However, in the presence of HAase, HA is hydrolyzed into small fragments and the polycation induces reaggregation of the quantum dots. A simple and rapid fluorescence sensor with high sensitivity and selectivity for HAase activity detection is therefore successfully established with a detection limit of 0.01 U/mL. Moreover, we have demonstrated an assay that can be applied to detect HAase activity in a complex mixture sample including 1% human serum.

Label-free fluorescence assay based on near-infrared B,N-doped carbon dots as a fluorescent probe for the detection of sialic acid

2020 ◽  
Vol 44 (6) ◽  
pp. 2350-2356 ◽  
Author(s):  
Nan Wang ◽  
Mengke Wang ◽  
Yang Yu ◽  
Guojian Yang ◽  
Xingguang Su
Keyword(s):  
Sialic Acid ◽  
Fluorescent Probe ◽  
Carbon Dots ◽  
Near Infrared ◽  
Fluorescence Assay ◽  
Label Free ◽  
Activity Detection ◽  
Doped Carbon Dots ◽  
Co Doped

A simple and sensitive sensing strategy for sialic acid activity detection on the basis of novel near-infrared B,N co-doped carbon dots was constructed.

A Time-Resolved Fluorescence Probe for Dipeptidyl Peptidase 4 and Its Application in Inhibitor Screening

2010 ◽  
Vol 16 (45) ◽  
pp. 13479-13486 ◽  
Author(s):  
Mitsuyasu Kawaguchi ◽  
Takayoshi Okabe ◽  
Takuya Terai ◽  
Kenjiro Hanaoka ◽  
Hirotatsu Kojima ◽  
...  
Keyword(s):  
Fluorescence Probe ◽  
Dipeptidyl Peptidase ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Inhibitor Screening ◽  
Dipeptidyl Peptidase 4
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