Front Cover: Redirection of miRNA‐Argonaute Complexes to Specific Target Sites by Synthetic Adaptor Molecules (C&B 7/2020)

2020 ◽  
Vol 17 (7) ◽  
Author(s):  
Mathias Bolz ◽  
Laura Thomas ◽  
Ute Scheffer ◽  
Elisabeth Kalden ◽  
Roland K. Hartmann ◽  
...  
Keyword(s):  
Front Cover ◽  
Specific Target ◽  
Target Sites

scholarly journals SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Santosh Kumar Upadhyay ◽  
Shailesh Sharma
Keyword(s):  
Operating Systems ◽  
High Throughput ◽  
Genomic Data ◽  
Prediction Tool ◽  
High Demand ◽  
Specific Target ◽  
Target Sites ◽  
High Throughput Detection ◽  
User Friendly

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained challenging. Here, we report SSFinder, a python script to perform high throughput detection of specific target sites in large nucleotide datasets. The SSFinder is a user-friendly tool, compatible with Windows, Mac OS, and Linux operating systems, and freely available online.

scholarly journals Demonstration of Conjugative Transposon (Tn5397)-Mediated Horizontal Gene Transfer between Clostridium difficile and Enterococcus faecalis

2010 ◽  
Vol 54 (11) ◽  
pp. 4924-4926 ◽  
Author(s):  
Azmiza S. Jasni ◽  
Peter Mullany ◽  
Haitham Hussain ◽  
Adam P. Roberts
Keyword(s):  
Gene Transfer ◽  
Clostridium Difficile ◽  
Horizontal Gene Transfer ◽  
Enterococcus Faecalis ◽  
Nosocomial Infections ◽  
Conjugative Transposon ◽  
Antibiotic Resistant ◽  
Specific Target ◽  
Ribotype 027 ◽  
Target Sites

ABSTRACT Antibiotic-resistant Enterococcus faecalis and Clostridium difficile are responsible for nosocomial infections in humans, in which they inhabit the same niche. Here, we demonstrate transfer of the conjugative transposon Tn5397 from C. difficile 630 to E. faecalis JH2-2, the first reported gene transfer between these two bacteria. Furthermore, transfer from the E. faecalis EF20A transconjugant to the epidemic ribotype 027 C. difficile strain R20291 was also demonstrated. Tn5397 was shown to use a single specific target site in E. faecalis; it also has specific target sites in C. difficile. These experiments highlight the importance of continual monitoring for emerging resistances in these bacteria.

scholarly journals The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid attaches covalently to DNA via a phosphotyrosyl linkage.

1985 ◽  
Vol 5 (11) ◽  
pp. 3274-3279 ◽  
Author(s):  
R M Gronostajski ◽  
P D Sadowski
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Sequences ◽  
Flp Recombinase ◽  
Specific Target ◽  
Target Sites ◽  
Dna Strands ◽  
2 Micron Plasmid ◽  
Dna Strand

The FLP recombinase, encoded by the 2 micron plasmid of Saccharomyces cerevisiae, promotes efficient recombination in vivo and in vitro between its specific target sites (FLP sites). It was previously determined that FLP interacts with DNA sequences within its target site (B. J. Andrews, G. A. Proteau, L. G. Beatty, and P. D. Sadowski. Cell 40:795-803, 1985), generates a single-stranded break on both DNA strands within the FLP site, and remains covalently attached to the 3' end of each break. We now show that the FLP protein is bound to the 3' side of each break by an O-phosphotyrosyl residue and that it appears that the same tyrosyl residue(s) is used to attach to either DNA strand within the FLP site.

scholarly journals FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes

1997 ◽  
Vol 25 (18) ◽  
pp. 3665-3671 ◽  
Author(s):  
M. M. Golic ◽  
Y. S. Rong ◽  
R. B. Petersen ◽  
S. L. Lindquist ◽  
K. G. Golic
Keyword(s):  
Specific Target ◽  
Target Sites

The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid attaches covalently to DNA via a phosphotyrosyl linkage

1985 ◽  
Vol 5 (11) ◽  
pp. 3274-3279
Author(s):  
R M Gronostajski ◽  
P D Sadowski
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Sequences ◽  
Flp Recombinase ◽  
Specific Target ◽  
Target Sites ◽  
Dna Strands ◽  
2 Micron Plasmid ◽  
Dna Strand

The FLP recombinase, encoded by the 2 micron plasmid of Saccharomyces cerevisiae, promotes efficient recombination in vivo and in vitro between its specific target sites (FLP sites). It was previously determined that FLP interacts with DNA sequences within its target site (B. J. Andrews, G. A. Proteau, L. G. Beatty, and P. D. Sadowski. Cell 40:795-803, 1985), generates a single-stranded break on both DNA strands within the FLP site, and remains covalently attached to the 3' end of each break. We now show that the FLP protein is bound to the 3' side of each break by an O-phosphotyrosyl residue and that it appears that the same tyrosyl residue(s) is used to attach to either DNA strand within the FLP site.

scholarly journals Topical and Transdermal Drug Delivery: From Simple Potions to Smart Technologies

2019 ◽  
Vol 16 (5) ◽  
pp. 444-460 ◽  
Author(s):  
Heather A.E. Benson ◽  
Jeffrey E. Grice ◽  
Yousuf Mohammed ◽  
Sarika Namjoshi ◽  
Michael S. Roberts
Keyword(s):  
Drug Delivery ◽  
Transdermal Drug Delivery ◽  
Percutaneous Absorption ◽  
Systemic Effects ◽  
Specific Target ◽  
Anatomy And Physiology ◽  
Skin Delivery ◽  
Target Sites ◽  
Ancient Times ◽  
Smart Technologies

This overview on skin delivery considers the evolution of the principles of percutaneous absorption and skin products from ancient times to today. Over the ages, it has been recognised that products may be applied to the skin for either local or systemic effects. As our understanding of the anatomy and physiology of the skin has improved, this has facilitated the development of technologies to effectively and quantitatively deliver solutes across this barrier to specific target sites in the skin and beyond. We focus on these technologies and their role in skin delivery today and in the future.

scholarly journals Long-range, selective, on-demand suspension interactions: Combining and triggering soluto-inertial beacons

2019 ◽  
Vol 5 (8) ◽  
pp. eaax1893 ◽  
Author(s):  
Anirudha Banerjee ◽  
Todd M. Squires
Keyword(s):  
Physicochemical Properties ◽  
Long Range ◽  
Particle Migration ◽  
Specific Target ◽  
On Demand ◽  
Solute Fluxes ◽  
Target Sites ◽  
Si Effect ◽  
Migration Velocities ◽  
Particle Delivery

Structures and particles that slowly release solute into solution can attract or repel other particles in suspension via diffusiophoresis, a process we termed “soluto-inertial (SI) interactions.” These SI interactions involve “beacons” that establish and sustain nonequilibrium solute fluxes over long durations. Here, we demonstrate the versatility of the SI concept and introduce distinct strategies to manipulate solute gradients and, hence, suspension behavior using beacons with different physicochemical properties. First, we demonstrate on-demand particle migration using beacons that can be actuated with a trigger. We then show the synergy between multiple, distinct beacons that modify solute fluxes in a way that allows directed, yet selective, colloidal migration to specific target sites. Moreover, this multibeacon harmony enhances migration velocities, and delays the equilibration of the SI effect. The different SI techniques highlighted here suggest previously unidentified possibilities for sorting and separating colloidal mixtures, targeting particle delivery, and enhancing rates of suspension flocculation.

scholarly journals Redirection of miRNA‐Argonaute Complexes to Specific Target Sites by Synthetic Adaptor Molecules

2020 ◽  
Vol 17 (7) ◽  
Author(s):  
Mathias Bolz ◽  
Laura Thomas ◽  
Ute Scheffer ◽  
Elisabeth Kalden ◽  
Roland K. Hartmann ◽  
...  
Keyword(s):  
Specific Target ◽  
Target Sites
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