scholarly journals SSFinder: High Throughput CRISPR-Cas Target Sites Prediction Tool

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Santosh Kumar Upadhyay ◽  
Shailesh Sharma
Keyword(s):  
Operating Systems ◽  
High Throughput ◽  
Genomic Data ◽  
Prediction Tool ◽  
High Demand ◽  
Specific Target ◽  
Target Sites ◽  
High Throughput Detection ◽  
User Friendly

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system facilitates targeted genome editing in organisms. Despite high demand of this system, finding a reliable tool for the determination of specific target sites in large genomic data remained challenging. Here, we report SSFinder, a python script to perform high throughput detection of specific target sites in large nucleotide datasets. The SSFinder is a user-friendly tool, compatible with Windows, Mac OS, and Linux operating systems, and freely available online.

High-throughput Detection of Protein Kinase Activities in Cell Lysate Based on the Aggregation of Gold Nanoparticles with Peptides

2009 ◽  
Author(s):  
Yoshiki Katayama ◽  
Hirotaro Kitazaki ◽  
Jeong-Hun Kang ◽  
Xiaoming Han ◽  
Takeshi Mori ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Protein Kinase ◽  
High Throughput ◽  
Cell Lysate ◽  
High Throughput Detection

scholarly journals Fluorescent Probes for Live Cell Thiol Detection

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3575
Author(s):  
Shenggang Wang ◽  
Yue Huang ◽  
Xiangming Guan
Keyword(s):  
Fluorescent Probes ◽  
Biological System ◽  
High Sensitivity ◽  
Intracellular Distribution ◽  
Live Cell ◽  
Live Cells ◽  
High Demand ◽  
Non Invasive

Thiols play vital and irreplaceable roles in the biological system. Abnormality of thiol levels has been linked with various diseases and biological disorders. Thiols are known to distribute unevenly and change dynamically in the biological system. Methods that can determine thiols’ concentration and distribution in live cells are in high demand. In the last two decades, fluorescent probes have emerged as a powerful tool for achieving that goal for the simplicity, high sensitivity, and capability of visualizing the analytes in live cells in a non-invasive way. They also enable the determination of intracellular distribution and dynamitic movement of thiols in the intact native environments. This review focuses on some of the major strategies/mechanisms being used for detecting GSH, Cys/Hcy, and other thiols in live cells via fluorescent probes, and how they are applied at the cellular and subcellular levels. The sensing mechanisms (for GSH and Cys/Hcy) and bio-applications of the probes are illustrated followed by a summary of probes for selectively detecting cellular and subcellular thiols.

High-throughput determination of flavanone-O-glycosides in citrus beverages by paper spray tandem mass spectrometry

2021 ◽  
pp. 130060
Author(s):  
Fabio Mazzotti ◽  
Lucia Bartella ◽  
Ines Rosita Talarico ◽  
Anna Napoli ◽  
Leonardo Di Donna
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
High Throughput ◽  
Tandem Mass ◽  
Paper Spray

High-Throughput Detection of an Alkaloidal Plant Metabolome in Plant Extracts Using LC-ESI-QTOF-MS

2021 ◽  
Author(s):  
Bibi Zareena ◽  
Adeeba Khadim ◽  
Syed Usama Y. Jeelani ◽  
Saddam Hussain ◽  
Arslan Ali ◽  
...  
Keyword(s):  
High Throughput ◽  
Plant Extracts ◽  
High Throughput Detection

Determination of Depleted Uranium in Environmental Bio-monitor Samples and Soil from Target sites in Western Balkan Region

2008 ◽  
Author(s):  
Sarata K. Sahoo ◽  
Hiroko Enomoto ◽  
Shinji Tokonami ◽  
Tetsuo Ishikawa ◽  
Predrag Ujić ◽  
...  
Keyword(s):  
Depleted Uranium ◽  
Target Sites ◽  
Western Balkan Region ◽  
Balkan Region
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