scholarly journals Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain

2019 ◽  
Vol 117 (2) ◽  
pp. 543-555 ◽  
Author(s):  
Joseph R. Brady ◽  
Charles A. Whittaker ◽  
Melody C. Tan ◽  
D. Lee Kristensen ◽  
Duanduan Ma ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Scale Analysis ◽  
Comparative Genome ◽  
Genome Scale ◽  
Base Strain ◽  
Selection Of

scholarly journals Positive Selection of Novel Peroxisome Biogenesis-Defective Mutants of the Yeast Pichia pastoris

Genetics ◽  
1999 ◽  
Vol 151 (4) ◽  
pp. 1379-1391
Author(s):  
Monique A Johnson ◽  
Hans R Waterham ◽  
Galyna P Ksheminska ◽  
Liubov R Fayura ◽  
Joan Lin Cereghino ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Allyl Alcohol ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeast ◽  
Direct Selection ◽  
Toxic Exposure ◽  
Peroxisome Biogenesis ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Selection Of

Abstract We have developed two novel schemes for the direct selection of peroxisome-biogenesis-defective (pex) mutants of the methylotrophic yeast Pichia pastoris. Both schemes take advantage of our observation that methanol-induced pex mutants contain little or no alcohol oxidase (AOX) activity. AOX is a peroxisomal matrix enzyme that catalyzes the first step in the methanol-utilization pathway. One scheme utilizes allyl alcohol, a compound that is not toxic to cells but is oxidized by AOX to acrolein, a compound that is toxic. Exposure of mutagenized populations of AOX-induced cells to allyl alcohol selectively kills AOX-containing cells. However, pex mutants without AOX are able to grow. The second scheme utilizes a P. pastoris strain that is defective in formaldehyde dehydrogenase (FLD), a methanol pathway enzyme required to metabolize formaldehyde, the product of AOX. AOX-induced cells of fld1 strains are sensitive to methanol because of the accumulation of formaldehyde. However, fld1 pex mutants, with little active AOX, do not efficiently oxidize methanol to formaldehyde and therefore are not sensitive to methanol. Using these selections, new pex mutant alleles in previously identified PEX genes have been isolated along with mutants in three previously unidentified PEX groups.

Selection of Pichia pastoris strains expressing recombinant immunoglobulin G by cell surface labeling

2010 ◽  
Vol 358 (1-2) ◽  
pp. 66-74 ◽  
Author(s):  
Song Lin ◽  
Zheng Shen ◽  
Dongxing Zha ◽  
Nathan Sharkey ◽  
Bianka Prinz ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Cell Surface ◽  
Immunoglobulin G ◽  
Selection Of

Integrated Genome-Scale Analysis and Northern Blot Detection of Retrotransposon siRNAs Across Plant Species

2020 ◽  
pp. 387-411
Author(s):  
Marcel Böhrer ◽  
Bart Rymen ◽  
Christophe Himber ◽  
Aude Gerbaud ◽  
David Pflieger ◽  
...  
Keyword(s):  
Plant Species ◽  
Northern Blot ◽  
Scale Analysis ◽  
Genome Scale

scholarly journals Genome-scale analysis of evolutionary rate and selection in a fast-expanding Spanish cluster of HIV-1 subtype F1

2018 ◽  
Vol 66 ◽  
pp. 43-47 ◽  
Author(s):  
Juan Á. Patiño-Galindo ◽  
Francisco Domínguez ◽  
María T. Cuevas ◽  
Elena Delgado ◽  
Mónica Sánchez ◽  
...  
Keyword(s):  
Evolutionary Rate ◽  
Scale Analysis ◽  
Genome Scale ◽  
Hiv 1

Identification and validation of tumor environment phenotypes in lung adenocarcinoma by integrative genome-scale analysis

2020 ◽  
Vol 69 (7) ◽  
pp. 1293-1305
Author(s):  
Guoshu Bi ◽  
Zhencong Chen ◽  
Xiaodong Yang ◽  
Jiaqi Liang ◽  
Zhengyang Hu ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Scale Analysis ◽  
Tumor Environment ◽  
Genome Scale

scholarly journals New Method for the Selection of Multicopy Transformants of Pichia pastoris, Using 3-Amino-1,2,4 Triazol

BioTechniques ◽  
2000 ◽  
Vol 29 (5) ◽  
pp. 1094-1099 ◽  
Author(s):  
Javier Menéndez ◽  
Bianca García ◽  
Yurima Hidalgo
Keyword(s):  
Pichia Pastoris ◽  
New Method ◽  
Selection Of
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