RNA‐dependent chaperone (chaperna) as an engineered pro‐region for the folding of recombinant microbial transglutaminase

2019 ◽  
Vol 116 (3) ◽  
pp. 490-502 ◽  
Author(s):  
Jinhee Lee ◽  
Ahyun Son ◽  
Paul Kim ◽  
Soon Bin Kwon ◽  
Ji Eun Yu ◽  
...  
Keyword(s):  
Microbial Transglutaminase ◽  
Pro Region ◽  
Recombinant Microbial Transglutaminase

Gelation of Food Protein Induced by Recombinant Microbial Transglutaminase

2003 ◽  
Vol 68 (1) ◽  
pp. 48-51 ◽  
Author(s):  
K. Yokoyama ◽  
T. Ohtsuka ◽  
C. Kuraishi ◽  
K. Ono ◽  
Y. Kita ◽  
...  
Keyword(s):  
Microbial Transglutaminase ◽  
Food Protein ◽  
Recombinant Microbial Transglutaminase

scholarly journals The pro region of human intestinal lactase-phlorizin hydrolase.

1994 ◽  
Vol 269 (43) ◽  
pp. 26933-26943
Author(s):  
H Y Naim ◽  
R Jacob ◽  
H Naim ◽  
J F Sambrook ◽  
M J Gething
Keyword(s):  
Pro Region

scholarly journals Characterization of Gelatin Hydrogels Cross-Linked with Microbial Transglutaminase as Engineered Skeletal Muscle Substrates

2021 ◽  
Vol 8 (1) ◽  
pp. 6
Author(s):  
Divya Gupta ◽  
Jeffrey W. Santoso ◽  
Megan L. McCain
Keyword(s):  
Skeletal Muscle ◽  
Elastic Modulus ◽  
Ambient Air ◽  
In Vitro Models ◽  
Microbial Transglutaminase ◽  
Conventional Culture ◽  
Muscle Tissues ◽  
Physical Features ◽  
Phosphate Buffered Saline

Engineered in vitro models of skeletal muscle are essential for efficiently screening drug safety and efficacy. However, conventional culture substrates poorly replicate physical features of native muscle and do not support long-term culture, which limits tissue maturity. Micromolded gelatin hydrogels cross-linked with microbial transglutaminase (gelatin-MTG hydrogels) have previously been shown to induce C21C2 myotube alignment and improve culture longevity. However, several properties of gelatin-MTG hydrogels have not been systematically characterized, such as changes in elastic modulus during incubation in culture-like conditions and their ability to support sarcomere maturation. In this study, various gelatin-MTG hydrogels were fabricated and incubated in ambient or culture-like conditions. Elastic modulus, mass, and transmittance were measured over a one- or two-week period. Compared to hydrogels in phosphate buffered saline (PBS) or ambient air, hydrogels in Dulbecco’s Modified Eagle Medium (DMEM) and 5% CO2 demonstrated the most stable elastic modulus. A subset of gelatin-MTG hydrogels was micromolded and seeded with C2C12 or primary chick myoblasts, which aligned and fused into multinucleated myotubes with relatively mature sarcomeres. These data are important for fabricating gelatin-MTG hydrogels with predictable and stable mechanical properties and highlight their advantages as culture substrates for engineering relatively mature and stable muscle tissues.

scholarly journals Secretion of functional papain precursor from insect cells. Requirement for N-glycosylation of the pro-region.

1990 ◽  
Vol 265 (27) ◽  
pp. 16661-16666 ◽  
Author(s):  
T Vernet ◽  
D C Tessier ◽  
C Richardson ◽  
F Laliberté ◽  
H E Khouri ◽  
...  
Keyword(s):  
Insect Cells ◽  
Pro Region
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