Enhanced cellulase recovery without β-glucosidase supplementation for cellulosic ethanol production using an engineered strain and surfactant

2016 ◽  
Vol 114 (3) ◽  
pp. 543-551 ◽  
Author(s):  
Renliang Huang ◽  
Hong Guo ◽  
Rongxin Su ◽  
Wei Qi ◽  
Zhimin He
Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 173
Author(s):  
Elena Domínguez ◽  
Pablo G. del Río ◽  
Aloia Romaní ◽  
Gil Garrote ◽  
Lucília Domingues

In order to exploit a fast-growing Paulownia hardwood as an energy crop, a xylose-enriched hydrolysate was obtained in this work to increase the ethanol concentration using the hemicellulosic fraction, besides the already widely studied cellulosic fraction. For that, Paulownia elongata x fortunei was submitted to autohydrolysis treatment (210 °C or S0 of 4.08) for the xylan solubilization, mainly as xylooligosaccharides. Afterwards, sequential stages of acid hydrolysis, concentration, and detoxification were evaluated to obtain fermentable sugars. Thus, detoxified and non-detoxified hydrolysates (diluted or not) were fermented for ethanol production using a natural xylose-consuming yeast, Scheffersomyces stipitis CECT 1922, and an industrial Saccharomyces cerevisiae MEC1133 strain, metabolic engineered strain with the xylose reductase/xylitol dehydrogenase pathway. Results from fermentation assays showed that the engineered S. cerevisiae strain produced up to 14.2 g/L of ethanol (corresponding to 0.33 g/g of ethanol yield) using the non-detoxified hydrolysate. Nevertheless, the yeast S. stipitis reached similar values of ethanol, but only in the detoxified hydrolysate. Hence, the fermentation data prove the suitability and robustness of the engineered strain to ferment non-detoxified liquor, and the appropriateness of detoxification of liquor for the use of less robust yeast. In addition, the success of hemicellulose-to-ethanol production obtained in this work shows the Paulownia biomass as a suitable renewable source for ethanol production following a suitable fractionation process within a biorefinery approach.


Author(s):  
Carlos Eduardo de Araújo Padilha ◽  
Cleitiane da Costa Nogueira ◽  
Bárbara Ribeiro Alves Alencar ◽  
Íthalo Barbosa Silva de Abreu ◽  
Emmanuel Damilano Dutra ◽  
...  

2012 ◽  
Vol 51 (6-7) ◽  
pp. 366-372 ◽  
Author(s):  
Seung-Ho Baek ◽  
Sujin Kim ◽  
Kyusung Lee ◽  
Jung-Kul Lee ◽  
Ji-Sook Hahn

2011 ◽  
Vol 02 (10) ◽  
pp. 1303-1309 ◽  
Author(s):  
Fabiano A. Gonçalves ◽  
Eliana J. Sanjinez-Argandoña ◽  
Gustavo G. Fonseca

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0134964 ◽  
Author(s):  
Viviane Guzzo de Carli Poelking ◽  
Andrea Giordano ◽  
Maria Esther Ricci-Silva ◽  
Thomas Christopher Rhys Williams ◽  
Diego Alves Peçanha ◽  
...  

Author(s):  
Craig Evans

Alico Energy, LLC, a subsidiary of Alico, Inc., has been awarded a grant from the State of Florida under the Florida Energy Act and a grant and loan guarantee from the U.S. Department of Energy to construct one of the nation’s first cellulosic ethanol facilities in South Florida. The Alico Energy biorefinery will be capable of co-producing ethanol and electricity from any carbon-based feedstock or waste, including citrus peel, and trimmings from citrus trees. The initial plant will produce only ethanol. The capacity to produce electricity will be added at a later date. Paper published with permission.


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