Fabrication of highly porous scaffolds for tissue engineering based on star-shaped functional poly(ε-caprolactone)

2010 ◽  
Vol 108 (3) ◽  
pp. 694-703 ◽  
Author(s):  
Stefan Theiler ◽  
Petra Mela ◽  
Stefanos E. Diamantouros ◽  
Stefan Jockenhoevel ◽  
Helmut Keul ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Porous Scaffolds ◽  
Highly Porous

scholarly journals Highly porous scaffolds of PEDOT:PSS for bone tissue engineering

2017 ◽  
Vol 62 ◽  
pp. 91-101 ◽  
Author(s):  
Anne Géraldine Guex ◽  
Jennifer L. Puetzer ◽  
Astrid Armgarth ◽  
Elena Littmann ◽  
Eleni Stavrinidou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffolds ◽  
Highly Porous

scholarly journals Synthesis and Investigation into Apatite-forming Ability of Hydroxyapatite/Chitosan-based Scaffold

2019 ◽  
Vol 35 (3) ◽  
Author(s):  
Tran Thanh Hoai ◽  
Nguyen Kim Nga
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Inorganic Material ◽  
Porous Scaffolds ◽  
Mineral Layer ◽  
Chitosan Scaffold ◽  
Apatite Mineral ◽  
Highly Porous

In this study, porous scaffolds were fabricated using inorganic material-hydroxyapatite and chitosan for bone-tissue engineering. The combination of hydroxyapatite and chitosan may result in increasing biocompatibility of the scaffolds. The scaffolds were prepared by solvent casting and paticulate leaching method. Bioactivity of the scaffolds was evaluated through in vitro experiments by soaking scaffold samples in simulated body fluid (SBF). The scaffolds obtained were highly porous and interconnected with a mean pore size of around 200µm and porosity about 79 %. The apatite-mineral layer was produced on the HAp/chitosan after 10 days of soaking in SBF, however, it was not observed on the chitosan scaffold after 10 days soaking. The results revealed that the HAp/chitosan scaffold showed better bioactivity than the chitosan scaffold. Keywords Scaffold, Chitosan, Apatite, SBF. In this study, porous scaffolds were fabricated using inorganic material-hydroxyapatite and chitosan for bone-tissue engineering. The combination of hydroxyapatite and chitosan may result in increasing biocompatibility of the scaffolds. The scaffolds were prepared by solvent casting and paticulate leaching method. Bioactivity of the scaffolds was evaluated through in vitro experiments by soaking scaffold samples in simulated body fluid (SBF). The scaffolds obtained were highly porous and interconnected with a mean pore size of around 200µm and porosity about 79 %. The apatite-mineral layer was produced on the HAp/chitosan after 10 days of soaking in SBF, however, it was not observed on the chitosan scaffold after 10 days soaking. The results revealed that the HAp/chitosan scaffold showed better bioactivity than the chitosan scaffold. Keywords: Scaffold, Chitosan, Apatite, SBF.   In this study, porous scaffolds were fabricated using inorganic material-hydroxyapatite and chitosan for bone-tissue engineering. The combination of hydroxyapatite and chitosan may result in increasing biocompatibility of the scaffolds. The scaffolds were prepared by solvent casting and paticulate leaching method. Bioactivity of the scaffolds was evaluated through in vitro experiments by soaking scaffold samples in simulated body fluid (SBF). The scaffolds obtained were highly porous and interconnected with a mean pore size of around 200µm and porosity about 79 %. The apatite-mineral layer was produced on the HAp/chitosan after 10 days of soaking in SBF, however, it was not observed on the chitosan scaffold after 10 days soaking. The results revealed that the HAp/chitosan scaffold showed better bioactivity than the chitosan scaffold. Keywords: Scaffold, Chitosan, Apatite, SBF. References [1] M.P. Bostrom, D.A. Seigerman, The clinical use of allografts, demineralized bone matrices, synthetic bone graft substitutes and osteoinductive growth factors: a survey study, Hss. Journal 1 (2005) 9-18. https://doi.org/10. 1007/s11420-005-0111-5.[2] T.T. Hoai, N.K Nga, L.T. Giang, T.Q. Huy, P.N.M. Tuan, B.T.T. Binh, Hydrothermal Synthesis of Hydroxyapatite Nanorods for Rapid Formation of Bone-Like Mineralization, J. Electron. Mater. 46 (2017) 5064-5072. https:// doi.org/10.1007/s11664-017-5509-6.[3] M. Rinaudo, Chitin and chitosan: properties and applications, Prog. Polym. Sci. 31 (2006) 603-632. https://doi.org/10.1016/j.progpolymsci.2006. 06.001.[4] N.K. Nga, H.D. Chinh, P.T.T Hong, T.Q. Huy, Facile chitosan films for high performance removal of reactive blue 19 dye from aqueous solution, J. Polym. Environ. 25 (2007) 146-155. https://doi.org/10.1007/s10924-016-0792-5.[5] M.N.V Ravi Kumar, R.A.A Muzzarelli, H. Sashiwa, A.J. Domb, Chitosan chemistry and pharmaceutical perspectives, Chem. Rev. 104 (2004) 6017-6084. https://doi.org/10.1021/cr03 0441b.[6] J.M. Karp, M.S. Shoichet, J.E. Davies, Bone formation on two‐dimensional poly (DL‐lactide‐co‐glycolide)(PLGA) films and three‐dimensional PLGA tissue engineering scaffolds in vitro, J. Biomed. Mater. Res. A 64 (2003) 388-396. https://doi.org/10.1002/jbm.a.10420.[7] J.F. Mano, R.L. Reis, Osteochondral defects: present situation and tissue engineering approaches, J. Tissue. Eng. Regen. Med. 1 (2007) 261-273. https://doi.org/10.1002/term.37. [8] A.G. Mikos, J.S. Temenoff, Formation of highly porous biodegradable scaffolds for tissue engineering, Electron. J. Biotechn. 3 (2000) 23-24. http://dx.doi.org/10.4067/S0717-3458200000 0200003.[9] W.W. Thein-Han, R.D.K Misra, Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering, Acta Biomater. 5 (2009) 1182–1197. https://doi.org/ 10.1016/j.actbio.2008.11.025.[10] Y. Zhang, J.R. Venugopal, A.E. Turki, S. Ramakrishna, B. Su, C.T. Lim, Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering, Biomaterials 29 (2008) 4314–4322. https://doi.org/10.1016/j.biomaterials.2008.07.038.[11] B.X. Vương, Tổng hợp và đặc trưng vật liệu composite hydroxyapatite/chitosan ứng dụng trong kỹ thuật y sinh.,Tạp chí Khoa học ĐHQGHN: Khoa học Tự nhiên và Công nghệ Tập 34 (2018) 9-15. https://doi.org/10.25073/ 2588-1140/vnunst.4689.[12] N.K. Nga, T.T. Hoai, P.H. Viet, Biomimetic scaffolds based on hydroxyapatite nanorod/poly (D, L) lactic acid with their corresponding apatite-forming capability and biocompatibility for bone-tissue engineering, Colloids Surf. B Biointerf. 128 (2015) 506-514. https://doi.org/10. 1016/j.colsurfb.2015.03.001.[13] N.K. Nga, L.T. Giang, T.Q. Huy, C. Migliaresi, Surfactant-assisted size control of hydroxyapatite nanorods for bone tissue engineering, Colloids Surf. B: Biointerf. 116 (2014) 666-673. https://doi.org/10.1016/j.colsurfb.2013.11.001.[14] C.R. Kothapalli, M.T. Shaw, M. Wei, Biodegradable HA-PLA 3-D porous scaffolds: effect of nano-sized filler content on scaffold properties, Acta Biomater. 1 (2005) 653-662. https://doi.org/10.1016/j.actbio.2005.06.005.[15] T. Kokubo, H. Takadama, How useful is SBF in predicting in vivo bone bioactivity?, Biomaterials 27 (2006) 2907-2915. https://doi.org/10.1016/j. biomaterials.2006.01.017[16] T.T. Hoai, N.K. Nga, Effect of pore architecture on osteoblast adhesion and proliferation on hydroxyapatite/poly (D, L) lactic acid-based bone scaffolds, J. Iran. Chem. Soc. 15 (2018) 1663-1671. https://doi.org/10.1007/s13738-018-1365-4.        

Effect of Different Concentration of Cellulose Nanocrystals Comprising Hydroxyethyl Cellulose / Poly(Vinyl Alcohol) as a Bone Tissue Engineering Scaffold

2020 ◽  
Vol 981 ◽  
pp. 285-290
Author(s):  
Nor Sarahtul Nadirah Hairol Nizan ◽  
Farah Hanani Zulkifli ◽  
Hazrulrizawati Abd Hamid ◽  
Muhammad Hafiz Mazwir
Keyword(s):  
Tissue Engineering ◽  
Thermal Properties ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Vinyl Alcohol ◽  
Hydroxyethyl Cellulose ◽  
Poly Vinyl Alcohol ◽  
Porous Scaffolds ◽  
Cytotoxicity Studies ◽  
Highly Porous

In this study, biodegradable scaffolds based on hydroxyethyl cellulose (HEC) (5 wt%) and poly (vinyl alcohol) (PVA) (15 wt%) with different percentages of celullose nanocrystal (CNC) (1 and 7 wt%) were fabricated by lyophilization method to get highly porous scaffolds. These scaffolds were made water insoluble by cross-linking via heat treatment. The morphology and thermal properties of HEC/PVA/CNCs scaffolds were characterized by using Scanning Electron Microscope (SEM) and Thermogravimetric Analysis (TGA). The morphological study showed that both prepared scaffold have highly porous structures with good pore interconnected structure. It was observed that thermal properties of scaffolds increased significantly as the concentration of CNCs increased. Cytotoxicity studies on scaffolds were carried out by utilizing human fetal osteoblast (hFOB) cells using DAPI nuclear stain and then confirmed using SEM. hFOB cells were able to attach and spread on all scaffolds. Incorporated CNCs as reinforcing nanofiller on scaffolds promising a superior functionality in bone tissue engineering.

Development of highly porous scaffolds based on bioactive silicates for dental tissue engineering

2014 ◽  
Vol 49 ◽  
pp. 399-404 ◽  
Author(s):  
O.M. Goudouri ◽  
E. Theodosoglou ◽  
E. Kontonasaki ◽  
J. Will ◽  
K. Chrissafis ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Porous Scaffolds ◽  
Dental Tissue ◽  
Dental Tissue Engineering ◽  
Highly Porous

scholarly journals Porous scaffolds with the structure of an interpenetrating polymer network made by gelatin methacrylated nanoparticle-stabilized high internal phase emulsion polymerization targeted for tissue engineering

RSC Advances ◽  
2021 ◽  
Vol 11 (37) ◽  
pp. 22544-22555
Author(s):  
Atefeh Safaei-Yaraziz ◽  
Shiva Akbari-Birgani ◽  
Nasser Nikfarjam
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Growth ◽  
Polymer Network ◽  
Synthetic Polymers ◽  
Tissue Scaffolds ◽  
Interpenetrating Polymer Network ◽  
Porous Scaffolds ◽  
Promising Strategy ◽  
Internal Phase

The interlacing of biopolymers and synthetic polymers is a promising strategy to fabricate hydrogel-based tissue scaffolds to biomimic a natural extracellular matrix for cell growth.

scholarly journals Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1319
Author(s):  
Muhammad Umar Aslam Khan ◽  
Wafa Shamsan Al-Arjan ◽  
Mona Saad Binkadem ◽  
Hassan Mehboob ◽  
Adnan Haider ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Guar Gum ◽  
Porous Scaffolds ◽  
Vitro Assay ◽  
Vitro Analysis ◽  
Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

scholarly journals Macroporous chitosan/methoxypoly(ethylene glycol) based cryosponges with unique morphology for tissue engineering applications

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pradeep Kumar ◽  
Viness Pillay ◽  
Yahya E. Choonara
Keyword(s):  
Tissue Engineering ◽  
Polymer Network ◽  
Three Dimensional ◽  
Hysteresis Loops ◽  
Interpenetrating Polymer Network ◽  
Morphological Data ◽  
Porous Scaffolds ◽  
Morphological Variations ◽  
Molecular Stability ◽  
The Matrix

AbstractThree-dimensional porous scaffolds are widely employed in tissue engineering and regenerative medicine for their ability to carry bioactives and cells; and for their platform properties to allow for bridging-the-gap within an injured tissue. This study describes the effect of various methoxypolyethylene glycol (mPEG) derivatives (mPEG (-OCH3 functionality), mPEG-aldehyde (mPEG-CHO) and mPEG-acetic acid (mPEG-COOH)) on the morphology and physical properties of chemically crosslinked, semi-interpenetrating polymer network (IPN), chitosan (CHT)/mPEG blend cryosponges. Physicochemical and molecular characterization revealed that the –CHO and –COOH functional groups in mPEG derivatives interacted with the –NH2 functionality of the chitosan chain. The distinguishing feature of the cryosponges was their unique morphological features such as fringe thread-, pebble-, curved quartz crystal-, crystal flower-; and canyon-like structures. The morphological data was well corroborated by the image processing data and physisorption curves corresponding to Type II isotherm with open hysteresis loops. Functionalization of mPEG had no evident influence on the macro-mechanical properties of the cryosponges but increased the matrix strength as determined by the rheomechanical analyses. The cryosponges were able to deliver bioactives (dexamethasone and curcumin) over 10 days, showed varied matrix degradation profiles, and supported neuronal cells on the matrix surface. In addition, in silico simulations confirmed the compatibility and molecular stability of the CHT/mPEG blend compositions. In conclusion, the study confirmed that significant morphological variations may be induced by minimal functionalization and crosslinking of biomaterials.

Characterisation of ‘wet’ polymer surfaces for tissue engineering applications: Are flat surfaces a suitable model for complex structures?

e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Laleh Safinia ◽  
Jonny J. Blaker ◽  
Véronique Maquet ◽  
Aldo R. Boccaccini ◽  
Athanassios Mantalaris ◽  
...  
Keyword(s):  
Thin Films ◽  
Tissue Engineering ◽  
Pore Wall ◽  
Polymer Surfaces ◽  
Suitable Model ◽  
Tissue Engineering Scaffolds ◽  
3D Structures ◽  
Flat Surfaces ◽  
Point Test ◽  
Highly Porous

AbstractTissue engineering scaffolds are 3D constructs that simulate the growth environment in vivo. The present work aims to address the question of whether thin films, i.e., flat surfaces, are a suitable model for more complex 3D structures? With this in mind a complete study of the morphology and surface chemistry of poly(D,Llactide) (PDLLA) substrates, fabricated into two different structures, is presented. The polymer structures studied include a 3D, porous, foam-like scaffold prepared by the thermally induced phase separation (TIPS) method and flat polymer thin films made by solvent casting. Based on the maximum bubble point test, a new method to assess the wettability of wet pore wall surfaces inside highly porous 3D structures was developed and tested. The maximum pore diameter determined using the maximum bubble point test for the total wetting liquids was confirmed through image analysis of scanning electron micrographs. The method allows the determination of the contact angle between the wet pore wall and a contacting liquid. The captive bubble method was employed to characterise the wettability of flat polymer films in contact with water. Both structures were further characterised using zeta- (ζ-) potential measurements to assess the surface chemistry of the polymer. The results demonstrate that PDLLA contains acidic functional groups and is hydrophobic. In order to evaluate the sensitivity of the test methods, the polymer surfaces were modified by protein adsorption using fibronectin and collagen. ζ-Potential and wettability measurements show that proteins indeed adsorb on virgin PDLLA surfaces. Protein adsorption causes the wettability of the PDLLA for water to improve. Our results strongly indicate that flat surfaces are not a suitable model for surfaces in complex 3D structures such as highly porous tissue engineering scaffolds. Such scaffolds must be characterised as a 3D system.

scholarly journals Productionof poly(L-CO-D,L LacticAcid) porous fibers by electrospinning

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Nayara Maysa da Silva Carvalho ◽  
Bárbara E. Ciocca ◽  
Rubens Maciel Filho ◽  
Marcele Fonseca Passos ◽  
Maria Regina Wolf Maciel ◽  
...  
Keyword(s):  
Pore Formation ◽  
Scientific Community ◽  
Low Dielectric Constant ◽  
Porous Scaffolds ◽  
Low Density ◽  
Fiber Morphology ◽  
Low Dielectric ◽  
Mean Diameter ◽  
Porous Fibers ◽  
Highly Porous

The production of porous scaffolds has been widely investigated by the scientific community due to its suitability for tissue engineering. Among techniques that allow the fabrication of porous materials, electrospinning is appealing for being robust and versatile. This research investigated the pore formation in poly (L-co-D,L lactic acid) fibers obtained by conventional electrospinning and the influence of chloroform as a single solvent on fiber morphology. Random and highly porous fibers with a mean diameter of 2.373 ± 0.564 µm were collected. Chloroform affects the fiber morphology, mainly for its fast evaporation and low density of charges. The solvent on the surface evaporates quickly, and the low stretch of the jet does not help the polymer to reorganize over the length of the fiber, forming pores. In conclusion, the low dielectric constant and boiling point of chloroform induce pores formation along the PLDLA fibers.  

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