Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions

2003 ◽  
Vol 82 (6) ◽  
pp. 684-690 ◽  
Author(s):  
Enhong Cao ◽  
Yahuei Chen ◽  
Zhanfeng Cui ◽  
Peter R. Foster
Keyword(s):  
Aqueous Solutions ◽  
Freezing And Thawing ◽  
Freezing And Thawing Rates ◽  
Denaturation Of Proteins

Comparative cryopreservation of avian spermatozoa: Effects of freezing and thawing rates on turkey and sandhill crane sperm cryosurvival

2012 ◽  
Vol 131 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
Juan M. Blanco ◽  
Julie A. Long ◽  
George Gee ◽  
David E. Wildt ◽  
Ann M. Donoghue
Keyword(s):  
Freezing And Thawing ◽  
Sandhill Crane ◽  
Freezing And Thawing Rates

scholarly journals THE EFFECT OF STORAGE CONDITIONS ON THE PROPERTIES OF AQUEOUS SOLUTIONS OF CARBOXYMETHYLATED PLANT MATERIALS

2018 ◽  
pp. 309-317 ◽  
Author(s):  
Елена (Yelena) Владимировна (Vladimirovna) Калюта (Kalyuta) ◽  
Вадим (Vadim) Иванович (Ivanovich) Маркин (Markin) ◽  
Михаил (Mikhail) Ильич (Il'ich) Мальцев (Mal'tsev)
Keyword(s):  
Plant Growth ◽  
Aqueous Solutions ◽  
Agricultural Production ◽  
Raw Materials ◽  
Storage Conditions ◽  
Freezing And Thawing ◽  
Plant Materials ◽  
Plant Raw Materials ◽  
Urgent Task ◽  
Vegetable Raw Materials

The development of new plant growth regulators based on products of chemical processing of plant raw materials is currently an urgent task for increasing yields in agricultural production. On the basis of carboxymethylated plant materials, "Eco-Stim" plant growth regulator has been developed. The purpose of this work is to study the effect of the storage conditions of solutions of carboxymethylated plant materials in "Eco-Stim" as part of the preparation on their rheological and growth-regulating properties. The rheological properties of the aqueous systems of carboxymethylated pine, sunflower, and oat chaff have been studied at concentrations of 0.2–15%. It is established that the change in viscosity as a result of the shear rate of the systems studied is more complex in comparison with aqueous solutions of Na-CMC. Storage of solutions of carboxymethylated vegetable raw materials for 20 months, as well as the processes of freezing and thawing for 6–9 weeks have practically no effect on the viscosity and growth regulating properties of carboxymethylated vegetable raw materials. The studied systems make it possible to increase the germination of wheat seeds Omskaya-36 to 80–87% and to increase the length of the root by 4–10 times and the stem by 1.5–2 times compared to the control.

scholarly journals Effect of freezing and thawing rates on sperm motility in Bocachico Prochilodus magdalenae (Pisces, Characiformes)

2013 ◽  
pp. 3295-3303 ◽  
Author(s):  
José G. Martínez ◽  
Sandra Pardo C
Keyword(s):  
Slow Freezing ◽  
Rapid Freezing ◽  
Sperm Viability ◽  
Sperm Cryopreservation ◽  
Freezing And Thawing ◽  
Straight Line ◽  
Independent Effects ◽  
Thawing Rate ◽  
Main Effects ◽  
Freezing And Thawing Rates

ABSTRACTObjective. To determine the freezing and thawing rates necessary to maintain sperm viability during cryopreservation of Bocachico semen. Materials and methods. Four interactional treatments were implemented between two freezing (rapid and slow) and two thawing (rapid and slow) curves, in a 2x2 factorial as follows: rapid freezing-rapid thawing, rapid freezing-slow thawing, slow freezing-rapid thawing, and slow freezing-slow thawing. After thawing by Sperm Class Analyzer (SCA) curvilinear velocity (VCL) and straight-line (VSL) (μm sec-1) were analyzed; total, rapid, medium, and slow motility, were compared among treatments. Results. The rapid freezing-slow thawing treatment was lethal for all variables of velocity and motility, causing a significant (p<0.01) post-thaw inmotility of 100%. The slow freezing-rapid thawing interaction had a significantly higher effect than the other treatments (p<0.05), particularly on variables such as rapid motility (10.1 ± 1.1%), medium motility (30.16 ± 4.1%), and curvilinear velocity (51.5 ± 4.75 μm sec.-1) also decreased the percentage of sperm with slow motility (41.7 ± 4.45%). Independently of the applied thawing rate, the freezing rate generated the main significant effect on total motility. Conclusions. It is possible to conclude that the interaction effect between freezing and thawing rates is nil (except for slow motility) during cryopreservation process. However, the independent effects of these factors (main effects) on remaining motility variables are positively significant and decisive to the maintenance of these features, especially the freeze factor (when it is slow). This becomes the first successful report of sperm cryopreservation from Bocachico Prochilodus magdalenae in the world and may be used in conservation programs for this endangered species.

GIANT PANDA SPERM TOLERATE CRYOPRESERVATION AT RAPID FREEZING AND THAWING RATES

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 107-108 ◽  
Author(s):  
Copper Aitken-Palmer ◽  
Rong Hou ◽  
David Wildt ◽  
Mary Ann Ottinger ◽  
Rebecca Spindler ◽  
...  
Keyword(s):  
Giant Panda ◽  
Rapid Freezing ◽  
Freezing And Thawing ◽  
Freezing And Thawing Rates

Rates and intensity of freeze–thaw cycles affect nitrous oxide and carbon dioxide emissions from agricultural soils

2019 ◽  
Vol 99 (4) ◽  
pp. 472-484 ◽  
Author(s):  
David E. Pelster ◽  
Martin H. Chantigny ◽  
Philippe Rochette ◽  
Normand Bertrand ◽  
Denis A. Angers ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Nitrous Oxide ◽  
Carbon Dioxide Emissions ◽  
Slow Freezing ◽  
Control Treatment ◽  
Rapid Freezing ◽  
Silty Clay ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Freezing And Thawing Rates

In cool temperate regions, large emissions of nitrous oxide (N2O), an important greenhouse and ozone-depleting gas, have been observed during freeze–thaw (FT) cycles. However, it is unclear how freezing and thawing rates, freezing intensity, and freezing duration influence N2O emissions. We used a laboratory incubation to measure N2O emissions from two soils (sandy loam, silty clay) undergoing a single FT cycle of various freezing and thawing rates [rapid (0.5 °C h−1) vs. slow (0.017 °C h−1)], freezing intensity (−1 vs. −3 °C), and freezing duration (24 vs. 48 freezing degree-days). In general, soil carbon dioxide fluxes during freezing were highest when soils were frozen slowly at −1 °C, whereas fluxes after thawing were highest from the soils frozen and thawed rapidly at −3 °C. Soil N2O emissions during both the freezing and thawing periods were greatest in the soils exposed to rapid freezing to −3 °C, intermediate under rapid freezing to −1 °C and slow freezing to −3 °C, and smallest under slow freezing to −1 °C and the control treatment (constant +1 °C). The similar N2O emissions between the unfrozen control and the slowly frozen −1 °C treatment was unexpected as previous field studies with similar freezing rates and temperatures still experienced high N2O emissions during thaw. This suggests that the physical disruptions caused by freezing and thawing of the surface soil are not the primary driver of FT-induced N2O emissions under field conditions.

FREEZING AND THAWING RATES OF LAMB CHOPS: EFFECTS ON PALATABILITY AND RELATED CHARACTERISTICS

1971 ◽  
Vol 36 (4) ◽  
pp. 629-631 ◽  
Author(s):  
MARTHA L. LIND ◽  
DOROTHY L. HARRISON ◽  
DONALD H. KROPF
Keyword(s):  
Freezing And Thawing ◽  
Freezing And Thawing Rates

Effect of varying freezing and thawing rates in experimental cryosurgery

Cryobiology ◽  
1985 ◽  
Vol 22 (2) ◽  
pp. 175-182 ◽  
Author(s):  
Andrew A. Gage ◽  
Katrina Guest ◽  
Mario Montes ◽  
Joseph A. Caruana ◽  
Daniel A. Whalen
Keyword(s):  
Freezing And Thawing ◽  
Freezing And Thawing Rates
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