Nondestructive near-infrared spectroscopic measurement of multiple analytes in undiluted samples of serum-based cell culture media

2001 ◽  
Vol 77 (1) ◽  
pp. 73-82 ◽  
Author(s):  
Martin Rhiel ◽  
Michael B. Cohen ◽  
David W. Murhammer ◽  
Mark A. Arnold
Keyword(s):  
Cell Culture ◽  
Near Infrared ◽  
Culture Media ◽  
Spectroscopic Measurement ◽  
Cell Culture Media ◽  
Infrared Spectroscopic ◽  
Multiple Analytes

Automated On-Line Noninvasive Optical Glucose Monitoring in a Cell Culture System

2002 ◽  
Vol 56 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Byungjo Jung ◽  
Seungjun Lee ◽  
In Hong Yang ◽  
Theresa Good ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Glucose Concentration ◽  
Culture System ◽  
Near Infrared ◽  
Culture Media ◽  
Cell Culture System ◽  
Cell Culture Media ◽  
Line Measurement ◽  
On Line ◽  
Absorbance Spectra

Current techniques for monitoring glucose concentration during cell culture are invasively performed using an off-line measurement system. Even though in situ or ex situ analyte sensors have been tried for on-line measurement, they suffer from difficulties including the inability to keep them sterile, their limited lifetime, and their lack of stability. In this research an alternative optical noninvasive on-line monitoring system based on near-infrared absorbance spectroscopy was developed to measure glucose concentration of cell culture media in a rotary cell culture system. The system included an automatic sampling component controlled by a LabVIEW program and a high-throughput fiber coupling component connected to a Fourier transform near-infrared spectrometer. The effectiveness of the system was investigated via noninvasive on-line measurement of glucose absorbance spectra in the cell culture media during T-cell culture. Glucose absorbance spectra were collected in the spectral range of 2.0 to 2.5 μm. Partial least-squares regression was employed to build a successful multivariate calibration model. The standard error of prediction and mean percent error for glucose were 7.7 mg/dL and 1.0%, respectively. The successful results represent an important step in the development of a noninvasive, closed-loop, system for monitoring analytes in cell culture.

Near-Infrared Spectroscopy for Determination of Glucose, Lactate, and Ammonia in Cell Culture Media

1998 ◽  
Vol 52 (8) ◽  
pp. 1073-1078 ◽  
Author(s):  
Michael J. McShane ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Culture Media ◽  
Clinical Chemistry ◽  
Calibration Model ◽  
Aqueous Mixtures ◽  
Cell Culture Media ◽  
Combining Data

The potential for noninvasive determinations of glucose, lactate, and ammonia in cell culture media was investigated through near-infrared spectroscopy of the 2.0–2.5 μm combination region. Samples taken from a three-day fibroblast culture were analyzed with standard clinical chemistry techniques and with a Fourier transform infrared spectrometer. The data were analyzed in three phases in an attempt to solve problems of correlation in the data. First, partial least-squares regression was used to build a multivariate calibration model based on the near-infrared spectra of the cell culture media and measured reference values. Second, an independent calibration was performed with aqueous mixtures of glucose, lactate, ammonia, glutamate, and glutamine. Finally, a calibration using a combination of spectra from cell culture media samples and aqueous mixtures was performed. In each case, the spectral loadings obtained from the calibration were inspected to determine the extent of weighting given to different spectral regions. The results indicate that a selective calibration model can be produced by combining data from samples of different type. Prediction errors of 2, 8, and 15% were obtained for glucose, lactate, and ammonia, respectively.

Anti-Inflammatory Effect of Near-Infrared Irradiated Cell Culture Media

2009 ◽  
Vol 29 (4) ◽  
pp. 338-344
Author(s):  
Sang-Gyung Kim ◽  
Im-Hee Shin ◽  
Chang-Hyuk Choi ◽  
Jung-Yoon Choe
Keyword(s):  
Cell Culture ◽  
Near Infrared ◽  
Culture Media ◽  
Cell Culture Media ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Investigation of Near-Infrared Spectroscopy for Periodic Determination of Glucose in Cell Culture Media in Situ

2000 ◽  
Vol 54 (10) ◽  
pp. 1453-1457 ◽  
Author(s):  
Christopher B. Lewis ◽  
Roger J. McNichols ◽  
Ashok Gowda ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Culture Media ◽  
Cell Culture Media

Cell culture media: NMR approaches to evaluating quality and nutrient consumption

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
KB Killday ◽  
AS Freund ◽  
C Fischer ◽  
KL Colson
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Cell Culture Media ◽  
Nutrient Consumption
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