Simultaneous Measurement of 19 Components in Serum-Containing Animal Cell Culture Media by Fourier Transform Near-Infrared Spectroscopy

2001 ◽  
Vol 17 (2) ◽  
pp. 376-378 ◽  
Author(s):  
Mark R. Riley ◽  
Heather M. Crider ◽  
Megan E. Nite ◽  
Rafael A. Garcia ◽  
Jennifer Woo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Simultaneous Measurement ◽  
Near Infrared ◽  
Culture Media ◽  
Animal Cell ◽  
Animal Cell Culture ◽  
Cell Culture Media

Near-Infrared Spectroscopy for Determination of Glucose, Lactate, and Ammonia in Cell Culture Media

1998 ◽  
Vol 52 (8) ◽  
pp. 1073-1078 ◽  
Author(s):  
Michael J. McShane ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Culture Media ◽  
Clinical Chemistry ◽  
Calibration Model ◽  
Aqueous Mixtures ◽  
Cell Culture Media ◽  
Combining Data

The potential for noninvasive determinations of glucose, lactate, and ammonia in cell culture media was investigated through near-infrared spectroscopy of the 2.0–2.5 μm combination region. Samples taken from a three-day fibroblast culture were analyzed with standard clinical chemistry techniques and with a Fourier transform infrared spectrometer. The data were analyzed in three phases in an attempt to solve problems of correlation in the data. First, partial least-squares regression was used to build a multivariate calibration model based on the near-infrared spectra of the cell culture media and measured reference values. Second, an independent calibration was performed with aqueous mixtures of glucose, lactate, ammonia, glutamate, and glutamine. Finally, a calibration using a combination of spectra from cell culture media samples and aqueous mixtures was performed. In each case, the spectral loadings obtained from the calibration were inspected to determine the extent of weighting given to different spectral regions. The results indicate that a selective calibration model can be produced by combining data from samples of different type. Prediction errors of 2, 8, and 15% were obtained for glucose, lactate, and ammonia, respectively.

Investigation of Near-Infrared Spectroscopy for Periodic Determination of Glucose in Cell Culture Media in Situ

2000 ◽  
Vol 54 (10) ◽  
pp. 1453-1457 ◽  
Author(s):  
Christopher B. Lewis ◽  
Roger J. McNichols ◽  
Ashok Gowda ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Culture Media ◽  
Cell Culture Media

Animal Cell Culture Media

2010 ◽  
Author(s):  
Natarajan Vijayasankaran ◽  
Jincai Li ◽  
Robert Shawley ◽  
Aaron Chen ◽  
Masaru Shiratori ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Animal Cell ◽  
Animal Cell Culture ◽  
Cell Culture Media

Animal Cell Culture Media

2003 ◽  
Author(s):  
Yung-Shyeng Tsao ◽  
Sandra L. Gould ◽  
David K. Robinson
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Animal Cell ◽  
Animal Cell Culture ◽  
Cell Culture Media

Colorimetric pH measurement of animal cell culture media

2010 ◽  
Vol 32 (11) ◽  
pp. 1599-1607 ◽  
Author(s):  
Juno Jang ◽  
Soo-Jin Moon ◽  
Sung-Hwan Hong ◽  
Ik-Hwan Kim
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Animal Cell ◽  
Animal Cell Culture ◽  
Ph Measurement ◽  
Cell Culture Media
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