Prediction of Key Analytes in Mammalian Cell Culture Media with On-Line near Infrared Spectroscopy

NIR news ◽  
2008 ◽  
Vol 19 (2) ◽  
pp. 9-11
Author(s):  
Robert A. Mattes ◽  
Denise Root
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Mammalian Cell ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Culture Media ◽  
Mammalian Cell Culture ◽  
Cell Culture Media ◽  
On Line

Semisynthetic model calibration for monitoring glucose in mammalian cell culture with in situ near infrared spectroscopy

2013 ◽  
Vol 111 (5) ◽  
pp. 896-903 ◽  
Author(s):  
Michael Milligan ◽  
Nicholas Lewin-Koh ◽  
Daniel Coleman ◽  
Adeyma Arroyo ◽  
Victor Saucedo
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Mammalian Cell ◽  
Near Infrared Spectroscopy ◽  
Model Calibration ◽  
Near Infrared ◽  
Mammalian Cell Culture

Near-Infrared Spectroscopy for Determination of Glucose, Lactate, and Ammonia in Cell Culture Media

1998 ◽  
Vol 52 (8) ◽  
pp. 1073-1078 ◽  
Author(s):  
Michael J. McShane ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Culture Media ◽  
Clinical Chemistry ◽  
Calibration Model ◽  
Aqueous Mixtures ◽  
Cell Culture Media ◽  
Combining Data

The potential for noninvasive determinations of glucose, lactate, and ammonia in cell culture media was investigated through near-infrared spectroscopy of the 2.0–2.5 μm combination region. Samples taken from a three-day fibroblast culture were analyzed with standard clinical chemistry techniques and with a Fourier transform infrared spectrometer. The data were analyzed in three phases in an attempt to solve problems of correlation in the data. First, partial least-squares regression was used to build a multivariate calibration model based on the near-infrared spectra of the cell culture media and measured reference values. Second, an independent calibration was performed with aqueous mixtures of glucose, lactate, ammonia, glutamate, and glutamine. Finally, a calibration using a combination of spectra from cell culture media samples and aqueous mixtures was performed. In each case, the spectral loadings obtained from the calibration were inspected to determine the extent of weighting given to different spectral regions. The results indicate that a selective calibration model can be produced by combining data from samples of different type. Prediction errors of 2, 8, and 15% were obtained for glucose, lactate, and ammonia, respectively.

Investigation of Near-Infrared Spectroscopy for Periodic Determination of Glucose in Cell Culture Media in Situ

2000 ◽  
Vol 54 (10) ◽  
pp. 1453-1457 ◽  
Author(s):  
Christopher B. Lewis ◽  
Roger J. McNichols ◽  
Ashok Gowda ◽  
Gerard L. Coté
Keyword(s):  
Cell Culture ◽  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Culture Media ◽  
Cell Culture Media

scholarly journals Detection of Glu-Glu-Tagged Proteins in Mammalian Cell Culture Media by Dot Immunoblotting

BioTechniques ◽  
2000 ◽  
Vol 28 (5) ◽  
pp. 934-937
Author(s):  
Jeff L. Ellsworth ◽  
Nels Hamacher ◽  
Nancy Bagwell ◽  
James W. West
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Culture Media ◽  
Mammalian Cell Culture ◽  
Cell Culture Media
Sign in / Sign up

Export Citation Format

Share Document