Rapid cell sheet detachment from alginate semi-interpenetrating nanocomposite hydrogels of PNIPAm and hectorite clay

2011 ◽  
Vol 71 (4) ◽  
pp. 447-454 ◽  
Author(s):  
Tao Wang ◽  
Dan Liu ◽  
Cuixia Lian ◽  
Shudian Zheng ◽  
Xinxing Liu ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Nanocomposite Hydrogels

Cell proliferation and cell sheet detachment from the positively and negatively charged nanocomposite hydrogels

Biopolymers ◽  
2013 ◽  
Vol 101 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Dan Liu ◽  
Tao Wang ◽  
Xinxing Liu ◽  
Zhen Tong
Keyword(s):  
Cell Proliferation ◽  
Cell Sheet ◽  
Nanocomposite Hydrogels

A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

1967 ◽  
Vol 25 ◽  
pp. 132-133
Author(s):  
R. Stephens ◽  
G. Schidlovsky ◽  
S. Kuzmic ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light Microscopy ◽  
Cell Sheet ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Electron Microscopic ◽  
Cell Sheets ◽  
Morphological Alterations ◽  
Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

Preparation of nanocomposite hydrogels with nanoclay: characterization, swelling properties and dye adsorption behavior

2020 ◽  
Vol 65 (2) ◽  
pp. 119-133
Author(s):  
Nesrimne SEDIIKI ◽  
◽  
Djamel ALIOUCHE ◽  
Imene BOUDHENE ◽  
◽  
...  
Keyword(s):  
Dye Adsorption ◽  
Adsorption Behavior ◽  
Swelling Properties ◽  
Nanocomposite Hydrogels

IN VITRO METHODS FOR THE STUDY OF THE ADENOHYPOPHYSIAL FUNCTIONS TO SECRETE GONADOTROPHIN

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S27-S40 ◽  
Author(s):  
T. Kobayashi ◽  
T. Kigawa ◽  
M. Mizuno ◽  
T. Watanabe
Keyword(s):  
Cell Culture ◽  
Organ Culture ◽  
Cultured Cells ◽  
Cell Sheet ◽  
Short Term ◽  
Hypothalamic Extract ◽  
Numerous Cell ◽  
Single Cell Culture ◽  
Almost All

ABSTRACT There are several in vitro methods to analyse the function of the adenohypophysis or the mechanisms of its regulation. The present paper deals with single cell culture, organ culture and short term incubation techniques by which the morphology and gonadotrophin-secreting function of the adenohypophysis were studied. In trypsin-dispersed cell culture, the adenohypophysial cells showed extensive propagation to form numerous cell colonies and finally develop into a confluent monolayer cell sheet covering completely the surface of culture vessels. Almost all of the cultured cells, however, became chromophobic, at least at the end of the first week of cultivation, when gonadotrophin was detectable neither in the culture medium nor in the cells themselves. After the addition of the hypothalamic extract, gonadotrophin became detectable again, and basophilic or PAS-positive granules also reappeared within the cells, suggesting that the gonadotrophs were stimulated by the extract to produce gonadotrophin. In organ culture and short term incubation, the incorporation of [3H] leucine into the adenohypophysial cells in relation to the addition of hypothalamic extract was examined. It was obvious that the ability to incorporate [3H] leucine into the gonadotrophs in vitro was highly dependent upon the presence of the hypothalamic extract.

Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction

Biomaterials ◽  
2014 ◽  
Vol 35 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Tsung-Szu Yeh ◽  
Yu-Hua Dean Fang ◽  
Chia-Hsin Lu ◽  
Shao-Chieh Chiu ◽  
Chia-Lin Yeh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Sheet ◽  
Myocardium Infarction ◽  
Adipose Derived Stem Cell
Sign in / Sign up

Export Citation Format

Share Document