scholarly journals Crystal Contact Engineering Enables Efficient Capture and Purification of an Oxidoreductase by Technical Crystallization

2020 ◽  
Vol 15 (11) ◽  
pp. 2000010
Author(s):  
Phillip Grob ◽  
Max Huber ◽  
Brigitte Walla ◽  
Johannes Hermann ◽  
Robert Janowski ◽  
...  
Keyword(s):  
Contact Engineering ◽  
Crystal Contact

Rational Crystal Contact Engineering ofLactobacillus brevisAlcohol Dehydrogenase To Promote Technical Protein Crystallization

2019 ◽  
Vol 19 (4) ◽  
pp. 2380-2387 ◽  
Author(s):  
Phillip Nowotny ◽  
Johannes Hermann ◽  
Jianing Li ◽  
Angela Krautenbacher ◽  
Kai Klöpfer ◽  
...  
Keyword(s):  
Protein Crystallization ◽  
Contact Engineering ◽  
Crystal Contact

Effective Improvement of D-Phenylglycine Aminotransferase Solubility by Protein Crystal Contact Engineering

2012 ◽  
Vol 22 (3) ◽  
pp. 147-155 ◽  
Author(s):  
Aiya Chantarasiri ◽  
Vithaya Meevootisom ◽  
Duangnate Isarangkul ◽  
Suthep Wiyakrutta
Keyword(s):  
Protein Crystal ◽  
Contact Engineering ◽  
Crystal Contact

scholarly journals Transfer of a Rational Crystal Contact Engineering Strategy between Diverse Alcohol Dehydrogenases

Crystals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 975
Author(s):  
Brigitte Walla ◽  
Daniel Bischoff ◽  
Robert Janowski ◽  
Nikolas von den Eichen ◽  
Dierk Niessing ◽  
...  
Keyword(s):  
High Throughput Screening ◽  
Crystal Packing ◽  
Protein Crystallization ◽  
Lactobacillus Brevis ◽  
Protein Crystal ◽  
Wild Type ◽  
Alcohol Dehydrogenases ◽  
Crystallization Conditions ◽  
Contact Engineering ◽  
Crystal Contact

Protein crystallization can serve as a purification step in biotechnological processes but is often limited by the non-crystallizability of proteins. Enabling or improving crystallization is mostly achieved by high-throughput screening of crystallization conditions and, more recently, by rational crystal contact engineering. Two selected rational crystal contact mutations, Q126K and T102E, were transferred from the alcohol dehydrogenases of Lactobacillus brevis (LbADH) to Lactobacillus kefir (LkADH). Proteins were expressed in E. coli and batch protein crystallization was performed in stirred crystallizers. Highly similar crystal packing of LkADH wild type compared to LbADH, which is necessary for the transfer of crystal contact engineering strategies, was achieved by aligning purification tag and crystallization conditions, as shown by X-ray diffraction. After comparing the crystal sizes after crystallization of LkADH mutants with the wild type, the mean protein crystal size of LkADH mutants was reduced by 40–70% in length with a concomitant increase in the total amount of crystals (higher number of nucleation events). Applying this measure to the LkADH variants studied results in an order of crystallizability T102E > Q126K > LkADH wild type, which corresponds to the results with LbADH mutants and shows, for the first time, the successful transfer of crystal contact engineering strategies.

scholarly journals Ultralow Specific Contact Resistivity in Metal–Graphene Junctions via Contact Engineering

2018 ◽  
Vol 6 (1) ◽  
pp. 1801285 ◽  
Author(s):  
Vikram Passi ◽  
Amit Gahoi ◽  
Enrique G. Marin ◽  
Teresa Cusati ◽  
Alessandro Fortunelli ◽  
...  
Keyword(s):  
Contact Resistivity ◽  
Specific Contact ◽  
Specific Contact Resistivity ◽  
Contact Engineering

Optimized Contact Engineering to Maximize Cell Current in NAND Flash Memory Technology

2013 ◽  
Vol 58 (5) ◽  
pp. 103-108
Author(s):  
J. H. Yoon ◽  
H.-S. Kim ◽  
Y. S. Kim ◽  
J. K. Lee ◽  
E. S. Han ◽  
...  
Keyword(s):  
Flash Memory ◽  
Nand Flash ◽  
Nand Flash Memory ◽  
Cell Current ◽  
Contact Engineering

scholarly journals Crystal contact-free conformation of an intrinsically flexible loop in protein crystal: Tim21 as the case study

2020 ◽  
Vol 1864 (2) ◽  
pp. 129418 ◽  
Author(s):  
Siqin Bala ◽  
Shoko Shinya ◽  
Arpita Srivastava ◽  
Marie Ishikawa ◽  
Atsushi Shimada ◽  
...  
Keyword(s):  
Protein Crystal ◽  
Flexible Loop ◽  
Contact Free ◽  
Crystal Contact
Sign in / Sign up

Export Citation Format

Share Document