Modulation of the Purine Pathway for Riboflavin Production in Flavinogenic Recombinant Strain of the Yeast Candida famata

2020 ◽  
Vol 15 (7) ◽  
pp. 1900468
Author(s):  
Kostyantyn V. Dmytruk ◽  
Justyna Ruchala ◽  
Daria V. Fedorovych ◽  
Roman D. Ostapiv ◽  
Andriy A. Sibirny
Keyword(s):  
Recombinant Strain ◽  
Riboflavin Production ◽  
Candida Famata ◽  
Purine Pathway

scholarly journals Metabolic Engineering of the Purine Pathway for Riboflavin Production in Ashbya gossypii

2005 ◽  
Vol 71 (10) ◽  
pp. 5743-5751 ◽  
Author(s):  
Alberto Jiménez ◽  
María A. Santos ◽  
Markus Pompejus ◽  
José L. Revuelta
Keyword(s):  
De Novo ◽  
Feedback Inhibition ◽  
Site Directed Mutagenesis ◽  
Transcriptional Level ◽  
Ashbya Gossypii ◽  
Purine Nucleotides ◽  
Phosphoribosyl Pyrophosphate ◽  
Triple Mutant ◽  
Riboflavin Production ◽  
Purine Pathway

ABSTRACT Purine nucleotides are essential precursors for living organisms because they are involved in many important processes, such as nucleic acid synthesis, energy supply, and the biosynthesis of several amino acids and vitamins such as riboflavin. GTP is the immediate precursor for riboflavin biosynthesis, and its formation through the purine pathway is subject to several regulatory mechanisms in different steps. Extracellular purines repress the transcription of most genes required for de novo ATP and GTP synthesis. Additionally, three enzymes of the pathway, phosphoribosyl pyrophosphate (PRPP) amidotransferase, adenylosuccinate synthetase, and IMP dehydrogenase, are subject to feedback inhibition by their end products. Here we report the characterization and manipulation of the committed step in the purine pathway of the riboflavin overproducer Ashbya gossypii. We report that phosphoribosylamine biosynthesis in A. gossypii is negatively regulated at the transcriptional level by extracellular adenine. Furthermore, we show that ATP and GTP exert a strong inhibitory effect on the PRPP amidotransferase from A. gossypii. We constitutively overexpressed the AgADE4 gene encoding PRPP amidotransferase in A. gossypii, thereby abolishing the adenine-mediated transcriptional repression. In addition, we replaced the corresponding residues (aspartic acid310, lysine333, and alanine417) that have been described to be important for PRPP amidotransferase feedback inhibition in other organisms by site-directed mutagenesis. With these manipulations, we managed to enhance metabolic flow through the purine pathway and to increase the production of riboflavin in the triple mutant strain 10-fold (228 mg/liter).

Induced Biofilm Cultivation Enhances Riboflavin Production by an Intertidally Derived Candida famata

2012 ◽  
Vol 166 (8) ◽  
pp. 1991-2006 ◽  
Author(s):  
Sayani Mitra ◽  
Dheeraj Thawrani ◽  
Priyam Banerjee ◽  
Ratan Gachhui ◽  
Joydeep Mukherjee
Keyword(s):  
Riboflavin Production ◽  
Candida Famata

Metabolic engineering and classic selection of the yeast Candida famata (Candida flareri) for construction of strains with enhanced riboflavin production

2011 ◽  
Vol 13 (1) ◽  
pp. 82-88 ◽  
Author(s):  
Kostyantyn V. Dmytruk ◽  
Valentyna Y. Yatsyshyn ◽  
Natalia O. Sybirna ◽  
Daria V. Fedorovych ◽  
Andriy A. Sibirny
Keyword(s):  
Metabolic Engineering ◽  
Riboflavin Production ◽  
Candida Famata ◽  
Selection Of

Construction and fed-batch cultivation of Candida famata with enhanced riboflavin production

2014 ◽  
Vol 172 ◽  
pp. 11-17 ◽  
Author(s):  
Kostyantyn Dmytruk ◽  
Oleksy Lyzak ◽  
Valentyna Yatsyshyn ◽  
Maciej Kluz ◽  
Vladimir Sibirny ◽  
...  
Keyword(s):  
Batch Cultivation ◽  
Fed Batch ◽  
Riboflavin Production ◽  
Candida Famata ◽  
Fed Batch Cultivation

Enhancing riboflavin production by genetic modification of purine pathway in Bacillus subtilis

2008 ◽  
Vol 136 ◽  
pp. S35-S36
Author(s):  
Shuobo Shi ◽  
Tao Chen ◽  
Xun Chen ◽  
Qiuli Wu ◽  
Yu Gan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Modification ◽  
Riboflavin Production ◽  
Purine Pathway

Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida famata

2021 ◽  
pp. 31-42
Author(s):  
Andriy O. Tsyrulnyk ◽  
Dariya V. Fedorovych ◽  
Kostyantyn V. Dmytruk ◽  
Andriy A. Sibirny
Keyword(s):  
Riboflavin Production ◽  
Candida Famata
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