Using fermentation waste of ethanol‐producing yeast for bacterial riboflavin production and recycling of spent bacterial mass for enhancing the growth of oily plants

2021 ◽  
Author(s):  
Shymaa Ryhan Bashandy ◽  
Mohamed Hemida Abd‐Alla ◽  
Ghada Abd‐Elmonsef Mahmoud
Keyword(s):  
Riboflavin Production ◽  
Bacterial Mass

scholarly journals Dynamic Co-Cultivation Process of Corynebacterium glutamicum Strains for the Fermentative Production of Riboflavin

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Fernando Pérez-García ◽  
Arthur Burgardt ◽  
Dina R. Kallman ◽  
Volker F. Wendisch ◽  
Nadav Bar
Keyword(s):  
Corynebacterium Glutamicum ◽  
Xanthomonas Campestris ◽  
Product Formation ◽  
Volumetric Productivity ◽  
Riboflavin Production ◽  
Xylose Consumption ◽  
Single Strain ◽  
Sugar Mixtures ◽  
Fed Batch Fermentation ◽  
Overexpressing Strain

Residual streams from lignocellulosic processes contain sugar mixtures of glucose, xylose, and mannose. Here, the industrial workhorse Corynebacterium glutamicum was explored as a research platform for the rational utilization of a multiple sugar substrate. The endogenous manA gene was overexpressed to enhance mannose utilization. The overexpression of the xylA gene from Xanthomonas campestris in combination with the endogenous xylB gene enabled xylose consumption by C. glutamicum. Furthermore, riboflavin production was triggered by overexpressing the sigH gene from C. glutamicum. The resulting strains were studied during batch fermentations in flasks and 2 L lab-scale bioreactors separately using glucose, mannose, xylose, and a mixture of these three sugars as a carbon source. The production of riboflavin and consumption of sugars were improved during fed-batch fermentation thanks to a dynamic inoculation strategy of manA overexpressing strain and xylAB overexpressing strain. The final riboflavin titer, yield, and volumetric productivity from the sugar mixture were 27 mg L−1, 0.52 mg g−1, and 0.25 mg L−1 h−1, respectively. It reached a 56% higher volumetric productivity with 45% less by-product formation compared with an equivalent process inoculated with a single strain overexpressing the genes xylAB and manA combined. The results indicate the advantages of dynamic multi strains processes for the conversion of sugar mixtures.

scholarly journals Riboflavin Production in Lactococcus lactis: Potential for In Situ Production of Vitamin-Enriched Foods

2004 ◽  
Vol 70 (10) ◽  
pp. 5769-5777 ◽  
Author(s):  
Catherine Burgess ◽  
Mary O'Connell-Motherway ◽  
Wilbert Sybesma ◽  
Jeroen Hugenholtz ◽  
Douwe van Sinderen
Keyword(s):  
Functional Analysis ◽  
Lactic Acid ◽  
Lactococcus Lactis ◽  
Regulatory Region ◽  
Northern Hybridization ◽  
Vitamin B ◽  
Fermented Foods ◽  
Riboflavin Production ◽  
In Situ Production

ABSTRACT This study describes the genetic analysis of the riboflavin (vitamin B2) biosynthetic (rib) operon in the lactic acid bacterium Lactococcus lactis subsp. cremoris strain NZ9000. Functional analysis of the genes of the L. lactis rib operon was performed by using complementation studies, as well as by deletion analysis. In addition, gene-specific genetic engineering was used to examine which genes of the rib operon need to be overexpressed in order to effect riboflavin overproduction. Transcriptional regulation of the L. lactis riboflavin biosynthetic process was investigated by using Northern hybridization and primer extension, as well as the analysis of roseoflavin-induced riboflavin-overproducing L. lactis isolates. The latter analysis revealed the presence of both nucleotide replacements and deletions in the regulatory region of the rib operon. The results presented here are an important step toward the development of fermented foods containing increased levels of riboflavin, produced in situ, thus negating the need for vitamin fortification.

Application of the Mono Model to Describe the Periodic Process of Bacterial Mass Growth on Concrete Surfaces

2021 ◽  
Author(s):  
Elena S. Dergunova ◽  
Tatiana I. Gubina ◽  
Elizaveta A. Guryanova ◽  
Margarita A. Goncharova
Keyword(s):  
Periodic Process ◽  
Mass Growth ◽  
Bacterial Mass

Effects of sirtuins on the riboflavin production in Ashbya gossypii

2021 ◽  
Author(s):  
Tatsuya Kato ◽  
Junya Azegami ◽  
Mai Kano ◽  
Hesham A. El Enshasy ◽  
Enoch Y. Park
Keyword(s):  
Ashbya Gossypii ◽  
Riboflavin Production

scholarly journals Medium composition influence on Biotin and Riboflavin production by newly isolated Candida sp

2011 ◽  
Vol 42 (3) ◽  
pp. 1093-1100 ◽  
Author(s):  
Gaby Tiemi Suzuki ◽  
Juliana Alves Macedo ◽  
Gabriela Alves Macedo
Keyword(s):  
Medium Composition ◽  
Riboflavin Production ◽  
Candida Sp

scholarly journals Influence of siderophores and iron on Mycobacterium bovis isolation from pathological material

2021 ◽  
Vol 7 (1-2) ◽  
pp. 21-26
Author(s):  
A. I. Zavgorodniy ◽  
S. A. Pozmogova ◽  
V. V. Bilushko ◽  
Kalashnyk Kalashnyk ◽  
O. I. Gologurska
Keyword(s):  
Mycobacterium Bovis ◽  
Culture Filtrate ◽  
Nutrient Medium ◽  
Culture Media ◽  
Alcoholic Extract ◽  
Simultaneous Presence ◽  
Growth Properties ◽  
Pathological Material ◽  
Bacterial Mass

The article presents the results of studying the effect of siderophores and iron on the isolation of Mycobacterium bovis from pathological material. It has been established that the simultaneous presence of iron and siderophore from M. phlei in the nutrient medium makes it possible to detect the growth of M. bovis from pathological material 6–8 days earlier; ensures the growth of more colonies and bacterial mass. The presence of heterologous to mycobacteria siderophore (from Nocardia spp.) in the medium reduces the elective (growth) properties of the medium. Siderophores found in the culture filtrate or alcoholic extract of M. phlei can be valuable additives to culture media for the accelerated isolation of M. bovis from pathological material

Effect of Gum Arabic on the Fecal Bacterial Mass in Healthy Human Volunteers

Gum Arabic ◽  
2018 ◽  
pp. 297-304
Author(s):  
Rasheeda Hamid Abdalla Ahmed ◽  
Abdalbasit Adam Mariod ◽  
Sulieman Mohamed El Sanousi
Keyword(s):  
Gum Arabic ◽  
Healthy Human ◽  
Human Volunteers ◽  
Bacterial Mass
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