Chemiluminescence selectivity enhancement in the on-chip Ru(bpy)3 2+ system: The potential role of buffer type and pH in the determination of hydrochlorothiazide in combined formulations and human plasma

Luminescence ◽  
2017 ◽  
Vol 32 (8) ◽  
pp. 1494-1503 ◽  
Author(s):  
Afsal Mohammed Kadavilpparampu ◽  
Haider A.J. Al Lawati ◽  
Fakhr Eldin O. Suliman
Keyword(s):  
Human Plasma ◽  
Potential Role ◽  
On Chip

Simultaneous determination of multiple cytokines reveals a pro-inflammatory and pro-angiogenic signature after major cardiothoracic surgery: Potential role of C-reactive protein

Cytokine ◽  
2012 ◽  
Vol 60 (3) ◽  
pp. 593-595 ◽  
Author(s):  
Veronica Tisato ◽  
Lorenzo Monasta ◽  
Gianni Biolo ◽  
Francesco Donatelli ◽  
Paola Secchiero ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Potential Role ◽  
Cardiothoracic Surgery ◽  
C Reactive Protein ◽  
Reactive Protein

Confirmation of hybridity using GISH and determination of 18S rDNA copy number using FISH in interspecific F1 hybrids of Tecoma (Bignoniaceae)

Genome ◽  
2012 ◽  
Vol 55 (6) ◽  
pp. 437-445 ◽  
Author(s):  
Ryan N. Contreras ◽  
John M. Ruter ◽  
Joann Conner ◽  
Yajuan Zeng ◽  
Peggy Ozias-Akins
Keyword(s):  
Internal Transcribed Spacer ◽  
Potential Role ◽  
F1 Hybrids ◽  
Internal Transcribed Spacer Region ◽  
Pistillate Parent ◽  
Fertile Hybrids ◽  
Species Hybrids ◽  
Rdna Copy

Interspecific hybridization in Tecoma Juss. was conducted to develop novel forms for the nursery industry. We report fertile hybrids from the cross T. garrocha Hieron. (pistillate parent) × T. stans (L.) Juss. ex Kunth. Leaf morphology of the F1 hybrids of T. garrocha × T. stans was intermediate between the parents. GISH also confirmed hybridity. The F1 hybrids were successfully backcrossed to both parents and self-pollinated to produce BC and F2 progeny. Tecoma garrocha , T. stans, and T. guarume A. DC. ‘Tangelo’ were self-fertile. The F1 hybrids also were crossed with T. capensis (Thunb.) Lindl. and T. guarume ‘Tangelo’, resulting in three-species hybrids. FISH conducted on F1 hybrids identified four copies of the 18S internal transcribed spacer region. Further analysis using FISH has the potential to provide information on the evolution of Bignoniaceae and the potential role of polyploidy.

Ion-chromatographic determination of L-tartrate in urine samples

1991 ◽  
Vol 37 (1) ◽  
pp. 90-93 ◽  
Author(s):  
Mlchele Petrarulo ◽  
Martino Marangella ◽  
Omalla Bianco ◽  
Franco Linari
Keyword(s):  
Potential Role ◽  
Signal To Noise Ratio ◽  
Standard Procedure ◽  
Dicarboxylic Acids ◽  
Chromatographic Determination ◽  
Signal To Noise ◽  
Coefficients Of Variation ◽  
Analytical Recovery

Abstract We propose using ion chromatography to determine tartrate concentration in urine. A 100-microL sample of urine is diluted and injected into the chromatograph. Tartrate is eluted within 11.5 min as a distinct and well-resolved peak. The sensitivity of the standard procedure (signal-to-noise ratio, 3/1) is 30 mumol/L. The intra-run and interrun coefficients of variation are 2.5% and 4.1%, respectively. Mean analytical recovery of known amounts of added tartrate ranges between 94.2% and 104.0%. We investigated the specificity of the procedure by analyzing urine containing added dicarboxylic acids structurally related to tartrate. The reliability of the procedure makes it suitable for investigating tartrate metabolism, e.g., the potential role of tartrate as an inhibitor of crystallization in calcium nephrolithiasis.

scholarly journals The blood resistin level in patients with DM2, depending on the duration of the disease

2015 ◽  
Vol 28 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Alina Urbanovych
Keyword(s):  
Type 2 Diabetes ◽  
Blood Level ◽  
Potential Role ◽  
Control Group ◽  
Resistin Level ◽  
Group I ◽  
Significant Difference

Abstract Resistin is still a little known hormone of the adipose tissue. The potential role of resistin in the development of DM2 has been currently investigated. The aim of our study was to detect the resistin blood level in patients with DM2, depending on the duration of the disease. In so doing, a determination of resistin and insulin blood level was conducted in 305 patients with DM2, and in a control group of 32 persons. Before testing, the patients were placed into four groups, depending on the duration of type 2 diabetes. Our results indicate that the resistin level was significantly lower in the control group of patients, in comparison with the DM2 patients groups. Moreover, a significantly lower resistin level was found in group I (firstly diagnosed DM2), in comparison with the groups of patients with a different duration of DM2. No correlation between resistin level and BMI, and between resistin and insulin blood level was found. However, a tendency towards increase of resistin blood level is noticeably evident in co-relation with increment of DM2 duration. In addition, the resistin level was considerably lower in patients with no DM2, when compared with patients with diagnosed DM2. Yet, there was no significant difference in the resistin blood level depending on the sex of the patients at the same duration of DM2

A New Method for the Determination of Antiplasmin and Antiactivator Activity in Plasma. The Role of these Inhibitors in Controlling the Lysis of Fibrinogen and Fibrin

1979 ◽  
Author(s):  
B. Lipinski ◽  
T. Nunn ◽  
V. Gurewich
Keyword(s):  
Human Plasma ◽  
Linear Relation ◽  
Borate Buffer ◽  
Lytic Activity ◽  
New Method ◽  
Semiquantitative Determination ◽  
Circular Zone ◽  
Total Inhibition

In order to evaluate the effect of plasma inhibitors on the lysis of fibrinogen (F) and fibrin (f) by UK and an activator derived from euglobulin, agarose (1%) plates in borate buffer (pH 7.7) containing human F (0.1%) were prepared. Fibrinolysis was measured after incubation (18h., 37°C) on plates clotted with Arvin (10u.). Fibrinogenolysis was measured using unclotted plates to which Arvin and EACA (0.01M) were added after incubation with the activators. Lytic activity was determined by measuring the clear circular zone around the wells containing the activators. In both the F and f plates, a linear relation between lysis zones and UK concentrations (0-100u/ml) was found. Inhibitors in human plasma (HP) were evaluated by incubating (15 min.) activators with HP prior to their application to the plates. This resulted in a slight (10-20%) reduction in lysis on the f plates and total inhibition of lysis on the F plates. By contrast, when plasmin was mixed with HP, the lysis zones on the F and f plates were identical. It was concluded that an antiactivator rather than an antiplasmin is responsible for the inhibition of F-lysis. These F and f agarose plates provide a practical means for the semiquantitative determination of antiactivators and antiplasmins in patient plasma.

Determination of CSF shunt patency with a lumbar infusion test

1983 ◽  
Vol 58 (4) ◽  
pp. 553-556 ◽  
Author(s):  
Hart Schutz ◽  
Karel G. Ter Brugge ◽  
Ming C. Chiu ◽  
Angele Mongul ◽  
Ferelith Taylor
Keyword(s):  
Potential Role ◽  
Csf Shunt ◽  
Csf Dynamics ◽  
Content Type ◽  
Lumbar Infusion Test ◽  
Method Of Assessment ◽  
Infusion Technique ◽  
Tomography Scanning

✓ The authors report on the potential role of the lumbar infusion technique in the determination of cerebrospinal fluid (CSF) shunt patency. The test was performed in a series of 12 patients and reliably predicted shunt patency. This method of assessment proved to be superior to computerized tomography scanning because of its ability to demonstrate the CSF dynamics rather than the ventricular size.

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