Confirmation of hybridity using GISH and determination of 18S rDNA copy number using FISH in interspecific F1 hybrids of Tecoma (Bignoniaceae)

Genome ◽  
2012 ◽  
Vol 55 (6) ◽  
pp. 437-445 ◽  
Author(s):  
Ryan N. Contreras ◽  
John M. Ruter ◽  
Joann Conner ◽  
Yajuan Zeng ◽  
Peggy Ozias-Akins
Keyword(s):  
Internal Transcribed Spacer ◽  
Potential Role ◽  
F1 Hybrids ◽  
Internal Transcribed Spacer Region ◽  
Pistillate Parent ◽  
Fertile Hybrids ◽  
Species Hybrids ◽  
Rdna Copy

Interspecific hybridization in Tecoma Juss. was conducted to develop novel forms for the nursery industry. We report fertile hybrids from the cross T. garrocha Hieron. (pistillate parent) × T. stans (L.) Juss. ex Kunth. Leaf morphology of the F1 hybrids of T. garrocha × T. stans was intermediate between the parents. GISH also confirmed hybridity. The F1 hybrids were successfully backcrossed to both parents and self-pollinated to produce BC and F2 progeny. Tecoma garrocha , T. stans, and T. guarume A. DC. ‘Tangelo’ were self-fertile. The F1 hybrids also were crossed with T. capensis (Thunb.) Lindl. and T. guarume ‘Tangelo’, resulting in three-species hybrids. FISH conducted on F1 hybrids identified four copies of the 18S internal transcribed spacer region. Further analysis using FISH has the potential to provide information on the evolution of Bignoniaceae and the potential role of polyploidy.

Simultaneous determination of multiple cytokines reveals a pro-inflammatory and pro-angiogenic signature after major cardiothoracic surgery: Potential role of C-reactive protein

Cytokine ◽  
2012 ◽  
Vol 60 (3) ◽  
pp. 593-595 ◽  
Author(s):  
Veronica Tisato ◽  
Lorenzo Monasta ◽  
Gianni Biolo ◽  
Francesco Donatelli ◽  
Paola Secchiero ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Potential Role ◽  
Cardiothoracic Surgery ◽  
C Reactive Protein ◽  
Reactive Protein

Ion-chromatographic determination of L-tartrate in urine samples

1991 ◽  
Vol 37 (1) ◽  
pp. 90-93 ◽  
Author(s):  
Mlchele Petrarulo ◽  
Martino Marangella ◽  
Omalla Bianco ◽  
Franco Linari
Keyword(s):  
Potential Role ◽  
Signal To Noise Ratio ◽  
Standard Procedure ◽  
Dicarboxylic Acids ◽  
Chromatographic Determination ◽  
Signal To Noise ◽  
Coefficients Of Variation ◽  
Analytical Recovery

Abstract We propose using ion chromatography to determine tartrate concentration in urine. A 100-microL sample of urine is diluted and injected into the chromatograph. Tartrate is eluted within 11.5 min as a distinct and well-resolved peak. The sensitivity of the standard procedure (signal-to-noise ratio, 3/1) is 30 mumol/L. The intra-run and interrun coefficients of variation are 2.5% and 4.1%, respectively. Mean analytical recovery of known amounts of added tartrate ranges between 94.2% and 104.0%. We investigated the specificity of the procedure by analyzing urine containing added dicarboxylic acids structurally related to tartrate. The reliability of the procedure makes it suitable for investigating tartrate metabolism, e.g., the potential role of tartrate as an inhibitor of crystallization in calcium nephrolithiasis.

scholarly journals Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil

2016 ◽  
Vol 46 (6) ◽  
pp. 943-949 ◽  
Author(s):  
Geraldo Salgado-Neto ◽  
Marcos André Braz Vaz ◽  
Jerson Vanderlei Carús Guedes ◽  
Marlove Fátima Brião Muniz ◽  
Elena Blume
Keyword(s):  
Fusarium Oxysporum ◽  
Root Rot ◽  
Internal Transcribed Spacer ◽  
Alimentary Canal ◽  
First Record ◽  
Economic Importance ◽  
Species Level ◽  
Internal Transcribed Spacer Region ◽  
Root Pathogens

ABSTRACT: The scientific and economic importance of soybean root rot justifies the study of this disease, especially the role of insects as dispersers in the spread of root pathogens. The objective was to identify and evaluate qualitatively and quantitatively the role of Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus(Coleoptera: Melolonthidae) in the dispersion of root pathogens in cultivated fields. The fungi were identified to the species level with DNA extraction and sequencing of these organisms within Melolonthidae larvae. Larvae were dissected and separated into the parts: mouth parts, prothorax, cuticle and digestive tract (alimentary canal). The Internal Transcribed Spacer region of the rRNA was amplified and compared to sequences deposited in GenBank. This is the first record of dispersion Fusarium oxysporumby Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus (Coleoptera: Melolonthidae) under soybean in Brazil.

Determination of a Minimal DNA Sequence of the Internal Transcribed Spacer Region for the in silico Identification of Botryosphaeria sp.

2007 ◽  
Vol 2 (3) ◽  
pp. 201-210
Author(s):  
L.A. Vilas-Boas . ◽  
M.A. Coronado . ◽  
G.T. Vilas-Boas . ◽  
R.F.H. Dekker . ◽  
A.M. Barbosa . ◽  
...  
Keyword(s):  
Dna Sequence ◽  
In Silico ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region ◽  
In Silico Identification

Genetic diversity of Bartonella genotypes found in the striped field mouse (Apodemus agrarius) in Central Europe

Parasitology ◽  
2016 ◽  
Vol 143 (11) ◽  
pp. 1437-1442 ◽  
Author(s):  
JASNA KRALJIK ◽  
ANNA PAZIEWSKA-HARRIS ◽  
DANA MIKLISOVÁ ◽  
LUCIA BLAŇAROVÁ ◽  
LADISLAV MOŠANSKÝ ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Recombination Event ◽  
Housekeeping Genes ◽  
23S Rrna ◽  
Internal Transcribed Spacer Region ◽  
Apodemus Agrarius ◽  
The Past ◽  
Potential Recombination Event ◽  
Past 1000 Years

SUMMARYWe investigated the diversity of Bartonella in Apodemus agrarius, an important rodent of peri-domestic habitats, which has spread into Europe in the past 1000 years. Spleen samples of 344 A. agrarius from Eastern Slovakia were screened for the presence of Bartonella spp. using 16S–23S rRNA internal transcribed spacer region and bacteria were detected in 9% of rodents. Based on sequencing of three housekeeping genes (gltA, rpoB and groEL) Bartonella genotypes were ascribed to the species typical for mice and voles: B. grahamii, B. taylorii and B. birtlesii. However, the study also confirmed presence of genotypes belonging to the B. clarridgeiae/B. rochalimae clade, and the B. elizabethae/B. tribocorum clade, which are not commonly found in woodland rodents. In addition, a potential recombination event between these two genotypes was noted, which highlights an important role of A. agrarius in shaping Bartonella diversity and evolution.

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