Comparative pharmacokinetics and tissue distribution of cryptotanshinone, tanshinone IIA, dihydrotanshinone I, and tanshinone I after oral administration of pure tanshinones and liposoluble extract of Salvia miltiorrhiza to rats

2020 ◽  
Vol 41 (1-2) ◽  
pp. 54-63 ◽  
Author(s):  
Daifei Wang ◽  
Weibang Yu ◽  
Lin Cao ◽  
Chuncao Xu ◽  
Guoyao Tan ◽  
...  
Keyword(s):  
Oral Administration ◽  
Tissue Distribution ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Tanshinone I

scholarly journals Metabolism of Tanshinone IIA, Cryptotanshinone and Tanshinone I from Radix Salvia Miltiorrhiza in Zebrafish

Molecules ◽  
2012 ◽  
Vol 17 (7) ◽  
pp. 8617-8632 ◽  
Author(s):  
Yingjie Wei ◽  
Ping Li ◽  
Changmei Wang ◽  
Yunru Peng ◽  
Luan Shu ◽  
...  
Keyword(s):  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Tanshinone I ◽  
Radix Salvia Miltiorrhiza

Enhancement of solubility and dissolution rate of cryptotanshinone, tanshinone I and tanshinone IIA extracted from Salvia miltiorrhiza

2012 ◽  
Vol 35 (8) ◽  
pp. 1457-1464 ◽  
Author(s):  
Hang Yu ◽  
Robhash Kusam Subedi ◽  
Pushp R. Nepal ◽  
Yoon G. Kim ◽  
Hoo-Kyun Choi
Keyword(s):  
Dissolution Rate ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Tanshinone I

scholarly journals Quantitative Determination and Validation of Four Ketones in Salvia miltiorrhiza Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2043
Author(s):  
Yuanyuan Li ◽  
Zhuoni Hou ◽  
Feng Su ◽  
Jipeng Chen ◽  
Xiaodan Zhang ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Salvia Miltiorrhiza Bunge ◽  
Simple Method ◽  
Tanshinone I

Salvia mltiorrhiza Bunge (SMB) is native to China, whose dried root has been used as medicine. A few chromatographic- or spectrometric-based methods have already been used to analyze the lipid-soluble components in SMB. However, the methodology of qNMR on the extracts of fresh SMB root has not been verified so far. The purpose of this study was to establish a fast and simple method to quantify the tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh Salvia Miltiorrhiza Bunge root without any pre-purification steps using 1H-NMR spectroscopy. The process is as follows: first, 70% methanol aqueous extracts of fresh Salvia Miltiorrhiza Bunge roots were quantitatively analyzed for tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone using 1H-NMR spectroscopy. Different internal standards were tested and the validated method was compared with HPLC. 3,4,5-trichloropyridine was chosen as the internal standard. Twelve samples of Salvia Miltiorrhiza Bunge were quantitatively analyzed by qNMR and HPLC respectively. Then, the results were analyzed by chemometric approaches. This NMR method offers a fast, stable, and accurate analysis of four ketones: tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh roots of Salvia Miltiorrhiza Bunge.

Tanshinone IIA and tanshinone I production by Trichoderma atroviride D16, an endophytic fungus in Salvia miltiorrhiza

Phytomedicine ◽  
2012 ◽  
Vol 19 (3-4) ◽  
pp. 330-333 ◽  
Author(s):  
Qianliang Ming ◽  
Ting Han ◽  
Wenchao Li ◽  
Qiaoyan Zhang ◽  
Hong Zhang ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Trichoderma Atroviride ◽  
Tanshinone I

scholarly journals Detection-confirmation-standardization-quantification: a novel method for the quantitative analysis of active components in traditional Chinese medicines

2021 ◽  
Vol 3 ◽  
pp. e10
Author(s):  
Xiaohong Song ◽  
Anyi Zhao ◽  
Yan Liu ◽  
Jintang Cheng ◽  
Sha Chen ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Preparative Hplc ◽  
Traditional Chinese Medicines ◽  
Reference Standard ◽  
Active Components ◽  
Chinese Medicines ◽  
Tanshinone I ◽  
Novel Method

Background Quantitative analysis of the active ingredients of Traditional Chinese Medicine is a research tendency. The objective of this study was to build a novel method, namely, Detection-confirmation-standardization-quantification (DCSQ), for the quantitative analysis of active components in traditional Chinese medicines, without individual reference standard. Methods Danshen (the dried root of Radix Salvia miltiorrhiza) was used as the matrix. The “extraction” function in high-performance liquid chromatography-mass (HPLC-MS) instrument was used to find the peaks corresponding to cryptotanshinone, tanshinone I, and tanshinone IIA in the total ion current (TIC) chromatogram of Danshen. The multicomponent reference standard (MCRS) containing the three tanshinones mainly was prepared by preparative HPLC. The contents of them in the resulting MCRS were determined by NMR; moreover, the constituents of the MCRS were confirmed. The MCRS containing known content of the three tanshinones was used as the reference standard for the quantitative analysis of cryptotanshinone, tanshinone I and tanshinone IIA in Danshen Samples by analytical HPLC. Results The optimized HPLC conditions for the quantitative analysis of the active components in Danshen were established, and the assignments of the extracted peaks were confirmed by analyzing the characteristic fragments in their MS/MS product ion spectra and UV spectra. Then, the MCRS containing the three tanshinones were successfully prepared. The results of determination about the contents by NMR showed linearity fitted with high likelihood and calibration curves possessed high linearity. The results of determination on Danshen Samples obtained through DCSQ exhibited minimal deviations, in contrast to those obtained through individual reference standards. Conclusion The establishing DCSQ is independent and convenient for the quantitative analysis of the active components in TCMs by MCRS, without individual reference standard. This method is a great advance in quantitative analysis for complex composition, especially TCMs.

scholarly journals Monoamine Oxidase Inhibition by Major Tanshinones From Salvia Miltiorrhiza and Selective Muscarinic Acetylcholine M4 Receptor Antagonism by Tanshinone I

2021 ◽  
Author(s):  
Ritu Prajapati ◽  
Se Eun Park ◽  
Su Hui Seong ◽  
Pradeep Paudel ◽  
Fazlin Mohd Fauzi ◽  
...  
Keyword(s):  
Monoamine Oxidase ◽  
Enzyme Inhibition ◽  
Acetylcholine Receptors ◽  
Salvia Miltiorrhiza ◽  
Muscarinic Acetylcholine Receptors ◽  
Motor Dysfunction ◽  
Tanshinone Iia ◽  
Tanshinone I ◽  
Muscarinic Acetylcholine

Abstract Monoamine oxidases (MAOs) and muscarinic acetylcholine receptors (mAChRs) are considered important therapeutic targets for Parkinson's disease (PD). Lipophilic tanshinones are major phytoconstituents in dried roots of Salvia miltiorrhiza that have demonstrated neuroprotective anti-Parkinson effects against dopaminergic neurotoxins and the inhibition of human monoamine oxidase (hMAO)-A. Since MAO-B inhibition is also considered an important therapeutic approach for PD, we accessed the potential of three abundant tanshinone congeners in hMAO-A and hMAO-B inhibition in vitro. In our study, tanshinone I (1) exhibited the highest potency against hMAO-A, followed by tanshinone IIA and cryptotanshinone with IC50 values less than 10 μM. They also suppressed hMAO-B activity with IC50 values lower than 25 μM. Although the hMAO-A inhibitory activity of tanshinones has been reported, the mechanism of enzyme inhibition and binding sites are yet to be studied. Thus, we conducted enzyme kinetics and molecular docking studies to evaluate the mode of enzyme inhibition and interactions. Proteochemometric modeling further predicted mAChRs as potential targets of 1 and an in vitro functional G-protein coupled receptor assay confirmed the selective M4 antagonist nature of 1. These findings indicate that 1 is a potential therapeutic molecule for managing the motor dysfunction and depression associated with PD.

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