Determination of tanshinone I, tanshinone IIA, and cryptotanshinone in Salvia miltiorrhiza bunge and urine sample by HPLC

2010 ◽  
pp. n/a-n/a
Author(s):  
Minglei Tian ◽  
Kyung Ho Row
Keyword(s):  
Urine Sample ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Salvia Miltiorrhiza Bunge ◽  
Tanshinone I

scholarly journals Quantitative Determination and Validation of Four Ketones in Salvia miltiorrhiza Bunge Using Quantitative Proton Nuclear Magnetic Resonance Spectroscopy

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2043
Author(s):  
Yuanyuan Li ◽  
Zhuoni Hou ◽  
Feng Su ◽  
Jipeng Chen ◽  
Xiaodan Zhang ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Salvia Miltiorrhiza Bunge ◽  
Simple Method ◽  
Tanshinone I

Salvia mltiorrhiza Bunge (SMB) is native to China, whose dried root has been used as medicine. A few chromatographic- or spectrometric-based methods have already been used to analyze the lipid-soluble components in SMB. However, the methodology of qNMR on the extracts of fresh SMB root has not been verified so far. The purpose of this study was to establish a fast and simple method to quantify the tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh Salvia Miltiorrhiza Bunge root without any pre-purification steps using 1H-NMR spectroscopy. The process is as follows: first, 70% methanol aqueous extracts of fresh Salvia Miltiorrhiza Bunge roots were quantitatively analyzed for tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone using 1H-NMR spectroscopy. Different internal standards were tested and the validated method was compared with HPLC. 3,4,5-trichloropyridine was chosen as the internal standard. Twelve samples of Salvia Miltiorrhiza Bunge were quantitatively analyzed by qNMR and HPLC respectively. Then, the results were analyzed by chemometric approaches. This NMR method offers a fast, stable, and accurate analysis of four ketones: tanshinone I, tanshinone IIA, dihydrotanshinone, and cryptotanshinone in fresh roots of Salvia Miltiorrhiza Bunge.

scholarly journals Metabolism of Tanshinone IIA, Cryptotanshinone and Tanshinone I from Radix Salvia Miltiorrhiza in Zebrafish

Molecules ◽  
2012 ◽  
Vol 17 (7) ◽  
pp. 8617-8632 ◽  
Author(s):  
Yingjie Wei ◽  
Ping Li ◽  
Changmei Wang ◽  
Yunru Peng ◽  
Luan Shu ◽  
...  
Keyword(s):  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Tanshinone I ◽  
Radix Salvia Miltiorrhiza

Simultaneous Determination of Cryptotanshinone, Tanshinone IA and Tanshinone IIA in Three Processing Products of White Flower S. Miltiorrhiza by HPLC

2012 ◽  
Vol 554-556 ◽  
pp. 2037-2040
Author(s):  
Yu Qin Li ◽  
Jing Zhao ◽  
Rui Duan ◽  
Bao Xiu Jia ◽  
Gui Rong You ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Salvia Miltiorrhiza ◽  
Correlation Coefficients ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
White Flower ◽  
Tanshinone Iia ◽  
Regression Equations

A reversed-phase high performance liquid chromatographic method was established for the simultaneous determination of tanshinones in three processing products of white flower Radix salvia miltiorrhiza. Cryptotanshinone, tanshinone IA and tanshinone IIA were successfully separated on a Yilite C18 column (250 mm x 4.6 mm, 5 µm). The mobile phase was a mixture of methanol, water, tetrahydrofuran and glacial acetic acid (70:24:5:1, v/v/v/v), employing isocratic elution at a flow rate of 1.0 mL/min. Detection was 254 nm. Regression equations revealed good linear relationship between the peak areas of the compounds and their concentrations (correlation coefficients: 0.9994 for cryptotanshinone, 0.9996 for tanshinone I A and 0.9996 for tanshinone IIA). The recoveries were between 98.03 % and 103.1 %. The method is simple, accurate and effective and can be used to determine the contents of tanshinones in processing products of Salvia miltiorrhiza bge. f. alba, and the contents of three tanshinones presented the stir-frying processing＜the crude drugs＜the wine prosessing.

Enhancement of solubility and dissolution rate of cryptotanshinone, tanshinone I and tanshinone IIA extracted from Salvia miltiorrhiza

2012 ◽  
Vol 35 (8) ◽  
pp. 1457-1464 ◽  
Author(s):  
Hang Yu ◽  
Robhash Kusam Subedi ◽  
Pushp R. Nepal ◽  
Yoon G. Kim ◽  
Hoo-Kyun Choi
Keyword(s):  
Dissolution Rate ◽  
Salvia Miltiorrhiza ◽  
Tanshinone Iia ◽  
Tanshinone I
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