scholarly journals Inhibition of Osteoarthritis by Adipose-Derived Stromal Cells Overexpressing Fra-1 in Mice

2015 ◽  
Vol 68 (1) ◽  
pp. 138-151 ◽  
Author(s):  
Kay Schwabe ◽  
Mireia Garcia ◽  
Kenia Ubieta ◽  
Nicole Hannemann ◽  
Bettina Herbort ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Adipose Derived Stromal Cells

Fluorescent Immortalized Human Adipose Derived Stromal Cells (hASCs-TS/GFP+) for Studying Cell Drug Delivery Mediated by Microvesicles

2017 ◽  
Vol 17 (11) ◽  
Author(s):  
Valentina Cocce ◽  
Luigi Balducci ◽  
Maria L. Falchetti ◽  
Luisa Pascucci ◽  
Emilio Ciusani ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells

scholarly journals Salidroside promoted osteogenic differentiation of adipose-derived stromal cells through Wnt/β-catenin signaling pathway

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Xiao-hua Li ◽  
Fu-ling Chen ◽  
Hong-lin Shen
Keyword(s):  
Western Blot ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Differentially Expressed Genes ◽  
Stromal Cells ◽  
Differentially Expressed ◽  
Calcium Deposition ◽  
Western Blot Assay ◽  
Adipose Derived Stromal Cells ◽  
Interfering Rna

Abstract Background Bone disease causes short-term or long-term physical pain and disability. It is necessary to explore new drug for bone-related disease. This study aimed to explore the role and mechanism of Salidroside in promoting osteogenic differentiation of adipose-derived stromal cells (ADSCs). Methods ADSCs were isolated and treated with different dose of Salidroside. Cell count kit-8 (CCK-8) assay was performed to assess the cell viability of ADSCs. Then, ALP and ARS staining were conducted to assess the early and late osteogenic capacity of ADSCs, respectively. Then, differentially expressed genes were obtained by R software. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the differentially expressed genes were further analyzed. The expression of OCN, COL1A1, RUNX2, WNT3A, and β-catenin were measured by real-time PCR and Western blot analysis. Last, β-catenin was silenced by small interfering RNA. Results Salidroside significantly increased the ADSCs viability at a dose-response manner. Moreover, Salidroside enhanced osteogenic capacity of ADSCs, which are identified by enhanced ALP activity and calcium deposition. A total of 543 differentially expressed genes were identified between normal and Salidroside-treated ADSCs. Among these differentially expressed genes, 345 genes were upregulated and 198 genes were downregulated. Differentially expressed genes enriched in the Wnt/β-catenin signaling pathway. Western blot assay indicated that Salidroside enhanced the WNT3A and β-catenin expression. Silencing β-catenin partially reversed the promotion effects of Salidroside. PCR and Western blot results further confirmed these results. Conclusion Salidroside promoted osteogenic differentiation of ADSCs through Wnt/β-catenin signaling pathway.

scholarly journals Adipose‐derived stromal cells for nonhealing wounds: Emerging opportunities and challenges

2021 ◽  
Author(s):  
Milena Deptuła ◽  
Agnieszka Brzezicka ◽  
Aneta Skoniecka ◽  
Jacek Zieliński ◽  
Michał Pikuła
Keyword(s):  
Stromal Cells ◽  
Adipose Derived Stromal Cells

In Vivo Bone Formation Following Transplantation of Human Adipose–Derived Stromal Cells That Are Not Differentiated Osteogenically

2008 ◽  
Vol 14 (8) ◽  
pp. 1285-1294 ◽  
Author(s):  
Oju Jeon ◽  
Jong Won Rhie ◽  
Il-Kuen Kwon ◽  
Jae-Hwan Kim ◽  
Byung-Soo Kim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells ◽  
In Vivo Bone Formation
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