Evaluation of bone formation capacities of human adipose-derived stromal cells cultured in platelet growth factor-enriched plasma medium.

2013 ◽  
Author(s):  
Fabien Guilloton ◽  
Vahideh Rabani ◽  
Meadhbh Brennan ◽  
Giulio Bassi ◽  
Mauro Krampera ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Formation ◽  
Stromal Cells ◽  
Plasma Medium ◽  
Adipose Derived Stromal Cells ◽  
Platelet Growth Factor ◽  
Cells Cultured

In Vivo Bone Formation Following Transplantation of Human Adipose–Derived Stromal Cells That Are Not Differentiated Osteogenically

2008 ◽  
Vol 14 (8) ◽  
pp. 1285-1294 ◽  
Author(s):  
Oju Jeon ◽  
Jong Won Rhie ◽  
Il-Kuen Kwon ◽  
Jae-Hwan Kim ◽  
Byung-Soo Kim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells ◽  
In Vivo Bone Formation

scholarly journals Comparative Analysis of Adipose-Derived Stromal Cells and Their Secretome for Auricular Cartilage Regeneration

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Se-Joon Oh ◽  
Kyung-Un Choi ◽  
Sung-Won Choi ◽  
Sung-Dong Kim ◽  
Soo-Keun Kong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Stromal Cells ◽  
Collagen Type ◽  
Cartilage Regeneration ◽  
Cartilage Defects ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Auricular Cartilage ◽  
Adipose Derived Stromal Cells

Adipose-derived stromal cells (ADSCs) can repair auricular cartilage defects. Furthermore, stem cell secretome may also be a promising biological therapeutic option, which is equal to or even superior to the stem cell. We explored the therapeutic efficacies of ADSCs and their secretome in terms of rabbit auricular cartilage regeneration. ADSCs and their secretome were placed into surgically created auricular cartilage defects. After 4 and 8 weeks, the resected auricles were histopathologically and immunohistochemically examined. We used real-time PCR to determine the levels of genes expressing collagen type II, transforming growth factor-β1 (TGF-β1), and insulin-like growth factor-1 (IGF-1). ADSCs significantly improved auricular cartilage regeneration at 4 and 8 weeks, compared to the secretome and PBS groups, as revealed by gross examination, histopathologically and immunohistochemically. ADSCs upregulated the expression of collagen type II, TGF-β1, and IGF-1 more so than did the secretome or PBS. The expression levels of collagen type II and IGF-1 were significantly higher at 8 weeks than at 4 weeks after ADSC injection. Although ADSCs thus significantly enhanced new cartilage formation, their secretome did not. Therefore, ADSCs may be more effective than their secretome in the repair of auricular cartilage defect.

107 PRODUCTION OF EPIDERMAL GROWTH FACTOR BY BOVINE ENDOMETRIAL CELLS AND ITS EFFECTS ON EMBRYONIC INTERFERONT AND PROSTAGLANDIN

2015 ◽  
Vol 27 (1) ◽  
pp. 146 ◽  
Author(s):  
T. J. Acosta ◽  
K. Takatsu
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Stromal Cells ◽  
Pge2 Production ◽  
Bioactive Molecules ◽  
Significant Difference ◽  
Epidermal Growth ◽  
Maternal Recognition Of Pregnancy ◽  
Cells Cultured

A dynamic interaction between bioactive products of the embryo (blastocyst) and the endometrium is crucial for the successful establishment of pregnancy. In ruminants, the principal signal for maternal recognition of pregnancy is interferon-τ (IFNT) secreted by the trophoectoderm between Days 8 and 20 post-fertilization. Epidermal growth factor (EGF) produced by the endometrium acting through EGF receptors (EGFR) present in the blastocyst seems to regulate embryonic production of IFNT. Epidermal growth factor and IFNT have been shown to play crucial roles in controlling luteolytic prostaglandin (PG) F2α (PGF) and luteotropic PGE2 production by bovine endometrium. However, it is unknown how these bioactive molecules regulate uterine function during maternal recognition of pregnancy. To clarify the main source of EGF in bovine endometrium and the mechanisms regulating the interaction between the hatched blastocyst and maternal uterine environment, the production of EGF by cultured endometrial epithelial and stromal cells and the effects of EGF on embryonic IFNT and PG were investigated. In addition, the effects of EGF on PGE2 and PGF production by cultured epithelial or stromal cells were examined. Endometrial epithelial and stromal cells were enzymatically isolated on the day of ovulation, seeded at a density of 100 000 viable cells mL–1, and cultured at 38°C in a humidified atmosphere of 5% CO2 in air. After the cells reached 90% confluence, they were cultured in the presence or absence of EGF (0.1, 1.0, 10, and 100 ng mL–1) for 24 h. Cells cultured in the absence of EGF and their cultured media were collected separately for protein analysis. Hatched bovine blastocysts (Days 8–10) were also cultured and exposed to EGF (1, 10, and 100 ng mL–1) for 24 h. Protein concentrations of EGF and IFNT in the cultured media were determined by commercial enzyme immunoassay kit. Hormonal concentrations were analysed by ANOVA followed by Fisher's protected least-significant difference procedure (PLSD) as a multiple comparison test by StatView (Abacus Concepts Inc., Berkeley, CA, USA). The concentration of EGF in the culture media of epithelial cells cultured in the absence of EGF was significantly (P < 0.05) higher than in the cultured media of endometrial stromal cells. Epidermal growth factor (10 and 100 ng mL–1) increased embryonic production of IFNT and luteotropic PGE2 production but not luteolytic PGF by hatched blastocyst. EGF (100 ng mL–1) increased both PGE2 and PGF production (P < 0.05) by cultured endometrial epithelial and stromal cells. The overall results suggest that endometrial epithelial cells rather than stromal cells are the main source of EGF. Epidermal growth factor produced by epithelial cells stimulates the production of IFNT by bovine trophoblasts. The capacity of conceptus to increase IFNT and luteotropic PGE2 production rather than luteolytic PGF in response to EGF stimulation may be essential for the establishment of pregnancy in cattle.

scholarly journals Dura Mater Stimulates Human Adipose-Derived Stromal Cells to Undergo Bone Formation in Mouse Calvarial Defects

Stem Cells ◽  
2011 ◽  
Vol 29 (8) ◽  
pp. 1241-1255 ◽  
Author(s):  
Benjamin Levi ◽  
Emily R. Nelson ◽  
Shuli Li ◽  
Aaron W. James ◽  
Jeong S. Hyun ◽  
...  
Keyword(s):  
Bone Formation ◽  
Dura Mater ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells ◽  
Calvarial Defects

Adipose-Derived Stromal Cells (ADSCs) Promote Bone Formation In Vivo Not by Transforming to Osteoblasts

2018 ◽  
Vol 8 (5) ◽  
pp. 624-631
Author(s):  
Xiaoyu Li ◽  
Jinfeng Yao ◽  
Xinya Du ◽  
Wei Jing ◽  
Weidong Tian
Keyword(s):  
Bone Formation ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells
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