Preconditioning Human Adipose-Derived Stromal Cells on Decellularized Adipose Tissue Scaffolds within a Perfusion Bioreactor Modulates Cell Phenotype and Promotes a Pro-Regenerative Host Response

2020 ◽  
Author(s):  
Tim T.Y. Han ◽  
John T. Walker ◽  
Aaron Grant ◽  
Gregory A. Dekaban ◽  
Lauren Elizabeth Flynn
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Host Response ◽  
Tissue Scaffolds ◽  
Cell Phenotype ◽  
Perfusion Bioreactor ◽  
Adipose Derived Stromal Cells

scholarly journals Preconditioning Human Adipose-Derived Stromal Cells on Decellularized Adipose Tissue Scaffolds Within a Perfusion Bioreactor Modulates Cell Phenotype and Promotes a Pro-regenerative Host Response

2021 ◽  
Vol 9 ◽  
Author(s):  
Tim Tian Y. Han ◽  
John T. Walker ◽  
Aaron Grant ◽  
Gregory A. Dekaban ◽  
Lauren E. Flynn
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Soft Tissue Augmentation ◽  
Cell Phenotype ◽  
Perfusion Bioreactor ◽  
Bioreactor Culture ◽  
Arginase 1 ◽  
Adipose Derived Stromal Cells ◽  
Human Ascs

Cell-based therapies involving the delivery of adipose-derived stromal cells (ASCs) on decellularized adipose tissue (DAT) scaffolds are a promising approach for soft tissue augmentation and reconstruction. Our lab has recently shown that culturing human ASCs on DAT scaffolds within a perfusion bioreactor prior to implantation can enhance their capacity to stimulate in vivo adipose tissue regeneration. Building from this previous work, the current study investigated the effects of bioreactor preconditioning on the ASC phenotype and secretory profile in vitro, as well as host cell recruitment following implantation in an athymic nude mouse model. Immunohistochemical analyses indicated that culturing within the bioreactor increased the percentage of ASCs co-expressing inducible nitric oxide synthase (iNOS) and arginase-1 (Arg-1), as well as tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10), within the peripheral regions of the DAT relative to statically cultured controls. In addition, bioreactor culture altered the expression levels of a range of immunomodulatory factors in the ASC-seeded DAT. In vivo testing revealed that culturing the ASCs on the DAT within the perfusion bioreactor prior to implantation enhanced the infiltration of host CD31+ endothelial cells and CD26+ cells into the DAT implants, but did not alter CD45+F4/80+CD68+ macrophage recruitment. However, a higher fraction of the CD45+ cell population expressed the pro-regenerative macrophage marker CD163 in the bioreactor group, which may have contributed to enhanced remodeling of the scaffolds into host-derived adipose tissue. Overall, the findings support that bioreactor preconditioning can augment the capacity of human ASCs to stimulate regeneration through paracrine-mediated mechanisms.

Perfusion bioreactor culture of adipose-derived stromal cells on decellularized adipose tissue scaffolds enhances in vivo regeneration

2020 ◽  
Author(s):  
Tim Tian Y. Han ◽  
Lauren E. Flynn
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Unmet Need ◽  
Perfusion Bioreactor ◽  
Bioreactor Culture ◽  
Adipose Tissue Engineering ◽  
Adipose Derived Stromal Cells ◽  
The Impact

AbstractAdipose tissue engineering holds promise to address the unmet need in plastic and reconstructive surgery for strategies that promote the stable and predictable regeneration of adipose tissue for volume augmentation applications. Previous studies have demonstrated that decellularized adipose tissue (DAT) scaffolds can provide a pro-adipogenic microenvironment, and that seeding with adipose-derived stromal cells (ASCs) can enhance in vivo angiogenesis and adipogenesis within DAT implants. Recognizing that bioreactor systems can promote cell expansion and infiltration on tissue-engineered scaffolds, this study evaluated the effects of culturing human ASCs on DAT scaffolds within a perfusion bioreactor. Using this system, the impact of both shear stress stimulation and hypoxic preconditioning were explored in vitro and in vivo. Initial studies compared the effects of 14 days of culture within the perfusion bioreactor under 2% O2 or ~20% O2 on human ASC expansion and hypoxia inducible factor 1 alpha (HIF-1α) expression in vitro relative to static cultured controls. The findings indicated that culturing within the bioreactor under 2% O2 significantly increased ASC proliferation on the DAT, with a higher cell density observed in the scaffold periphery. HIF-1α expression was significantly higher when the scaffolds were cultured under 2% O2. Subsequent characterization in a subcutaneous implant model in athymic nude mice revealed that in vivo angiogenesis and adipogenesis were markedly enhanced when the ASCs were cultured on the DAT within the perfusion bioreactor under 2% O2 for 14 days prior to implantation relative to the other culture conditions, as well as additional freshly-seeded and unseeded DAT control groups. Overall, dynamic culture within the perfusion bioreactor system under hypoxia represents a promising approach for preconditioning ASCs on DAT scaffolds to enhance their capacity to stimulate blood vessel formation and infiltration, as well as host-derived adipose tissue regeneration.

The Development of Facial Lipofilling from a Historical Point of View

2019 ◽  
Vol 35 (04) ◽  
pp. 358-367 ◽  
Author(s):  
Joris A. van Dongen ◽  
Joeri van Boxtel ◽  
Martin C. Harmsen ◽  
Hieronymus P. Stevens
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Stromal Vascular Fraction ◽  
Point Of View ◽  
Congenital Defects ◽  
Skin Rejuvenation ◽  
Adipose Derived Stromal Cells ◽  
Enzymatic Isolation ◽  
The Face ◽  
Mechanical Isolation

AbstractLipofilling, the transplantation of adipose tissue, has already been used since the end of the 19th century. For decades, lipofilling was used to restore loss of volume due to aging, trauma, or congenital defects. Later on, the indications for the use of lipofilling expanded by treating aged skin, scars, and improving wound healing. The expansion was caused by the discovery of adipose derived stromal cells (ASCs) in adipose tissue and the development of very fine harvesting and injection cannulas which made it possible to inject small adipose tissue particles in small volume areas, such as the face. ASCs are multipotent stromal cells which reside in the stromal vascular fraction (SVF) of adipose tissue and are able to differentiate in multiple cell lineages and secrete a plurality of growth factors with regenerative potentials. The discovery of ASCs led toward more experimental cell-based therapies, that is, ASCs or SVF isolated by means of enzymatic isolation procedures. Later on, enzymatic isolation procedures were forbidden in many countries by legislation and were replaced by mechanical isolation procedures, such as the Nanofat and Fractionation of Adipose Tissue (FAT) procedures. The Nanofat procedure has been extensively investigated, especially as treatment for skin rejuvenation in the face. Though, substantial evidence is lacking for using facial lipofilling or any therapeutic component, that is, ASCs or SVF for skin rejuvenation to date. In contrast, facial lipofilling to restore loss of volume seems to be promising.

Adipose Tissue Engineering by Human Adipose-Derived Stromal Cells

2006 ◽  
Vol 183 (3) ◽  
pp. 133-140 ◽  
Author(s):  
Liu Hong ◽  
Ioana A. Peptan ◽  
Aylin Colpan ◽  
Joseph L. Daw
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Adipose Tissue Engineering ◽  
Adipose Derived Stromal Cells
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