scholarly journals Specific autoantibodies precede the symptoms of rheumatoid arthritis: A study of serial measurements in blood donors

2004 ◽  
Vol 50 (2) ◽  
pp. 380-386 ◽  
Author(s):  
Markus M. J. Nielen ◽  
Dirkjan van Schaardenburg ◽  
Henk W. Reesink ◽  
Rob J. van de Stadt ◽  
Irene E. van der Horst-Bruinsma ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Blood Donors ◽  
Serial Measurements

scholarly journals Lipids and inflammation: serial measurements of the lipid profile of blood donors who later developed rheumatoid arthritis

2006 ◽  
Vol 66 (2) ◽  
pp. 184-188 ◽  
Author(s):  
V P van Halm ◽  
M M J Nielen ◽  
M T Nurmohamed ◽  
D van Schaardenburg ◽  
H W Reesink ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Lipid Profile ◽  
Blood Donors ◽  
Serial Measurements

scholarly journals AB0008 THE IMPACT OF STAT4 RS7574865, IL6 RS1800795, IL6R RS2228145 AND RS4845618 ON RHEUMATOID ARTHRITIS SUSCEPTIBILITY IN BELARUSIAN POPULATION

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1307.1-1308
Author(s):  
E. Siniauskaya ◽  
T. Kuzhir ◽  
V. Yagur ◽  
R. Goncharova
Keyword(s):  
Rheumatoid Arthritis ◽  
Blood Donors ◽  
Genetic Model ◽  
Online Tool ◽  
Genotype Frequencies ◽  
Systemic Disorder ◽  
Autoimmune Pathogenesis ◽  
The Impact ◽  
Arthritis Susceptibility

Background:Rheumatoid arthritis (RA) is a chronic systemic disorder of the connective tissue of still unknown aetiology and complex autoimmune pathogenesis that primarily affects small joints. HLA alleles provide for 11-37% of the RA heritability, suggesting the substantial role of the non-HLA loci in genetic predisposition to RA. Among non-HLA loci,IL6, IL6RandSTAT4genes attract attention, however, the data concerning their influence on RA risk are somewhat contradictory.Objectives:The aim of the study was to analyze the involvement of four SNPs (STAT4rs7574865,IL6rs1800795,IL6Rrs2228145 and rs4845618) in RA susceptibility.Methods:187 patients diagnosed with RA (mean age 58.2 ± 11.9), and 380 healthy blood donors (mean age 37.18 ± 10.69 years) were included into the study. DNA extraction from peripheral blood samples was performed using the phenol-chloroform method. SNPs were genotyped using the real-time PCR with fluorescent probes. The allele and genotype frequencies were compared using the χ2 test. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated using the VassarStats online tool.Results:Utilizing recessive genetic model we found an association between TT genotype ofSTAT4rs7574865 (OR = 2.362; 95%CI [1.0378 – 5.376], p = 0.038) and RA. ForIL6rs1800795, it was found that CC genotype had significantly higher frequency among patients with rheumatoid arthritis as compared to that in controls (OR = 1.52; 95%CI [1.02 – 2.27], p = 0.0456). No associations ofIL6Rrs2228145 and rs4845618 SNPs with risk of RA were found in the total group of patients vs. controls. It was also shown thatIL6rs1800795 CC genotype frequency was significantly higher among the patients with RF-negative status (p = 0.0019).Conclusion:Thus, we provide evidence for association of theSTAT4rs7574865 andIL6rs1800795 variants with risk of RA in the Belarusian population, some features of interplay being revealed between gene polymorphisms analyzed and RA antibody status. Abovementioned SNPs may contribute to RA genetic susceptibility in the Belarusian population.Disclosure of Interests:None declared

A2.18 Induction and characterisation of the dominant IL-10 producing B cell subset in healthy blood donors and rheumatoid arthritis patients

2016 ◽  
Vol 75 (Suppl 1) ◽  
pp. A22.2-A22
Author(s):  
ZS Bankó ◽  
J Pozsgay ◽  
M Tóth ◽  
T Gáti ◽  
G Nagy ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
B Cell ◽  
Blood Donors ◽  
Cell Subset

scholarly journals Induction and Differentiation of IL-10–Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients

2017 ◽  
Vol 198 (4) ◽  
pp. 1512-1520 ◽  
Author(s):  
Zsuzsanna Bankó ◽  
Judit Pozsgay ◽  
Dániel Szili ◽  
Mária Tóth ◽  
Tamás Gáti ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
B Cells ◽  
Blood Donors ◽  
Regulatory B Cells

scholarly journals NETs analysed by novel calprotectin‐based assays in blood donors and patients with multiple myeloma or rheumatoid arthritis: A pilot study

2020 ◽  
Vol 91 (5) ◽  
Author(s):  
Magne Kristoffer Fagerhol ◽  
Egil Johnson ◽  
Jon‐Magnus Tangen ◽  
Ivana Hollan ◽  
Mohammad Reza Mirlashari ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Multiple Myeloma ◽  
Pilot Study ◽  
Blood Donors

Noninvasive Diagnosis of Pulmonary Hemorrhage in Rheumatoid Arthritis

PEDIATRICS ◽  
1983 ◽  
Vol 72 (5) ◽  
pp. 720-723
Author(s):  
HUGH M. O'BRODOVICH ◽  
R. CLIFTON WAY ◽  
MAUREEN ANDREW ◽  
PETER B. DENT
Keyword(s):  
Rheumatoid Arthritis ◽  
Respiratory Tract ◽  
Pulmonary Hemorrhage ◽  
Chest Roentgenogram ◽  
Noninvasive Diagnosis ◽  
Upper Respiratory Tract ◽  
Pulmonary Uptake ◽  
Atrial Size ◽  
Large Airways ◽  
Serial Measurements

The noninvasive diagnosis of pulmonary hemorrhage usually relies upon the presence of hemoptysis or hemosiderin-laden macrophages in the sputum. Respiratory tract secretions, however, may be contaminated with blood during passage through the large airways or upper respiratory tract, and hemosiderin-laden macrophages may be found in the sputum of patients with other diseases such as pulmonary edema. In addition, pulmonary hemorrhage may occur without associated hemoptysis or significant changes in the chest roentgenogram.1 We have used serial measurements of the pulmonary uptake of carbon monoxide combined with M-mode echocardiography to monitor left atrial size in order to document pulmonary hemorrhage in an 11-year-old girl with rheumatoid arthritis.

Association Between the Aggrecan Gene and Rheumatoid Arthritis

2008 ◽  
Vol 35 (12) ◽  
pp. 2325-2328 ◽  
Author(s):  
THAIS B. de SOUZA ◽  
ELISA F. MENTZ ◽  
CLAITON V. BRENOL ◽  
RICARDO M. XAVIER ◽  
JOÃO C.T. BRENOL ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Blood Donors ◽  
Tandem Repeats ◽  
Dna Amplification ◽  
Variable Number ◽  
Control Group ◽  
Vntr Polymorphism ◽  
Study Population ◽  
Polymerase Chain ◽  
Genetic And Environmental Factors

ObjectiveGenetic and environmental factors seem to be involved in the onset of rheumatoid arthritis (RA). We analyzed whether a variable number of tandem repeats (VNTR) polymorphism in the aggrecan gene was associated to RA.MethodsThe study population comprised 170 European-derived Brazilian patients with diagnosis of RA. The control group comprised 148 European-derived Brazilian healthy blood donors. The aggrecan VNTR polymorphism was genotyped by DNA amplification by polymerase chain reaction, followed by electrophoresis in polyacrylamide gel.ResultsThere was a statistically significant higher frequency of alleles of shorter length in the patient group compared to controls (p = 0.001), suggesting that individuals carrying short alleles are more likely to develop RA. There was no association between short alleles and clinical characteristics of RA.ConclusionOur results provide evidence of an association between the aggrecan gene VNTR polymorphism and RA.

scholarly journals Increased levels of C-reactive protein in serum from blood donors before the onset of rheumatoid arthritis

2004 ◽  
Vol 50 (8) ◽  
pp. 2423-2427 ◽  
Author(s):  
Markus M. J. Nielen ◽  
Dirkjan Van Schaardenburg ◽  
Henk W. Reesink ◽  
Jos W. R. Twisk ◽  
Rob J. Van De Stadt ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Blood Donors ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals AB0024 ASSOCIATION OF IL6 RS1800795 BUT NOT IL6R RS2228145, RS4845618 AND STAT4 RS7574865 POLYMORPHISMS WITH CHLAMYDIA-ASSOCIATED RHEUMATOID ARTHRITIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1315.2-1315
Author(s):  
T. Zybalova ◽  
V. Yagur ◽  
R. Goncharova ◽  
N. Soroka ◽  
N. Dostanko ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Chlamydial Infection ◽  
Blood Donors ◽  
Statistical Significance ◽  
Diagnostic Odds Ratio ◽  
Fisher Exact Test ◽  
Bonferroni Correction ◽  
Research Support ◽  
Exact Test ◽  
Healthy Donors

Background:Rheumatoid arthritis (RA), associated with Chlamydial Infection, has some clinical and immunological particulars that interfere with the early diagnosis and require significant changes in treatment strategy [1].Objectives:To estimate the distribution of some non-HLA genetic markers such as STAT4 rs7574865, IL6 rs1800795, IL6R rs2228145 and rs4845618 in Chlamydia positive and negative RA patients and healthy controls.Methods:We examined 380 healthy blood donors and 187 RA patients classified according to the ACR/EULAR 2010 criteria for RA [2]. Twenty-three of the RA patients were positive for Chlamidia trachomatis (n=17) or Chlamidia pneumonia (n=6) persistence. DNA from peripheral blood samples was extracted by phenol-chloroform method. SNPs were genotyped by the real-time PCR with fluorescent probes. Statistical significance of SNPs’ frequency was estimated by two-way Fisher exact test (F, p2-t) with Bonferroni correction for multiple comparisons (pcor). Moreover, diagnostic odds ratio (dOR), the likelihood ratio of positive (LR+) and negative (LR–) tests and corresponding confidence intervals (CI) were calculated.Results:We revealed statistically significant increase of genotype СС frequency (IL6 rs1800795) in Chlamydia-associated RA (60.9%) vs healthy donors (20.7%): p2-t=0.000065; pcor=0.00026; dOR=5.95 (CI95%2.53-13.94); LR+=2.94 (CI95%1.90-3.29); LR–=0.49 (CI95%0.28-0.75) as well as in Chlamydia-associated RA (60.9%) vs Chlamydia-negative RA (23.9%): p2-t=0.00051; pcor=0.002; dOR=4.99 (CI95%2.04-12.16); LR+=2.56 (CI95%1.60-3.57); LR–=0.51 (CI95%0.29-0.78). Significant differences in STAT4 rs7574865, IL6R rs2228145 and IL6R rs4845618 distribution between studied groups were not found.Conclusion:Our data suggest the association between СС genotype of IL6 rs1800795 and Chlamydia-associated RA.References:[1]Soroka N.F. Rheumatoid Arthritis, associated with Chlamydial infection // Healthcare 2009; 1: 5-9.[2]Aletaha D. et al. 2010 Rheumatoid arthritis classification criteria// Arthritis Rheum 2010; 62 (9): 2569-81.Disclosure of Interests:Tatiana Zybalova: None declared, Viktor Yagur: None declared, Roza Goncharova: None declared, Nikolaj Soroka Grant/research support from: JSC BIOCAD, Natalia Dostanko: None declared, Valery Apanasovich: None declared, Anastasiya Tushina: None declared

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